Marine-Derived Bioactive Peptides Self-Assembled Multifunctional Materials: Antioxidant and Wound Healing
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Randomized Self-Assembling Materials (CAPs)
2.3. Physical and Chemical Analysis of CAPs
2.4. Verification System of In Vitro Healing Activity
2.4.1. Test of Antioxidant Activity
2.4.2. The Test of In Vitro Plasma Recalcification Time
2.4.3. Cell Proliferation and Toxicity Test
2.5. Animal Grouping and Experiment
2.6. Healing Rate
2.7. Histology
2.8. Immunohistochemical Method
2.9. Sirius Red Picric Acid Staining
2.10. Molecular Docking
2.11. Data Statistics and Analysis
3. Results
3.1. Analysis of CAPs Characterization Results
3.2. Verification System of In Vitro Healing Activity
3.2.1. Test of Antioxidant Activity
3.2.2. The Test of In Vitro Plasma Recalcification Time
3.2.3. Cell Proliferation and Toxicity Test In Vitro
3.3. Effects of CAPs on Animal Experiment
3.3.1. The Effect on Wound Healing
3.3.2. Effect of CAPs on Histopathological Analysis of Traumatic Surfaces in Mice
3.3.3. Effect of CAPs on FGF and CD31 in Mice Wounds
3.3.4. Effect of CAPs on Collagen and Wound Healing in Mice
3.3.5. Biosafety Assessment of CAPs In Vivo
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sequence | Peptide Sequence of CAPs | Molecular Mass (Da) | Score |
---|---|---|---|
1 | Leu-Gln-Glu-Lys-Glu-Glu-Glu-Phe-Asp-Asn-Thr-Arg-Arg-Asn-His-Gln | 2071.967 | 52.05 |
2 | Leu-Asp-Val-Asn-His-Asp-Gly-Lys-Ile-Ser-Ile-Glu-Asp-Val-Glu-Glu-Ser-Arg-Asn-Lys | 2296.129 | 51.43 |
3 | Leu-Asp-Glu-Leu-Glu-Asp-Asn-Leu-Glu-Arg-Glu-Lys-Lys | 1629.821 | 48.84 |
4 | Ile-Gln-Asp-Lys-Glu-Gly-Ile-Pro-Pro-Asp-Gln-Gln-Arg | 1522.774 | 47.67 |
5 | Asp-Glu-Leu-Glu-Asp-Asn-Leu-Glu-Arg-Glu-Lys-Lys | 1516.737 | 45.66 |
6 | Leu-Gln-Glu-Lys-Glu-Glu-Glu-Phe-Asp-Asn-Thr-Arg-Arg | 1692.807 | 45.54 |
7 | Leu-Glu-Lys-Ser-Tyr-Glu-Leu-Pro-Asp-Gly-Gln-Val-Ile-Thr | 1590.814 | 45.34 |
8 | Ile-Glu-Glu-Asp-Ala-Gly-Leu-Gly-Asn-Gly-Gly-Leu-Gly-Arg | 1356.663 | 45.24 |
9 | Leu-Arg-Glu-Lys-Asp-Glu-Glu-Ile-Asp-Ser-Ile-Arg-Lys-Ser-Ser | 1803.933 | 44.6 |
10 | Ile-Ser-Ile-Glu-Asp-Val-Glu-Glu-Ser-Arg-Asn-Lys | 1417.705 | 44.16 |
11 | Leu-Asp-Glu-Leu-Glu-Asp-Asn-Leu-Glu-Arg-Glu-Lys | 1501.726 | 43.9 |
12 | Ala-Ala-Asp-Glu-Ser-Glu-Arg-Asn-Arg-Lys-Val | 1273.638 | 43.89 |
13 | Asp-Val-Asn-His-Asp-Gly-Lys-Ile-Ser-Ile-Glu | 1225.594 | 43.8 |
14 | Leu-Arg-Glu-Lys-Asp-Glu-Glu-Ile-Asp-Ser-Ile-Arg-Lys-Ser | 1716.901 | 43.56 |
15 | Glu-Lys-Ser-Tyr-Glu-Leu-Pro-Asp-Gly-Gln | 1164.53 | 43.46 |
16 | His-Gly-Asp-Ser-Asp-Leu-Gln-Leu-Glu-Arg | 1168.5472 | 43.42 |
17 | Leu-Glu-Lys-Ser-Tyr-Glu-Leu-Pro-Asp-Gly-Gln-Val | 1376.6824 | 43.15 |
18 | Glu-Lys-Ser-Tyr-Glu-Leu-Pro-Asp-Gly-Gln-Val-Ile | 1376.6824 | 43.05 |
19 | Leu-Asp-Val-Asn-His-Asp-Gly-Lys-Ile-Ser-Ile-Glu | 1338.6779 | 42.73 |
20 | Ile-Thr-Gly-Glu-Ser-Gly-Ala-Gly-Lys-Thr-Glu-Asn | 1162.5466 | 42.71 |
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Yu, D.; Cui, S.; Chen, L.; Zheng, S.; Zhao, D.; Yin, X.; Yang, F.; Chen, J. Marine-Derived Bioactive Peptides Self-Assembled Multifunctional Materials: Antioxidant and Wound Healing. Antioxidants 2023, 12, 1190. https://doi.org/10.3390/antiox12061190
Yu D, Cui S, Chen L, Zheng S, Zhao D, Yin X, Yang F, Chen J. Marine-Derived Bioactive Peptides Self-Assembled Multifunctional Materials: Antioxidant and Wound Healing. Antioxidants. 2023; 12(6):1190. https://doi.org/10.3390/antiox12061190
Chicago/Turabian StyleYu, Dingyi, Shenghao Cui, Liqi Chen, Shuang Zheng, Di Zhao, Xinyu Yin, Faming Yang, and Jingdi Chen. 2023. "Marine-Derived Bioactive Peptides Self-Assembled Multifunctional Materials: Antioxidant and Wound Healing" Antioxidants 12, no. 6: 1190. https://doi.org/10.3390/antiox12061190