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Volume 13
Issue 5
10.3390/jcm13051240
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Article
Peer-Review Record

Digital Pathology Applications for PD-L1 Scoring in Head and Neck Squamous Cell Carcinoma: A Challenging Series

J. Clin. Med. 2024, 13(5), 1240; https://doi.org/10.3390/jcm13051240
by Valentina Canini 1, Albino Eccher 2,*, Giulia d’Amati 3, Nicola Fusco 4,5, Fausto Maffini 4, Daniela Lepanto 4, Maurizio Martini 6, Giorgio Cazzaniga 1, Panagiotis Paliogiannis 7, Renato Lobrano 7, Vincenzo L’Imperio 1 and Fabio Pagni 1
Reviewer 1:
Adel El-Naggar
Reviewer 2:
Mehdi Moghanibashi
J. Clin. Med. 2024, 13(5), 1240; https://doi.org/10.3390/jcm13051240
Submission received: 12 December 2023 / Revised: 29 January 2024 / Accepted: 20 February 2024 / Published: 22 February 2024
(This article belongs to the Special Issue Head and Neck Surgery: Clinical Updates on Diagnosis, Management and Prognosis)

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

The manuscript represents a study addressing the concordance between diagnostic and laboratory based assessment of PDL1 scoring.

The main issues are:

1.       The small sample size (11) of the cohort preclude comprehensive assessment meaningful statistical correlation and do not support any of the conclusions.

 

2.       The selection and application of computational programs are unclear and do not allow for meaningful interpretations of the results to clarify the clear explanation of inter-laboratory variabilities.

 

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

This study aimed to compare the the diagnostic performances between local pathologists  and a board of experts in the assessment of programmed death-ligand 1 (PD-L1) Combined Positive Scoring (CPS) on a challenging series of head and neck squamous cell carcinoma (HNSCC) through  a digital pathology platform. The authors have reported that discordances were noted in 5 (45%) cases, 2 (18%) due to abnormal nuclear/cytoplasmic localization of the LDT PD-L1 stain and 3 (27%) due to variation in terms of intensity, dirty background and diaminobenzidine (DAB) droplets.

 

Specific comments

 

1.     The number of samples used in this study was 11, which seems to be low from a statistical point of view. Was this number obtained by statistical methods?

2.     From the statistical tests, it seems that it has not been used. Are the obtained percentages statistically significant or not?

3.     The quality of the image 3 is not suitable. It is better to get a clearer figure.

 

4.     What is the difference between this study and the study of references 26 and 29?

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Round 2

Reviewer 2 Report

Comments and Suggestions for Authors

The authors addressed the all comments

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