DNase-1 Treatment Exerts Protective Effects in Neurogenic Pulmonary Edema via Regulating the Neutrophil Extracellular Traps after Subarachnoid Hemorrhage in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Study Design
2.3. Drug Administration
2.4. Mice SAH Model
2.5. Mortality and Neurological Deficits
2.6. The NPE Criteria
2.7. Lung Histopathology and Immunofluorescent Staining
2.8. qRT-PCR
2.9. Flow Cytometric Analysis
2.10. Statistical Analysis
3. Results
3.1. DNase-1 Treatment Relieves the Injury of NPE after SAH
3.2. DNase-1 Treatment Inhibits NETs in NPE after SAH
3.3. DNase-1 Treatment Reduces the Proinflammatory Subtype Transition of Macrophage in NPE after SAH
3.4. NETs Are Possible Pathogenic Mechanism of NPE after SAH
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wu, X.; Guo, Y.; Zeng, H.; Chen, G. DNase-1 Treatment Exerts Protective Effects in Neurogenic Pulmonary Edema via Regulating the Neutrophil Extracellular Traps after Subarachnoid Hemorrhage in Mice. J. Clin. Med. 2022, 11, 4349. https://doi.org/10.3390/jcm11154349
Wu X, Guo Y, Zeng H, Chen G. DNase-1 Treatment Exerts Protective Effects in Neurogenic Pulmonary Edema via Regulating the Neutrophil Extracellular Traps after Subarachnoid Hemorrhage in Mice. Journal of Clinical Medicine. 2022; 11(15):4349. https://doi.org/10.3390/jcm11154349
Chicago/Turabian StyleWu, Xinyan, Yinghan Guo, Hanhai Zeng, and Gao Chen. 2022. "DNase-1 Treatment Exerts Protective Effects in Neurogenic Pulmonary Edema via Regulating the Neutrophil Extracellular Traps after Subarachnoid Hemorrhage in Mice" Journal of Clinical Medicine 11, no. 15: 4349. https://doi.org/10.3390/jcm11154349