GLI1 Deficiency Impairs the Tendon–Bone Healing after Anterior Cruciate Ligament Reconstruction: In Vivo Study Using Gli1-Transgenic Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Study Design
2.3. Biomechanical Testing
2.4. Histologic Evaluation and Immunohistochemical (IHC) Staining
2.5. Micro-CT Examination
2.6. Statistical Analysis
3. Results
3.1. Biomechanical Evaluation
3.2. Histological Evaluation
3.3. Micro-CT
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Failure Mode | WT | GLI1-HET | GLI1-NULL |
---|---|---|---|
Pull out from femur | 5 | 6 | 6 |
Pull out from tibia | 1 | 0 | 1 |
Midsubstance failure | 1 | 1 | 0 |
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Liu, Y.; Liu, S.; Song, Z.; Chen, D.; Album, Z.; Green, S.; Deng, X.; Rodeo, S.A. GLI1 Deficiency Impairs the Tendon–Bone Healing after Anterior Cruciate Ligament Reconstruction: In Vivo Study Using Gli1-Transgenic Mice. J. Clin. Med. 2023, 12, 999. https://doi.org/10.3390/jcm12030999
Liu Y, Liu S, Song Z, Chen D, Album Z, Green S, Deng X, Rodeo SA. GLI1 Deficiency Impairs the Tendon–Bone Healing after Anterior Cruciate Ligament Reconstruction: In Vivo Study Using Gli1-Transgenic Mice. Journal of Clinical Medicine. 2023; 12(3):999. https://doi.org/10.3390/jcm12030999
Chicago/Turabian StyleLiu, Yake, Shaohua Liu, Zhe Song, Daoyun Chen, Zoe Album, Samuel Green, Xianghua Deng, and Scott A. Rodeo. 2023. "GLI1 Deficiency Impairs the Tendon–Bone Healing after Anterior Cruciate Ligament Reconstruction: In Vivo Study Using Gli1-Transgenic Mice" Journal of Clinical Medicine 12, no. 3: 999. https://doi.org/10.3390/jcm12030999