Understanding the Mechanism of Action of Melatonin, Which Induces ROS Production in Cancer Cells
, Laura Martinez-Ruiz 1,2, Alba López-RodrÃguez 1,2, DarÃo Acuña-Castroviejo 1,2,3, Iryna Rusanova 1,3,4 and Germaine Escames 1,2,3,*
Round 1
Reviewer 1 Report
Understanding the mechanism of action of melatonin that in-2 duces ROS production in cancer cells.
There are many recent reviews concerning the use of melatonin in the treatment of cancer and its dual activity as antioxidant and pro-oxidant in cancer cells. However, this review is concerned about the biochemical mechanisms involved. This s submission by Escames et al, aims to describe and clarify the proposed mechanism of action of melatonin inducing ROS production in cancer cells in order to propose future anti neoplastic clinical applications.
The manuscript is well written and organized, recent references are also included. I suggest considering the following reference: https://doi.org/10.1016/j.biochi.2022.05.008.
Therefore I suggest to publish in the present form after minor mispelling corrections.
Author Response
First, we would like to return the revised manuscript entitled” “Understanding the mechanism of action of melatonin that induces ROS production in cancer cells” (Manuscript ID antioxidants-1864450) to the Journal of Antioxidants for the Special Issue*: Free-Radical Scavenging and Antioxidant Properties of Melatonin, for its consideration of publication. We would like to thank reviewers for critically examining this manuscript and providing us invaluable comments and suggestions. We respond to each of the reviewer’s comments and clarified/amended every necessary part. The statements addressing the referee's comments point by point are listed below and the revised text with alterations underlined and red font was uploaded. We highly appreciate your consideration and further process
Amendments to the questions raised by the reviewer #1 to the manuscript ID antioxidants-1864450
There are many recent reviews concerning the use of melatonin in the treatment of cancer and its dual activity as antioxidant and pro-oxidant in cancer cells. However, this review is concerned about the biochemical mechanisms involved. This submission by Escames et al, aims to describe and clarify the proposed mechanism of action of melatonin inducing ROS production in cancer cells in order to propose future anti neoplastic clinical applications.
The manuscript is well written and organized, recent references are also included. I suggest considering the following reference: https://doi.org/10.1016/j.biochi.2022.05.008.
Therefore I suggest to publish in the present form after minor mispelling corrections.
- Corrected.
Page 9: Reference 95
Reviewer 2 Report
Dear authors, congratulations on the review article. After a careful reading, I have no objection to the publication as it stands.
My only suggestion is that the passage that begins at the top of page 8 ("It is also suggested...") be inserted in item 3.3, because there is a discussion about HIF there.
Author Response
First, we would like to return the revised manuscript entitled” “Understanding the mechanism of action of melatonin that induces ROS production in cancer cells” (Manuscript ID antioxidants-1864450) to the Journal of Antioxidants for the Special Issue*: Free-Radical Scavenging and Antioxidant Properties of Melatonin, for its consideration of publication. We would like to thank reviewers for critically examining this manuscript and providing us invaluable comments and suggestions. We respond to each of the reviewer’s comments and clarified/amended every necessary part. The statements addressing the referee's comments point by point are listed below and the revised text with alterations underlined and red font was uploaded. We highly appreciate your consideration and further process.
Amendments to the questions raised by the reviewer #2 to the manuscript ID antioxidants-1864450
Dear authors, congratulations on the review article. After a careful reading, I have no objection to the publication as it stands.
My only suggestion is that the passage that begins at the top of page 8 ("It is also suggested...") be inserted in item 3.3, because there is a discussion about HIF there.
- Corrected in page 5
