1. Introduction
Gestational diabetes mellitus (GDM) is defined as any degree of hyperglycemia with the first onset during gestation [
1]. It occurs mainly during the second or third trimester of gestation. GDM affects approximately 7% of pregnancies worldwide [
2] and its incidence rate is predicted to increase in the near future [
2]. Two factors have been reported to promote impaired glucose control that ultimately leads to GDM onset: first, a reduced basal pancreatic islet cell function; second, the insulin resistance resulting from an increased maternal and placental hormonal production [
3].
Hyperglycemia in pregnancy is associated with a high risk of several adverse maternal, fetal, and neonatal outcomes [
4]. Adverse neonatal events related to GDM include macrosomia, hypoglycemia, jaundice, shoulder dystocia, and birth trauma. In addition, the offspring of women with GDM are more likely to develop insulin resistance, obesity, and type 2 diabetes over their lifetime [
5,
6,
7,
8]. Women with GDM are exposed to an increased risk of preeclampsia during gestation, and to increased risks of type 2 diabetes onset, metabolic syndrome, and cardiovascular disease after the pregnancy [
9].
Many different approaches have been proposed to screen and diagnose GDM [
4]. However, although GDM is one of the most prevalent pregnancy complications and represents a critical public health issue, there is currently no universal agreement over diagnostic methods. Since 2010, the IADPSG (International Association of Diabetes and Pregnancy Study Groups) diagnostic criteria have been applied almost worldwide [
10]. They are based on a universal screening with a 2 h 75 g Oral Glucose Tolerance Test (OGTT) performed between 24 and 28 weeks of gestation in all pregnant women without previous diabetes.
Recently, the validity of the OGTT as a gold-standard test for the diagnosis of GDM has been questioned due to the pre-analytical, analytical, and post-analytical variables potentially affecting its reproducibility and accuracy [
11]. Specifically, the analytical factors that could influence the OGTT results are its reproducibility (usually expressed as coefficient of variation) and bias (i.e., the difference from the true value, usually expressed as the percentage of the true value). To minimize these factors, a good laboratory test should conform with specific analytical regulatory criteria, as recommended by the National Academy of Clinical Biochemistry (NACB) [
12]. Particularly, for glucose measurement, the recommended targets are imprecision <2.9%, bias <2.2%, and total maximum allowable error <6.9%. Nevertheless, even within these targets there is no exact absolute estimate of the OGTT glucose levels and this theoretically influences GDM prevalence.
The aim of our study was to investigate the potential laboratory analytical issues in a large cohort of Caucasian women who underwent an OGTT for the diagnosis of GDM. Specifically, we wanted to explore the reliability of the OGTT by estimating GDM prevalence within the range of the total maximum allowable error.
2. Materials and Methods
This was an observational, retrospective, single-center study that was approved by the Local Ethics Committee of the University of Messina, Italy (protocol number 117/2012). All participants gave informed consent. Detailed methods of the women’s recruitment and the study procedures have been previously described [
13,
14]. All women underwent a 75 g OGTT for the diagnosis of GDM between 24 and 28 weeks of gestation. The OGTT results were interpreted according to the IADPSG diagnostic criteria [
11].
Women were advised not to exercise the day before the exam. The OGTT was performed at 8:00 a.m., after a 12 h overnight fast. A 3-day diet with a minimum of 150 g of carbohydrates per day before the OGTT was recommended, in accordance with the advice of the Fourth International Workshop-Conference on GDM [
15].
To minimize pre-analytical errors, we used citrate-buffered specimen tubes as recommended by the American Diabetes Association [
13]. To avoid glycolysis, we separated the plasma/serum within 30 min of sampling from blood cells prior to analysis. The plasma glucose was estimated by the hexokinase method (GLUC3, Cobas).
Women with a diagnosis of GDM were included in a specialist treatment plan with periodic visits until delivery. A personalized diet, a physical activity plan, a daily schedule of blood glucose and ketone checks, and eventual insulin therapy were prescribed.
Statistical Analyses
Data are reported as means and standard deviations for continuous variables and percentages for categorical variables.
We simulated different scenarios of GDM prevalence according to different possible types of analytical errors. First, we hypothesized a minimum error in the plasma glucose measurement consisting of a variation of 1 or 2 mg/dL more or less than the glucose value estimated by the laboratory for each OGTT point. To achieve this, we checked what would happen if only one of the OGTT points was affected by estimation error, assuming that the other two points were correctly estimated. For example, a scenario consisted of the OGTT baseline glucose value estimated by the laboratory plus 1 mg/dL and the 1 h and 2 h OGTT values as reported by the laboratory. Second, we explored the scenario of the total maximum allowable error by considering all three values of the OGTT estimated at the highest or the lowest possible value within the total maximum allowable error interval (i.e., baseline, 1 h, and 2 h OGTT glucose values all 6.9% higher or all 6.9% lower than laboratory estimates). The women’s baseline antenatal characteristics were reported according to the different scenarios. The level of agreement in GDM diagnoses (within any scenario) was evaluated by using the kappastatistic (k). The result is a coefficient with values less than or equal to 1, which can be expressed as a percentage. This agreement was graded as k = 0–19%, poor; 20–39%, fair; 40–59%, moderate; 60–79%, good; 80–100%, very good. A p-value <0.05 was considered for statistical significance. All the analyses were carried out using SPSS version 21 (SPSS, Inc., Chicago, IL, USA).
3. Results
Overall, 1015 women were evaluated, and following the IADPSG criteria, GDM was diagnosed in 12.2% (n = 124) of the cases.
If an error of glucose measurement occurred only for the OGTT baseline glucose value: 1 mg/dL and 2 mg/dL more than the OGTT fasting cutoff value would give a GDM prevalence of 12.1% (n = 123) and 11.2% (n = 114), respectively; 1 mg/dL and 2 mg/dL less than the cutoff value would give a GDM prevalence of 12.8% (n = 130) and of 13.6% (n = 138), respectively.
If an error of glucose measurement occurred only for the 1 h OGTT glucose value: 1 mg/dL and 2 mg/dL more than the 1 h OGTT cutoff value would give a GDM prevalence of 12.1% (n = 123) and 11.5% (n = 117), respectively; 1 mg/dL and 2 mg/dL less than the cutoff value would give a GDM prevalence of 12.3% (n = 125) and 12.7% (n = 129), respectively.
If an error of glucose measurement occurred only for the 2 h OGTT glucose value: 1 mg/dL and 2 mg/dL more than the 2 h OGTT cutoff value would give a GDM prevalence of 12.0% (n = 122) and 11.8% (n = 120), respectively; 1 mg/dL and 2 mg/dL less than the cutoff value would give a GDM prevalence of 12.7% (n = 129) and12.9% (n = 131), respectively.
Considering all OGTT glucose values estimated at the lowest or highest allowed value according to the total maximum allowable error, we would have a GDM prevalence of 4.5% (n = 46) and 25.3% (n = 257), respectively.
Baseline antenatal characteristics and risk factors for GDM in women according to the different scenarios are reported in
Table 1.
No significant difference between scenarios was detected for age, first trimester glucose values, parity, family history of diabetes, pre-pregnancy BMI, previous GDM, and previous macrosomia rate.
A moderate agreement was detected in the comparison of absoluteIADPSG thresholds for GDM diagnosis with lower (kappa 52.2%, p < 0.0001) and higher (kappa 58.1%, p < 0.0001) thresholds.
4. Discussion
Our study explored the analytical reliability of the OGTT in diagnosing GDM. The GDM prevalence significantly varied depending on the OGTT glucose level estimates at the lowest or highest allowed value according to the total maximum allowable error. Even a variation of 1 or 2 mg/dL more or less than the glucose value estimated by the laboratory for each OGTT point resulted in a significant change of the GDM prevalence. When comparing the IADPSG thresholds for GDM diagnosis with lower and higher thresholds of the total maximum allowable error, a moderate agreement was detected. Existing literature on this topic focused on the problem of reproducibility of the OGTT in pregnancy [
16,
17]. It is well known that, when repeated within two weeks in the same pregnant women, the OGTT does not give the same results. The main reasons for the low reliability of the OGTT can be divided into pre-analytical, analytical, and post-analytical issues. Pre-analytical issues include physical activity, gastric emptying [
18], stress and sleep [
19], and length of time spent in the fasting state. In order to minimize pre-analytical errors, we advised women to not exercise the day before the exam, to maintain a 12 h overnight fast, and to follow a 3 day diet with a minimum of 150 g of carbohydrate per day prior to the OGTT. All the analyses were performed by the same laboratory. We used citrate-buffered specimen tubes and, to avoid glycolysis, we separated plasma/serum within 30 min of sampling from blood cells prior to analysis. This potentially reduced the risk of errors in glucose measurement.
The results of our study are in line with those of Agarwal et al. [
20]. They tested the effect of laboratory analytical variation, assessed by the total analytical error of the three glucose OGTT cutoffs according to the criteria of the American Diabetes Association, the Canadian Diabetes Association, and the IADPSG. The authors concluded that, independent of the diagnostic criteria, any reported GDM prevalence can potentially vary between one-half and two times, even for laboratories meeting recommended quality specifications.
We did not have information regarding the potential impact of the different glucose tolerance classifications on neonatal outcomes, which is a major limitation of our study. However, a recent systematic review and meta-analysis showed that the increased risk of maternal outcomes (i.e., primary cesarean, induction of labor, maternal hemorrhage, and pregnancy-related hypertension) of women with GDM compared with women without GDM was not influenced by the GDM diagnostic classification [
21]. A second limitation is the lack of information on pregnant women from follow-ups after the pregnancy. In particular, we do not know if a correlation exists between the glucose status after pregnancy and the OGTT glucose values during pregnancy.
We enrolled a large number of women who were cared for by the same clinic. This prevented the occurrence of laboratory analytical heterogeneity. We followed a very specific protocol before and during the execution of the OGTT. This made it possible to minimize the risk of pre-analytical errors.
Our study has important implications for clinical practice. Health care professionals involved in the care of women with GDM should be wary of cases of GDM with a single OGTT value slightly lower or higher than the diagnostic cutoff. In the presence of an analytical error, a failure to diagnose GDM could occur with substantial possible repercussions on the treatment and on neonatal outcomes. Additionally, inappropriate diagnoses of GDM could occur in women with a normal glucose tolerance, resulting in medicalization and overtreatment of their pregnancies. A diagnostic strategy based on the assessment of the maternal risk factors associated with specific neonatal outcomes could overcome the diagnostic limitations of the OGTT. In this regard, emergent evidence seems to suggest that, according to the prenatal maternal characteristics, it is possible to classify subpopulations of women at greater risk of developing adverse neonatal outcomes [
22]. A history of previous macrosomia and the presence of pre-pregnancy obesity or overweight have been associated with the occurrence of specific neonatal adverse outcomes. Even when a risk stratification of adverse neonatal outcomes was performed by advanced statistical techniques, the analysis identified high-risk subgroups mainly characterized by high pre-pregnancy BMI. Our study did not find significant differences in the prevalence of these strong risk factors, even when different scenarios of errors in glucose measurement were hypothesized. This could mean that even in the presence of OGTT glucose values close to the GDM diagnostic cutoffs, a more complete assessment of adverse neonatal risk factors should be performed to follow-up with the women at higher risk.
Therefore, the diagnosis of GDM by glucose values gives a surrogate marker for real outcomes. The real outcomes such as fetal macrosomia or shoulder dystocia are relatively poorly predicted by the OGTT plasma glucose values.
The most relevant critical issue for the diagnosis of GDM remains the fact that, regardless of the screening modality (i.e., universal or risk-factors-based), it is based on a biochemical test that is spoiled by imprecision.
Additionally, it is important to examine the cost-effectiveness of an inaccurate diagnostic test. Analytical errors leading to an under- or overestimation of GDM prevalence could have a negative economic impact on public health. In fact, an overdiagnosis of GDM generates higher costs for the higher number of pregnant women involved in the care process. Indeed, in the case of GDM underdiagnosis, the costs could be generated by the higher number of newborns requiring intensive care or experiencing neonatal complications.
In conclusion, our results suggest that the OGTT’s current status as the gold standard method for diagnosing GDM deserves further consideration. A more accurate diagnostic approach based also on a complete evaluation of the risk factors associated with neonatal adverse outcomes is required. Women with OGTT glucose values closer to cutoff values require more attention in order to avoid clinical complications arising from GDM misclassification.
Author Contributions
Conceptualization, B.P. and A.D.B.; methodology, E.B. and G.D.V.; validation, F.C. (Francesco Corrado) and R.D.; formal analysis, B.P. and E.B.; investigation, G.D.V., R.D., F.C. (Francesca Chiereghin) and A.D.B.; writing—original draft preparation, B.P. and E.B.; writing—review and editing, all authors. All authors have read and agreed to the published version of the manuscript.
Funding
This research received no external funding.
Institutional Review and Board Statement
The study was conducted according to the guidelines of the declaration of Helsinki and approved by the Ethics Committee of the University of Messina (protocol number 117/2012).
Informed Consent Statement
Informed consent was obtained from all subjects involved in the study.
Data Availability Statement
Data are available from the corresponding author upon reasonable request.
Conflicts of Interest
The authors declare no conflict of interest.
References
- American Diabetes Association. Classification and diagnosis of diabetes: Standards of Medical Care in Diabetes—2021. Diabetes Care 2021, 44 (Suppl. 1), S15–S33. [Google Scholar] [CrossRef] [PubMed]
- Caissutti, C.; Berghella, V. Scientific Evidence for Different Options for GDM Screening and Management: Controversies and Review of the Literature. Biomed. Res. Int. 2017, 2017, 2746471. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Mackeen, A.D.; Lott, M. Gestational diabetes. In Maternal-Fetal Evidence Based Guidelines; Berghella, V., Ed.; CRC Press: Boca Raton, FL, USA, 2017; Chapter 3. [Google Scholar]
- Kuo, C.H.; Li, H.Y. Diagnostic Strategies for Gestational Diabetes Mellitus: Review of Current Evidence. Curr. Diabetes Rep. 2019, 19, 155. [Google Scholar] [CrossRef] [PubMed]
- Landon, M.B.; Spong, C.Y.; Thom, E.; Carpenter, M.W.; Ramin, S.M.; Casey, B.; Wapner, R.J.; Varner, M.W.; Rouse, D.J.; Thorp, J.M., Jr.; et al. A multicenter, randomized trial of treatment for mild gestational diabetes. N. Engl. J. Med. 2009, 361, 1339–1348. [Google Scholar] [CrossRef]
- Crowther, C.A.; Hiller, J.E.; Moss, J.R.; McPhee, A.J.; Jeffries, W.S.; Robinson, J.S. Australian Carbohydrate Intolerance Study in Pregnant Women [ACHOIS] Trial Group. Effect of treatment of gestational diabetes mellitus on pregnancy outcomes. N. Engl. J. Med. 2005, 352, 2477–2486. [Google Scholar] [CrossRef] [Green Version]
- Watson, D.; Rowan, J.; Neale, L.; Battin, M.R. Admissions to neonatal intensive care unit following pregnancies complicated by gestational or type 2 diabetes. Aust. N. Z. J. Obstet. Gynaecol. 2003, 43, 429–432. [Google Scholar] [CrossRef]
- Person, B.; Hanson, U. Neonatal morbidities in gestational diabetes mellitus. Diabetes Care 1998, 21 (Suppl. 2), B79–B84. [Google Scholar]
- Zhang, C.; Rawal, S.; Chong, Y.S. Risk factors for gestational diabetes: Is prevention possible? Diabetologia 2016, 59, 1385–1390. [Google Scholar] [CrossRef] [Green Version]
- International Association of Diabetes, Pregnancy Study Groups. International Association of Diabetes and Pregnancy Study Groups recommendations on the diagnosis and classification of hyperglycemia in pregnancy. Diabetes Care 2010, 33, 676–682. [Google Scholar] [CrossRef] [Green Version]
- Bogdanet, D.; O’Shea, P.; Lyons, C.; Shafat, A.; Dunne, F. The Oral Glucose Tolerance Test-Is It Time for a Change? A Literature Review with an Emphasis on Pregnancy. J. Clin. Med. 2020, 9, 3451. [Google Scholar] [CrossRef]
- Sacks, D.B.; Arnold, M.; Bakris, G.L.; Bruns, D.E.; Horvath, A.R.; Kirkman, M.S.; Lernmark, A.; Metger, B.E.; Nathan, D.M. Guidelines and recommendations for laboratory analysis in the diagnosis and management of diabetes mellitus. Clin. Chem. 2011, 57, e1–e47. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Corrado, F.; Pintaudi, B.; Di Vieste, G.; Interdonato, M.L.; Magliarditi, M.; Santamaria, A.; D’Anna, R.; Di Benedetto, A. Italian riskfactor-based screening for gestationaldiabetes. J. Matern-Fetal Neonatal Med. 2014, 27, 1445–1448. [Google Scholar] [CrossRef] [PubMed]
- Pintaudi, B.; Di Vieste, G.; Corrado, F.; Lucisano, G.; Pellegrini, F.; Giunta, L.; Nicolucci, A.; D’Anna, R.; Di Benedetto, A. Improvement of selective screening strategy for gestational diabetes through a more accurate definition of high-risk groups. Eur. J. Endocrinol. 2013, 170, 87–93. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Metzger, B.E.; Coustan, D.R. Summary and recommendations of the Fourth International Workshop-Conference on Gestational Diabetes Mellitus. The Organizing Committee. Diabetes Care 1998, 21 (Suppl. 2), B161–B167. [Google Scholar]
- Harlass, F.E.; Brady, K.; Read, J.A. Reproducibility of the oral glucose tolerance test in pregnancy. Am. J. Obstet. Gynecol. 1991, 64, 564–568. [Google Scholar] [CrossRef]
- Catalano, P.M.; Avallone, D.A.; Drago, N.M.; Amini, S.B. Reproducibility of the oral glucose tolerance test in pregnant women. Am. J. Obstet. Gynecol. 1993, 169, 874–881. [Google Scholar] [CrossRef]
- Jones, K.L.; Horowitz, M.; Wishart, M.J.; Maddox, A.F.; Harding, P.E.; Chatterton, B.E. Relationships between gastric emptying, intragastric meal distribution and blood glucose concentrations in diabetes mellitus. J. Nucl. Med. 1995, 36, 2220–2228. [Google Scholar]
- Reutrakul, S.; Zaidi, N.; Wroblewski, K.; Kay, H.H.; Ismail, M.; Ehrmann, D.A.; Van Cauter, E. Sleep Disturbances and Their Relationship to Glucose Tolerance in Pregnancy. Diabetes Care 2011, 34, 2454–2457. [Google Scholar] [CrossRef] [Green Version]
- Agarwal, M.M.; Dhatt, G.S.; Othman, Y. Gestational diabetes mellitus prevalence: Effect of the laboratory analytical variation. Diabetes Res. Clin. Pract. 2015, 109, 493–499. [Google Scholar] [CrossRef]
- Ramezani Tehrani, F.; Naz, M.S.G.; Yarandi, R.B.; Behboudi-Gandevani, S. The Impact of Diagnostic Criteria for Gestational Diabetes Mellitus on Adverse Maternal Outcomes: A Systematic Review and Meta-Analysis. J. Clin. Med. 2021, 10, 666. [Google Scholar] [CrossRef]
- Pintaudi, B.; Fresa, R.; Dalfrà, M.; Dodesini, A.R.; Vitacolonna, E.; Tumminia, A.; Sciacca, L.; Lencioni, C.; Marcone, T.; Lucisano, G.; et al. Strong Study Collaborators. The risk stratification of adverse neonatal outcomes in women with gestational diabetes [STRONG] study. Acta Diabetol. 2018, 55, 1261–1273. [Google Scholar] [CrossRef] [PubMed]
Table 1.
Risk factors for gestational diabetes according to different scenarios.
Table 1.
Risk factors for gestational diabetes according to different scenarios.
| OGTT Baseline Glucose Value | OGTT 1 h Glucose Value | OGTT 2 h Glucose Value | IADPSG Population |
---|
| ≥90 | ≥91 | ≥93 | ≥94 | ≥178 | ≥179 | ≥181 | ≥182 | ≥151 | ≥152 | ≥154 | ≥155 | |
Previous macrosomia (%) | 5.1 | 5.4 | 5.7 | 5.3 | 5.4 | 5.6 | 5.7 | 6.0 | 5.3 | 5.4 | 5.7 | 5.8 | 5.6 |
Previous GDM (%) | 7.2 | 7.7 | 8.1 | 8.8 | 7.8 | 8.0 | 8.1 | 8.5 | 7.6 | 7.8 | 6.6 | 6.7 | 8.1 |
Family history of diabetes (%) | 38.4 | 37.7 | 38.2 | 40.4 | 38.8 | 39.2 | 38.2 | 35.0 | 38.2 | 37.2 | 39.3 | 39.2 | 38.7 |
Parity > 1 (%) | 43.5 | 43.1 | 41.5 | 43.0 | 41.1 | 41.6 | 41.5 | 41.9 | 43.5 | 42.6 | 41.0 | 40.0 | 41.9 |
Pre-pregnancy BMI (kg/m2) | 25.5 ± 4.7 | 25.4 ± 4.7 | 25.3 ± 4.7 | 25.3 ± 4.7 | 25.2 ± 4.6 | 25.3 ± 4.7 | 25.3 ± 4.7 | 25.2 ± 4.5 | 25.1 ± 4.7 | 25.2 ± 4.7 | 25.4 ± 4.6 | 25.5 ± 4.7 | 25.3 ± 4.7 |
Pre-pregnancy BMI > = 25 (kg/m2) | 47.1 | 45.4 | 44.7 | 43.9 | 43.4 | 44.8 | 44.7 | 45.3 | 43.5 | 44.2 | 45.9 | 46.7 | 45.2 |
Age (years) | 32.0 ± 4.7 | 32.1 ± 4.8 | 32.0 ± 4.8 | 32.0 ± 4.8 | 32.0 ± 4.8 | 32.1 ± 4.8 | 32.0 ± 4.8 | 32.0 ± 4.9 | 31.9 ± 5.0 | 31.8 ± 5.0 | 32.0 ± 4.9 | 32.1 ± 4.8 | 32.0 ± 4.8 |
First trimester glucose value (mg/dL) | 86.0 ± 10.2 | 86.2 ± 10.0 | 86.2 ± 10.3 | 86.2 ± 10.3 | 86.2 ± 10.1 | 86.2 ± 10.2 | 86.1 ± 10.3 | 86.1 ± 10.1 | 86.0 ± 10.4 | 85.9 ± 10.4 | 86.2 ± 10.4 | 86.5 ± 10.2 | 86.2 ± 10.3 |
FPG values between 5.6 and 6.9 mmol/L (%) | 9.4 | 9.2 | 9.8 | 9.6 | 9.3 | 9.6 | 9.8 | 10.3 | 9.2 | 9.3 | 9.8 | 10.0 | 9.7 |
| Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).