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Volume 9
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Article

Evaluation of Recombinant Herpesvirus of Turkey Laryngotracheitis (rHVT-LT) Vaccine against Genotype VI Canadian Wild-Type Infectious Laryngotracheitis Virus (ILTV) Infection

by
Catalina Barboza-Solis
1,
Shahnas M. Najimudeen
1,
Ana Perez-Contreras
1,
Ahmed Ali
1,2,
Tomy Joseph
3,
Robin King
4,
Madhu Ravi
5,
Delores Peters
5,
Kevin Fonseca
6,
Carl A. Gagnon
7,
Frank van der Meer
1 and
Mohamed Faizal Abdul-Careem
1,*
1
Health Research Innovation Center 2C53, Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada
2
Department of Pathology, Beni-Suef University, Beni Suef 62511, Egypt
3
Animal Health Centre, Ministry of Agriculture, Food and Fisheries, Abbotsford, BC V3G 2M3, Canada
4
Agri Food Laboratories, Alberta Agriculture and Forestry, Edmonton, AB T6H 4P2, Canada
5
Animal Health and Assurance, Alberta Agriculture and Forestry, Edmonton, AB T6H 4P2, Canada
6
Provincial Laboratory for Public Health, Calgary, AB T2N 4W4, Canada
7
Swine and Poultry Infectious Diseases Research Center (CRIPA), Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte, Saint-Hyacinthe, QC J2S 2M2, Canada
*
Author to whom correspondence should be addressed.
Vaccines 2021, 9(12), 1425; https://doi.org/10.3390/vaccines9121425
Submission received: 6 November 2021 / Revised: 26 November 2021 / Accepted: 30 November 2021 / Published: 3 December 2021
(This article belongs to the Special Issue Poultry Vaccines)
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Abstract

In Alberta, infectious laryngotracheitis virus (ILTV) infection is endemic in backyard poultry flocks; however, outbreaks are only sporadically observed in commercial flocks. In addition to ILTV vaccine revertant strains, wild-type strains are among the most common causes of infectious laryngotracheitis (ILT). Given the surge in live attenuated vaccine-related outbreaks, the goal of this study was to assess the efficacy of a recombinant herpesvirus of turkey (rHVT-LT) vaccine against a genotype VI Canadian wild-type ILTV infection. One-day-old specific pathogen-free (SPF) White Leghorn chickens were vaccinated with the rHVT-LT vaccine or mock vaccinated. At three weeks of age, half of the vaccinated and the mock-vaccinated animals were challenged. Throughout the experiment, weights were recorded, and feather tips, cloacal and oropharyngeal swabs were collected for ILTV genome quantification. Blood was collected to isolate peripheral blood mononuclear cells (PBMC) and quantify CD4+ and CD8+ T cells. At 14 dpi, the chickens were euthanized, and respiratory tissues were collected to quantify genome loads and histological examination. Results showed that the vaccine failed to decrease the clinical signs at 6 days post-infection. However, it was able to significantly reduce ILTV shedding through the oropharyngeal route. Overall, rHVT-LT produced a partial protection against genotype VI ILTV infection.
Keywords: infectious laryngotracheitis virus; rHVT-LT recombinant vaccine; chicken; immune response infectious laryngotracheitis virus; rHVT-LT recombinant vaccine; chicken; immune response

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MDPI and ACS Style

Barboza-Solis, C.; Najimudeen, S.M.; Perez-Contreras, A.; Ali, A.; Joseph, T.; King, R.; Ravi, M.; Peters, D.; Fonseca, K.; Gagnon, C.A.; et al. Evaluation of Recombinant Herpesvirus of Turkey Laryngotracheitis (rHVT-LT) Vaccine against Genotype VI Canadian Wild-Type Infectious Laryngotracheitis Virus (ILTV) Infection. Vaccines 2021, 9, 1425. https://doi.org/10.3390/vaccines9121425

AMA Style

Barboza-Solis C, Najimudeen SM, Perez-Contreras A, Ali A, Joseph T, King R, Ravi M, Peters D, Fonseca K, Gagnon CA, et al. Evaluation of Recombinant Herpesvirus of Turkey Laryngotracheitis (rHVT-LT) Vaccine against Genotype VI Canadian Wild-Type Infectious Laryngotracheitis Virus (ILTV) Infection. Vaccines. 2021; 9(12):1425. https://doi.org/10.3390/vaccines9121425

Chicago/Turabian Style

Barboza-Solis, Catalina, Shahnas M. Najimudeen, Ana Perez-Contreras, Ahmed Ali, Tomy Joseph, Robin King, Madhu Ravi, Delores Peters, Kevin Fonseca, Carl A. Gagnon, and et al. 2021. "Evaluation of Recombinant Herpesvirus of Turkey Laryngotracheitis (rHVT-LT) Vaccine against Genotype VI Canadian Wild-Type Infectious Laryngotracheitis Virus (ILTV) Infection" Vaccines 9, no. 12: 1425. https://doi.org/10.3390/vaccines9121425

APA Style

Barboza-Solis, C., Najimudeen, S. M., Perez-Contreras, A., Ali, A., Joseph, T., King, R., Ravi, M., Peters, D., Fonseca, K., Gagnon, C. A., van der Meer, F., & Abdul-Careem, M. F. (2021). Evaluation of Recombinant Herpesvirus of Turkey Laryngotracheitis (rHVT-LT) Vaccine against Genotype VI Canadian Wild-Type Infectious Laryngotracheitis Virus (ILTV) Infection. Vaccines, 9(12), 1425. https://doi.org/10.3390/vaccines9121425

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