Salivary IL-6 mRNA is a Robust Biomarker in Oral Squamous Cell Carcinoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients and Control Subjects
2.2. Saliva Collection and Processing
2.3. Reverse Transcription and Real-Time Quantitative PCR (qPCR)
2.4. Enzyme-Linked Immune-Sorbent Assay (ELISA)
2.5. Immunohistochemistry (IHC)
2.6. Statistical Analysis
3. Results
3.1. Characteristics of Study Participants
3.2. Salivary IL-6 mRNA and IL-6 Protein Expression
3.3. IL-6 Protein Expression in OSCC Tumor Tissue
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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- | Patients (N = 95) | Controls (N = 80) | P | |
---|---|---|---|---|
Sex | Male | 60 (63%) | 30 (37%) | 0.004 * |
Female | 35 (37%) | 50 (63%) | ||
Age | (years; mean ± SD) | 61.7 ± 9.8 | 61.7 ± 9.2 | 0.982 ** |
OSCC stage | ||||
N = 70 | St I | 14 (20%) | – | na *** |
St II | 22 (31%) | – | na *** | |
St III | 13 (19%) | – | na*** | |
St IV | 21 (30%) | – | na *** | |
Histological grade | ||||
N = 70 | G1 | 17 (24%) | – | na *** |
G2 | 42 (60%) | – | na *** | |
G3 | 11 (16%) | – | na *** | |
DMFT | (N; mean ± SD) | 64; 27.6 ± 6.4 | 47; 24.8 ± 6.9 | 0.011 ** |
GI | (N; mean ± SD) | 36; 0.53 ± 0.49 | 41; 0.42 ± 0.34 | 0.79 ** |
Ethanol consumption | at least once a week | 46 (48%) | 14 (30%) | 0.035 * |
less than once a week | 49 (52%) | 33 (70%) | ||
Smoking | regular smoking | 62 (65%) | 8 (17%) | <0.001 * |
non-smoker or occasional smoker | 33 (35%) | 40 (83%) |
IL-6 mRNA Normalized Values | IL-6 Protein [pg/mL] | |||||||
---|---|---|---|---|---|---|---|---|
Sample | N | mean | SE | p * | N | mean | SE | p * |
OSCC | ||||||||
St I-II | 31 | 1.634 | 0.400 | <0.001 | 30 | 59.72 | 18.09 | <0.001 |
St III-IV | 23 | 23.694 | 19.850 | <0.001 | 28 | 102.44 | 28.22 | <0.001 |
G1 | 9 | 1.440 | 0.501 | <0.001 | 16 | 39.08 | 10.92 | 0.005 |
G2 | 34 | 16.327 | 13.550 | <0.001 | 35 | 102.56 | 26.24 | <0.001 |
G3 | 11 | 2.505 | 1.540 | <0.001 | 7 | 63.58 | 22.45 | 0.010 |
Total | 74 | 3.301 | 0.885 | <0.001 | 74 | 70.98 | 14.06 | <0.001 |
Control | 64 | 0.037 | 0.012 | 71 | 12.45 | 3.29 |
In Transformed IL-6 mRNA | In Transformed IL-6 Protein | ||||
---|---|---|---|---|---|
Adjusted linear regression coefficient | p-value | Adjusted linear regression coefficient | p-value | ||
Sex | female/male | −0.181 | 0.1 | −0.159 | 0.11 |
Age | Year | 0.234 | 0.037 | 0.174 | 0.094 |
DMFT | 0.234 | 0.037 | 0.174 | 0.094 | |
Gingival index | 0.094 | 0.488 | 0.201 | 0.1 | |
Alcohol consumption | |||||
at least once a | |||||
week/less than | 0.561 | 0.07 | −0.1 | 0.375 | |
once a week | |||||
Smoking | |||||
regular smoking | |||||
non-smoker or | −0.134 | 0.232 | 0.005 | 0.959 | |
Occasional | |||||
smoker |
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Márton, I.J.; Horváth, J.; Lábiscsák, P.; Márkus, B.; Dezső, B.; Szabó, A.; Tar, I.; Piffkó, J.; Jakus, P.; Barabás, J.; et al. Salivary IL-6 mRNA is a Robust Biomarker in Oral Squamous Cell Carcinoma. J. Clin. Med. 2019, 8, 1958. https://doi.org/10.3390/jcm8111958
Márton IJ, Horváth J, Lábiscsák P, Márkus B, Dezső B, Szabó A, Tar I, Piffkó J, Jakus P, Barabás J, et al. Salivary IL-6 mRNA is a Robust Biomarker in Oral Squamous Cell Carcinoma. Journal of Clinical Medicine. 2019; 8(11):1958. https://doi.org/10.3390/jcm8111958
Chicago/Turabian StyleMárton, Ildikó Judit, József Horváth, Péter Lábiscsák, Bernadett Márkus, Balázs Dezső, Adrienn Szabó, Ildikó Tar, József Piffkó, Petra Jakus, József Barabás, and et al. 2019. "Salivary IL-6 mRNA is a Robust Biomarker in Oral Squamous Cell Carcinoma" Journal of Clinical Medicine 8, no. 11: 1958. https://doi.org/10.3390/jcm8111958