Silibinin Inhibits Cell Ferroptosis and Ferroptosis-Related Tissue Injuries
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. Cell Culture
2.3. Chemicals
2.4. Cell Viability Assay
2.5. Lipid Peroxidation Measurement
2.6. Labile Iron Pool Measurement
2.7. Prussian Blue Staining
2.8. 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) Assay
2.9. Kidney IRI
2.10. Hematoxylin–Eosin (H&E) Staining
2.11. Terminal Deoxynucleotidyl Transferase-Mediated dUTP Nick End Labeling (TUNEL) Staining
2.12. Q-PCR
2.13. Western Blot
2.14. Malondialdehyde (MDA) Assay
2.15. GSH Assay
2.16. Statistics
3. Results
3.1. Silibinin Inhibits Cell Ferroptosis
3.2. Silibinin Inhibits Ferroptosis through Antioxidant Activity
3.3. Silibinin Attenuates Ferroptosis-Induced Liver Injury in GPX4-Deficient Mice
3.4. Silibinin Attenuates Ferroptosis in the Kidney of GPX4-Deficient Mice
3.5. Silibinin Alleviates Renal Ischemia-Reperfusion Injury
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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The PCR Primers | Sequence | |
---|---|---|
Mouse-GPX4 | Forward | CCTCTGCTGCAAGAGCCTCCC |
Mouse-GPX4 | Reverse | CTTATCCAGGCAGACCATGTGC |
Mouse-ACSL4 | Forward | CCTTTGGCTCATGTGCTGGAAC |
Mouse-ACSL4 | Reverse | GCCATAAGTGTGGGTTTCAGTAC |
Mouse-PTGS2 | Forward | GCGACATACTCAAGCAGGAGCA |
Mouse-PTGS2 | Reverse | AGTGGTAACCGCTCAGGTGTTG |
Mouse-β-Actin | Forward | GGCTGTATTCCCCTCCATCG |
Mouse-β-Actin | Reverse | CCAGTTGGTAACAATGCCATGT |
Human-GPX4 | Forward | GAGGCAAGACCGAAGTAAACTAC |
Human-GPX4 | Reverse | CCGAACTGGTTACACGGGAA |
Human-CAT | Forward | TGGAGCTGGTAACCCAGTAGG |
Human-CAT | Reverse | CCTTTGCCTTGGAGTATTTGGTA |
Human-FSP1 | Forward | GATGAGCAACTTGGACAGCAA |
Human-FSP1 | Reverse | CTGGGCTGCTTATCTGGGAAG |
Human-GCH1 | Forward | GCCATGCAGTTCTTCACCAAGG |
Human-GCH1 | Reverse | ATGGAACCAAGTGATGCTCACAC |
Human-SLC7A11 | Forward | TCCTGCTTTGGCTCCATGAACG |
Human-SLC7A11 | Reverse | AGAGGAGTGTGCTTGCGGACAT |
Human-DHODH | Forward | GAGGACATTGCCAGTGTGGTCA |
Human-DHODH | Reverse | TTCCCACTCAGCCCTCCTGTTT |
Human-SOD1 | Forward | GGTGGGCCAAAGGATGAAGAG |
Human-SOD1 | Reverse | CCACAAGCCAAACGACTTCC |
Human-β-Actin | Forward | CATGTACGTTGCTATCCAGGC |
Human-β-Actin | Reverse | CTCCTTAATGTCACGCACGAT |
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Share and Cite
Duan, W.; Ou, Z.; Huang, Y.; Zhang, Y.; Zhang, L.; Zhao, Y.; He, R.; Zhang, Y.; Ge, Y.; Lou, H.; et al. Silibinin Inhibits Cell Ferroptosis and Ferroptosis-Related Tissue Injuries. Antioxidants 2023, 12, 2119. https://doi.org/10.3390/antiox12122119
Duan W, Ou Z, Huang Y, Zhang Y, Zhang L, Zhao Y, He R, Zhang Y, Ge Y, Lou H, et al. Silibinin Inhibits Cell Ferroptosis and Ferroptosis-Related Tissue Injuries. Antioxidants. 2023; 12(12):2119. https://doi.org/10.3390/antiox12122119
Chicago/Turabian StyleDuan, Wentao, Zexian Ou, Yuxing Huang, Yifan Zhang, Lan Zhang, Yanan Zhao, Ruikun He, Yihan Zhang, Yuanlong Ge, Huiling Lou, and et al. 2023. "Silibinin Inhibits Cell Ferroptosis and Ferroptosis-Related Tissue Injuries" Antioxidants 12, no. 12: 2119. https://doi.org/10.3390/antiox12122119