Selenium Nanoparticles (SeNPs) Immunomodulation Is More Than Redox Improvement: Serum Proteomics and Transcriptomic Analyses
Abstract
:1. Introduction
2. Materials and Methods
2.1. Selenium Nanoparticles (SeNPs) Preparation and Characterization
2.2. SeNPs Diet and Trolox Diet Preparation
2.3. Fish Maintenance
2.4. Comparison of Immunomodulation Effects with SeNP and Trolox Diet
2.5. Comparison of Antioxidation Effects with SeNP and Trolox Diet
2.6. Serum Collection and Proteomic Sample Preparation
2.7. Serum Proteomics Analysis
2.8. Transcriptome Analysis
2.9. ICP-MS Analysis
2.10. Statistical Analyses
3. Results
3.1. SeNPs and SeNP Diet Characterization
3.2. Comparison of Immunomodulation Effects with SeNP and Trolox Diet
3.3. Comparison of Antioxidation Effects with SeNP and Trolox Diet
3.4. Serum Proteomic Analysis
3.5. Canonical Pathway and Molecular Network Analyses from Serum Proteomics
3.6. Uptake of Se and Transcriptomic Response of Kidney and Liver
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Analysis | ID | Molecules in Network | Score | Focus Molecules | Top Diseases and Functions |
---|---|---|---|---|---|
Healthy (SeNP diet vs. Control diet) | 1 | A2M, adhesion molecule, AKR1B1, APOA1, Cg, Cpla2, F10, GGCT, GLRX, glutathione peroxidase, GPX1, GPX3, GPX4, Growth hormone, GST, GSTM3, GSTP1, Hif, Ldh (complex), LDHA, LDHB, LDL, MTPN, NFkβ (complex), peroxidase (miscellaneous), PGAM2, PRDX1, PRDX2, PRDX5, PRDX6, PROC, SHBG, Sod, SOD1, SYK/ZAP | 45 | 22 | Free Radical Scavenging, Small Molecule Biochemistry, Drug Metabolism |
2 | AHSG, Alpha 1 antitrypsin, APOA4, APOB, BHMT, Ces, CES1, CETP, CFH, CP, creatine kinase, ERK1/2, ESD, Ferritin, FGA, GC, HDL, HDL-cholesterol, hemoglobin, HPX, ITIH4, ITLN1, MHC Class II (complex), Nos, Nr1h, PRKAA, RBP4, SERPINA1, SERPINA9, SERPINC1, SERPINF2, SH3BGRL, TF, VLDL, VLDL-cholesterol | 43 | 21 | Lipid Metabolism, Small Molecule Biochemistry, Vitamin and Mineral Metabolism | |
3 | Akt, C3, C5, C6, C7, C8, C1q, C3-Cfb, C5-C6-C7, C5-C6-C7-C8, C5-C6-C7-C8-C9, C8A, C8B, C8G, CFI, Collagen Alpha1, Collagen type IV, Collagen(s), Complement, Complement component 1, CSTB, elastase, Fibrin, Fibrinogen, HABP2, HBE1, HBZ, Kallikrein, Laminin (complex), LRG1, MAC, NME3, Pdi, PLG, SERPING1 | 30 | 16 | Immunological Disease, Developmental Disorder, Hereditary Disorder | |
Disease (SeNP diet vs. Control diet) | 1 | 20s proteasome, ACLY, AK1, ALDOA, AMPK, Dynamin, EGLN, ENO3, ERK, GAPDH, GAPDHS, GOT, GOT1, Hif1, Ikb, Immunoproteasome Pa28/20s, LDHA, LDHB, LRG1, NDPK, NME1, NME2, NME3, PGK1, PGM1, PRDX2, PRDX5, Proteasome PA700/20s, PSMA, PSMA4, PSMA5, PSMA8, PSMB3, Rsk, SOD1 | 44 | 22 | Carbohydrate Metabolism, Nucleic Acid Metabolism, Small Molecule Biochemistry |
2 | AHSG, Alpha 1 antitrypsin, AMBP, APOA4, APOB, CES1, CETP, CFH, CP, creatine kinase, ERK1/2, Ferritin, FGA, GC, HBZ, HDL, HDL-cholesterol, hemoglobin, HPX, Iti, ITIH3, ITIH4, ITLN1, MHC Class II (complex), Nos, Nr1h, PRKAA, RBP4, SERPINA1, SERPINA9, SERPINC1, SERPINF2, TF, VLDL, VLDL-cholesterol | 41 | 21 | Lipid Metabolism, Small Molecule Biochemistry, Vitamin and Mineral Metabolism | |
3 | A2M, C6, C7, C3-Cfb, C5-C6-C7, C5-C6-C7-C8, C5-C6-C7-C8-C9, C8A, C8B, CAPNS1, CFB, CFD, CFI, CFP, chymotrypsin, coagulation factor, CORO1A, Ecm, elastase, F9, F10, glutathione peroxidase, Hif, Kallikrein, Ldh (complex), MASP2, NFkβ (complex), PAPSS2, PLG, PROC, PRSS2, Serine Protease, SERPING1, SOD3, trypsin | 38 | 20 | Immunological Disease, Developmental Disorder, Hereditary Disorder |
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Xia, I.F.; Kong, H.-K.; Wu, M.M.H.; Lu, Y.; Wong, K.-H.; Kwok, K.W.H. Selenium Nanoparticles (SeNPs) Immunomodulation Is More Than Redox Improvement: Serum Proteomics and Transcriptomic Analyses. Antioxidants 2022, 11, 964. https://doi.org/10.3390/antiox11050964
Xia IF, Kong H-K, Wu MMH, Lu Y, Wong K-H, Kwok KWH. Selenium Nanoparticles (SeNPs) Immunomodulation Is More Than Redox Improvement: Serum Proteomics and Transcriptomic Analyses. Antioxidants. 2022; 11(5):964. https://doi.org/10.3390/antiox11050964
Chicago/Turabian StyleXia, Ivan Fan, Hang-Kin Kong, Margaret M. H. Wu, Yishan Lu, Ka-Hing Wong, and Kevin W. H. Kwok. 2022. "Selenium Nanoparticles (SeNPs) Immunomodulation Is More Than Redox Improvement: Serum Proteomics and Transcriptomic Analyses" Antioxidants 11, no. 5: 964. https://doi.org/10.3390/antiox11050964