Protection and Safety Evaluation of Live Constructions Derived from the Pgm− and pPCP1− Yersinia pestis Strain
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains, Plasmids, and Culture Conditions
2.2. Mice
2.3. Construction of Y. pestis Mutant Strains
2.4. Virulence Analysis in Mice
2.5. Determination of Protective Efficacy
2.6. Immune Responses
2.7. Evaluation of Vaccine Safety
2.8. Statistical Analysis
3. Results
3.1. Construction of Y. pestis Mutants with Lipid A Modification
3.2. Virulence of Y. pestis Mutants in Mice and Protective Immunity
3.3. Protection Efficiency Against Pulmonary Y. pestis Infection and Serum Immune Responses
3.4. Safety Assessment of Y. pestis Mutant Strains
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Financial Disclosure
Conflicts of Interest
References
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Strain | Relevant Genotype or Annotation | Source |
---|---|---|
E. coli TOP10 | F− mcrA ∆(mrr-hsdRMS-mcrBC) φ80lacZ∆M15 ∆lacX74 recA1 araD139 ∆(ara-leu)7697 galU galK rpsL endA1 nupG | Invitrogen |
Y. pestis KIM6+(pCD1Ap) | Pgm+, pMT1, pPCP1, pCD1Ap | (1) |
Y. pestis KIM10 | Pgm−, pMT1, pPCP1− | [33] |
YPS19 | ∆lpxP32::PlpxL lpxL Pgm− pPCP1− | This study |
YPS20 | ∆lpxP32::PlpxL lpxL ∆lacI23::Plpp lpxE Pgm− pPCP1− | This study |
KIM10(pCD1Ap) | pCD1Ap, pPCP1−, Pgm− | This study |
YPS19(pCD1Ap) | pCD1Ap, pPCP1−, Pgm−, ∆lpxP32::PlpxL lpxL | This study |
YPS20(pCD1Ap) | pCD1Ap, pPCP1−, Pgm−, ∆lpxP32::PlpxL lpxL ∆lacI23::Plpp lpxE | This study |
Plasmid | Source | |
pKD46 | λ Red recombinase expression plasmid | [34] |
pYA4373 | The cat-sacB cassette in the PstI and SacI sites of pUC18. | [35] |
pYA4577 | The PlpxLlpxL gene fragment flanked by the lpxP upstream and downstream sequence | [20] |
pYA4578 | The cat-sacB-PlpxLlpxL gene fragment flanked by the lpxP upstream and downstream sequence | [20] |
pYA4735 | The Plpp lpxE gene fragment flanked by the lacI upstream and downstream sequence | [21] |
pYA4736 | The cat-sacB-Plpp lpxE gene fragment flanked by the lacI upstream and downstream sequence | [21] |
Name | Sequence |
---|---|
LpxL1 | 5′gggagctccgctgatttgcgcgttaatgccctca3′ |
LpxL2 | 5′cggctgcaggaacataagaagaaaagataag3′ |
LpxE1 | 5′cgggagctcggataaccagaagcaataaaaaatc3′ |
LpxE2 | 5′cgggagctcctaaataatctcacgattacgca3′ |
Cm-V | 5′gttgtccatattggccacgttta3′ |
SacB-V | 5′gcagaagagatatttttaattgtgga3′ |
pPCP1−V1 | 5′cgggaattcagcaaaacagacaaacgcctgctgg3′ |
pPCP1−V2 | 5′cggctgcagtagacacccttaatctctctgcatg3′ |
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Wang, X.; Singh, A.K.; Sun, W. Protection and Safety Evaluation of Live Constructions Derived from the Pgm− and pPCP1− Yersinia pestis Strain. Vaccines 2020, 8, 95. https://doi.org/10.3390/vaccines8010095
Wang X, Singh AK, Sun W. Protection and Safety Evaluation of Live Constructions Derived from the Pgm− and pPCP1− Yersinia pestis Strain. Vaccines. 2020; 8(1):95. https://doi.org/10.3390/vaccines8010095
Chicago/Turabian StyleWang, Xiuran, Amit K. Singh, and Wei Sun. 2020. "Protection and Safety Evaluation of Live Constructions Derived from the Pgm− and pPCP1− Yersinia pestis Strain" Vaccines 8, no. 1: 95. https://doi.org/10.3390/vaccines8010095