Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
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- Sham-S group: A full layer colon resection was performed without creating ischemia to the rats, and end to tip anastomosis was applied without experimental drug administration. The rats were sacrificed at the end of 10 days.
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- Sham-I group: After ischemia was performed on the colon, a full layer colonic resection was performed and end to tip anastomosis was applied without experimental drug administration. The rats were sacrificed at the end of 10 days.
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- DXP group: After ischemia was performed on the colon, a full layer colonic resection was performed and end to tip anastomosis was applied. Dexpanthenol (Bepanthene 500 mg ampoule, Roche, Berlin, Germany) was administered at a dose of 50 mg/kg once a day for three days, intraperitoneally, and rats were sacrificed at the end of 10 days.
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- Q10 group: After ischemia was performed on the colon, a full layer colonic resection was performed and end to tip anastomosis was applied. CoQ10 (Sigma Chemical Co., St. Louis, MO, USA) was administered at a dose of 1 mg/100g once a day for three days, intraperitoneally, and rats were sacrificed at the end of 10 days.
2.2. Surgery
2.3. Burst Pressure Measurement
2.4. Biochemical Analysis
2.5. Histopathological Examination
2.6. Statistical Analysis
3. Results
3.1. Burst Pressure
3.2. Biochemical Analysis
3.3. Histopathological Evaluation
4. Discussion
5. Limitations
6. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Reepithelization of Anastomotic Mucosa (Components) | |
Grade 0 | No epithelization at anastomotic line |
Grade 1 | Incomplete coverage of the anastomotic line with a single cell layer |
Grade 2 | Complete coverage of the anastomotic line with a single cell layer |
Grade 3 | Complete reepithelization with glandular epithelium |
Neovascularization | |
Grade 1 | None |
Grade 2 | Minimal |
Grade 3 | Moderate |
Grade 4 | Severe |
Inflammation at Anastomotic Line (Polymorphonuclear Leukocyte or Lymphocyte) | |
Grade 1 | None |
Grade 2 | Minimal |
Grade 3 | Moderate |
Grade 4 | Severe |
Completeness of Muscular Layer | |
Grade 1 | Complete disruption |
Grade 2 | Incomplete disruption |
Grade 3 | Complete healing |
Group | Variable | Minimum | Maximum | Mean | SD |
---|---|---|---|---|---|
Sham-I | Burst pressure | 131.00 | 260.00 | 221.25 | 55.65 |
Tissue hydroxyproline level | 446.37 | 623.99 | 573.06 | 78.92 | |
Serum hydroxyproline level | 315.61 | 492.89 | 386.95 | 69.65 | |
Sham-S | Burst pressure | 250.00 | 305.00 | 267.00 | 17.67 |
Tissue hydroxyproline level | 485.12 | 1466.74 | 1027.66 | 328.06 | |
Serum hydroxyproline level | 386.27 | 892.65 | 747.96 | 161.27 | |
DXP | Burst pressure | 208.00 | 277.00 | 241.00 | 20.63 |
Tissue hydroxyproline level | 653.27 | 1434.89 | 1039.09 | 283.23 | |
Serum hydroxyproline level | 354.42 | 860.80 | 719.54 | 161.05 | |
Q10 | Burst pressure | 201.00 | 270.00 | 234.25 | 20.10 |
Tissue hydroxyproline level | 511.58 | 831.83 | 667.26 | 130.49 | |
Serum hydroxyproline level | 469.58 | 830.66 | 598.36 | 118.75 |
Variable | F | p |
---|---|---|
Burst pressure value | 6.797 | 0.008 |
Tissue hydroxyproline level | 8.855 | <0.001 |
Serum hydroxyproline level | 12.168 | <0.001 |
Group (I/J) | Burst Pressure | Tissue HP Level | Serum HP Level |
---|---|---|---|
Sham-I/Sham-S | 0.006 | 0.003 | <0.001 |
Sham-I/DXP | 0.016 | 0.002 | <0.001 |
Sham-I/Q10 | 0.005 | 0.844 | 0.018 |
Sham-S/DXP | 0.752 | 1.000 | 0.973 |
Sham-S/Q10 | 0.371 | 0.020 | 0.135 |
DXP/Q10 | 0.343 | 0.015 | 0.285 |
Group | Variable | Min | Max | Median | SD |
---|---|---|---|---|---|
Sham-I | Reepithelization | 0 | 3 | 3 | 1.41 |
Neovascularization | 3 | 4 | 4 | 0.35 | |
Polymorphonuclear leukocyte density | 3 | 4 | 3 | 0.35 | |
Lymphocyte density | 3 | 4 | 3 | 0.53 | |
Muscular layer completeness | 1 | 2 | 1 | 0.51 | |
Sham-S | Reepithelization | 3 | 3 | 3 | 0.00 |
Neovascularization | 2 | 4 | 3 | 0.75 | |
Polymorphonuclear leukocyte density | 2 | 3 | 2 | 0.46 | |
Lymphocyte density | 3 | 4 | 3 | 0.46 | |
Muscular layer completeness | 1 | 3 | 2.50 | 0.88 | |
DXP | Reepithelization | 0 | 3 | 1.50 | 1.60 |
Neovascularization | 2 | 4 | 4 | 0.74 | |
Polymorphonuclear leukocyte density | 1 | 3 | 3 | 0.75 | |
Lymphocyte density | 2 | 4 | 3 | 0.64 | |
Muscular layer completeness | 1 | 3 | 2 | 0.83 | |
Q10 | Reepithelization | 0 | 3 | 0 | 1.41 |
Neovascularization | 3 | 4 | 4 | 0.51 | |
Polymorphonuclear leukocyte density | 2 | 3 | 3 | 0.51 | |
Lymphocyte density | 3 | 4 | 4 | 0.51 | |
Muscular layer completeness | 1 | 3 | 1 | 0.91 |
Variable | X2 | p |
---|---|---|
Reepithelization | 9.192 | 0.027 |
Neovascularization | 7.577 | 0.056 |
Polymorphonuclear leukocyte density | 9.616 | 0.022 |
Lymphocyte density | 3.867 | 0.276 |
Muscular layer completeness | 5.618 | 0.132 |
Group | Reepithelization | PMNL |
---|---|---|
Sham-I/Sham-S | 0.064 | 0.003 |
ShamI/DXP | 0.473 | 0.045 |
Sham-I/Q10 | 0.135 | 0.044 |
Sham-S/DXP | 0.025 | 0.289 |
Sham-S/Q10 | 0.003 | 0.143 |
DXP/Q10 | 0.473 | 0.854 |
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Pehlivanlı, F.; Aydin, O.; Karaca, G.; Aydin, G.; Devrim, T.; Bulut, H.; Bakar, B.; Daphan, Ç.E. Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10. J. Clin. Med. 2018, 7, 161. https://doi.org/10.3390/jcm7070161
Pehlivanlı F, Aydin O, Karaca G, Aydin G, Devrim T, Bulut H, Bakar B, Daphan ÇE. Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10. Journal of Clinical Medicine. 2018; 7(7):161. https://doi.org/10.3390/jcm7070161
Chicago/Turabian StylePehlivanlı, Faruk, Oktay Aydin, Gökhan Karaca, Gülçin Aydin, Tuba Devrim, Huri Bulut, Bülent Bakar, and Çağatay Erden Daphan. 2018. "Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10" Journal of Clinical Medicine 7, no. 7: 161. https://doi.org/10.3390/jcm7070161