Cytokine Adsorber Use during DCD Heart Perfusion Counteracts Coronary Microvascular Dysfunction
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Anesthesia
2.2. Donation after Circulatory Death Model and Harvesting of Hearts
2.3. Blood Perfusion and Study Groups
2.4. Microvascular Functional Assessment
2.5. Cytokine Profiling
2.6. Immunohistochemical Staining
2.7. Gene Expression Analysis
2.8. Statistical Analysis and Machine Learning Algorithm
3. Results
3.1. Microvascular Function
3.2. Perfusion Pressure
3.3. Cytokine Profile
3.4. Oxidative Stress and Endothelial Injury
3.5. Gene Expression
4. Discussion
4.1. Microvascular Functional Effects
4.2. Cytokine Profile
4.3. Oxidative Stress and Endothelial Injury
4.4. Gene Expression
4.5. Limitations
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
BP | blood perfusion |
CAV1 | caveolin 1 |
CD-31 | cluster of differentiation-31 |
CPP | coronary perfusion pressure |
DCD | donation after circulatory death |
eNOS | endothelial nitric oxide synthase |
FGF2 | fibroblast growth factor 2 |
HIF1A | hypoxia inducible factor 1a |
ICAM | intracellular cell adhesion molecule-1 |
IL | interleukin, interleukine |
IRI | ischemia/reperfusion injury |
ITGB1 | integrin beta 1 |
LDP | laser-doppler-perfusion |
CMVD | coronary microvascular dysfunction |
NOS3 | nitric oxide synthase 3, eNOS |
PECAM-1 | platelet endothelial cell adhesion molecule-1 |
PLAT | plasminogen activator |
PLAU | plasminogen activator urokinase |
RLDP | relative LDP |
ROS | reactive oxygen species |
SERPRINE1 | plasminogen activator inhibitor 1 |
VCAM | vascular cell adhesion molecule-1 |
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Saemann, L.; Hoorn, F.; Georgevici, A.-I.; Pohl, S.; Korkmaz-Icöz, S.; Veres, G.; Guo, Y.; Karck, M.; Simm, A.; Wenzel, F.; et al. Cytokine Adsorber Use during DCD Heart Perfusion Counteracts Coronary Microvascular Dysfunction. Antioxidants 2022, 11, 2280. https://doi.org/10.3390/antiox11112280
Saemann L, Hoorn F, Georgevici A-I, Pohl S, Korkmaz-Icöz S, Veres G, Guo Y, Karck M, Simm A, Wenzel F, et al. Cytokine Adsorber Use during DCD Heart Perfusion Counteracts Coronary Microvascular Dysfunction. Antioxidants. 2022; 11(11):2280. https://doi.org/10.3390/antiox11112280
Chicago/Turabian StyleSaemann, Lars, Fabio Hoorn, Adrian-Iustin Georgevici, Sabine Pohl, Sevil Korkmaz-Icöz, Gábor Veres, Yuxing Guo, Matthias Karck, Andreas Simm, Folker Wenzel, and et al. 2022. "Cytokine Adsorber Use during DCD Heart Perfusion Counteracts Coronary Microvascular Dysfunction" Antioxidants 11, no. 11: 2280. https://doi.org/10.3390/antiox11112280