Temporal Changes in the Skin Microbiome of Epidermolysis Bullosa Patients following the Application of Wound Dressings
Abstract
:1. Introduction
2. Patients and Methods
2.1. Study Design
2.2. Study Population
2.3. Sample Collection
2.4. DNA Extraction and 16S rRNA Gene Amplicon Sequencing
2.5. Data Processing and Analysis
3. Results
3.1. General Description of the Cohort
3.2. Taxonomic Abundance
3.3. Alpha Diversity
3.4. Beta Diversity
3.5. Differences in Microbiome Taxonomic Composition Per Sample Type
3.6. LefSE Biomarker-Based Differential Abundance between Sample Types
3.7. Taxonomic Abundance
3.8. Alpha Diversity
3.9. Beta Diversity
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Patient No. | Age (Years) | Sex | Ethnicity | EB Type | Diagnosis | Genetic Mutation |
---|---|---|---|---|---|---|
1 | 16 | Female | Jewish | Simplex localized | Clinical | N/A |
2 | 5 | Female | Bedouin | Simplex localized | Clinical | N/A |
3 | 7 | Male | Bedouin | Simplex localized | Clinical | N/A |
4 | 11 | Female | Bedouin | Simplex localized | Clinical | N/A |
5 | 9 | Male | Bedouin | Simplex localized | Clinical | N/A |
6 | 7 | Male | Bedouin | Simplex localized | Clinical | N/A |
7 | 9 | Female | Bedouin | Simplex localized | Clinical | N/A |
8 | 6 | Male | Bedouin | Simplex localized | Clinical | N/A |
9 | 3 | Male | Bedouin | Simplex localized | Clinical | N/A |
10 | 0.1 | Male | Bedouin | Simplex localized | Clinical | N/A |
11 | 2 | Male | Bedouin | Simplex localized | Clinical | N/A |
12 | 7 | Male | Bedouin | Simplex localized | Clinical | N/A |
13 | 14 | Male | Bedouin | Simplex localized | Clinical | N/A |
14 | 6 | Male | Bedouin | Simplex localized | Clinical | N/A |
15 | 5 | Male | Bedouin | Simplex localized | Clinical | N/A |
16 | 2 | Female | Bedouin | Simplex localized | Clinical | N/A |
17 | 2 | Female | Bedouin | Simplex localized | Clinical | N/A |
18 | 3 | Male | Bedouin | Simplex localized | Clinical | N/A |
19 | 5 | Female | Bedouin | Simplex localized | Clinical | N/A |
20 | 4 | Male | Bedouin | Simplex localized | Genetic | KRT14 |
21 | 1 | Female | Bedouin | Simplex generalized | Genetic | KRT14 |
22 | 13 | Male | Bedouin | Simplex generalized | Genetic | KRT14 |
23 | 0.9 | Male | Jewish | Simplex generalized | Genetic | KRT14 |
24 | 1 | Female | Bedouin | Simplex generalized | Genetic | KRT14 |
25 | 0.1 | Male | Bedouin | Simplex generalized | Genetic | KRT14 |
26 | 1 | Male | Bedouin | Simplex generalized | Genetic | KRT14 |
27 | 4 | Male | Bedouin | Simplex generalized | Genetic | PLEC |
28 | 1 | Female | Jewish | Simplex generalized | Genetic | PLEC |
29 | 0.5 | Male | Bedouin | Dystrophic recessive | Genetic | COL7A |
30 | 2 | Male | Bedouin | Dystrophic recessive | Genetic | COL7A |
31 | 2 | Male | Bedouin | Dystrophic recessive | Genetic | COL7A |
32 | 0.6 | Male | Bedouin | Dystrophic recessive | Genetic | COL7A |
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Horev, A.; Brandwein, M.; Vaknin, A.; Motro, Y.; Moran-Gilad, J. Temporal Changes in the Skin Microbiome of Epidermolysis Bullosa Patients following the Application of Wound Dressings. J. Clin. Med. 2023, 12, 6435. https://doi.org/10.3390/jcm12206435
Horev A, Brandwein M, Vaknin A, Motro Y, Moran-Gilad J. Temporal Changes in the Skin Microbiome of Epidermolysis Bullosa Patients following the Application of Wound Dressings. Journal of Clinical Medicine. 2023; 12(20):6435. https://doi.org/10.3390/jcm12206435
Chicago/Turabian StyleHorev, Amir, Michael Brandwein, Avraham Vaknin, Yair Motro, and Jacob Moran-Gilad. 2023. "Temporal Changes in the Skin Microbiome of Epidermolysis Bullosa Patients following the Application of Wound Dressings" Journal of Clinical Medicine 12, no. 20: 6435. https://doi.org/10.3390/jcm12206435