Meteorin Is a Novel Therapeutic Target for Wet Age-Related Macular Degeneration
Abstract
:1. Introduction
2. Material and Method
2.1. Ethics for Animal Use
2.2. Regulation of MTRN Expression in Rat Retina by Aldosterone and/or Spironolactone, Which Regulate the Mineralocorticoid Pathway
2.3. Immunohistochemistry on Rat Retina
2.4. Immunohistochemistry on Non-Human Primate and Human Samples
2.5. Laser-Induced CNV Rat Model
2.6. Treatments
2.7. Fluorescein Angiography (FA)
2.8. RPE/Choroid Flat-Mounts and CNV Quantifications
2.9. Statistics
2.10. RNA-Sequencing Data Analysis
3. Results
3.1. MTRN Is Widely Distributed in the Rat Neural Retina and in the Retinal Pigment Epithelium and Its Expression Is Regulated by Mineralocorticoid Pathway
3.2. MTRN Is Concentrated in the Macula and Co-Localized with Thrombospondin in Non-Human Primate
3.3. MTRN Is Distributed in All Retinal Layers of the Human Neural Retina
3.4. MTRN Is Secreted and Sequestered in PML Nuclear Bodies
3.5. MTRN Reduces Laser-Induced Choroidal Neovascularization Both in a Preventive and Curative Regimen
3.6. MTRN Regulates Genes and Pathways Involved in Angiogenesis Independent from VEGF in the RPE/Choroid and in the Retina
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Antibodies | Species | Reference | Lab provider | Dilution |
---|---|---|---|---|
Anti GFAP Antibody, Cy3 Conjugate | Mouse | MAB3402C3 | Dako cytomation | 1/200 |
Anti Meteorin | Rabbit | Ab12956 | Abcam | 1/100 |
Anti Glutamine synthetase clone GS-6 | Mouse | MAB 302 | Merck Millipore | 1/300 |
Anti TSP1 | Mouse | MA5-13398 | Invitrogen | 1/100 |
SUMO1 | Mouse | Sc5308 | Santa Cruz | 1/100 |
PML | Mouse | PG-M3 sc-966 | Santa Cruz | 1/100 |
AlexaFluo® 488 – AlexaFluo®594 – AlexaFluo®647 | Ab12956 | Invitrogen/Thermofischer | 1/300 | |
4′, 6′ –diamino-2-phenylindo DAPI | Sigma-Aldrich | 1/5000 |
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Delaunay, K.; Sellam, A.; Dinet, V.; Moulin, A.; Zhao, M.; Gelizé, E.; Canonica, J.; Naud, M.-C.; Crisanti-Lassiaz, P.; Behar-Cohen, F. Meteorin Is a Novel Therapeutic Target for Wet Age-Related Macular Degeneration. J. Clin. Med. 2021, 10, 2973. https://doi.org/10.3390/jcm10132973
Delaunay K, Sellam A, Dinet V, Moulin A, Zhao M, Gelizé E, Canonica J, Naud M-C, Crisanti-Lassiaz P, Behar-Cohen F. Meteorin Is a Novel Therapeutic Target for Wet Age-Related Macular Degeneration. Journal of Clinical Medicine. 2021; 10(13):2973. https://doi.org/10.3390/jcm10132973
Chicago/Turabian StyleDelaunay, Kimberley, Alexandre Sellam, Virginie Dinet, Alexandre Moulin, Min Zhao, Emmanuelle Gelizé, Jérémie Canonica, Marie-Christine Naud, Patricia Crisanti-Lassiaz, and Francine Behar-Cohen. 2021. "Meteorin Is a Novel Therapeutic Target for Wet Age-Related Macular Degeneration" Journal of Clinical Medicine 10, no. 13: 2973. https://doi.org/10.3390/jcm10132973