Liquid Biopsy for the Detection of Resistance Mechanisms in NSCLC: Comparison of Different Blood Biomarkers
Abstract
:1. Introduction
2. Targeted Therapy in NSCLC
2.1. Anti-Epidermal Growth Factor Receptor
2.2. Anti-Anaplastic Lymphoma Kinase
2.3. Anti-ROS Proto-Oncogene 1
3. Immune Checkpoint Inhibitors
4. Liquid Biopsy in Monitoring Response to Treatment
4.1. Cell Free DNA
4.1.1. Cell Free DNA Biology
4.1.2. Clinical Application of cfDNA
4.2. Circulating Tumor Cells
4.2.1. Circulating Tumor Cell Biology
4.2.2. Potential Clinical Application of CTCs
4.3. Extracellular Vesicles
4.3.1. Extracellular Vesicle Biology
4.3.2. Potential Clinical Application of EVs
4.4. Circulating miRNA
4.4.1. Circulating miRNA Biology
4.4.2. Potential for Use of miRNAs in Clinical Practice
4.5. Platelets
4.5.1. Platelet Biology and Involvement in Cancer
4.5.2. Clinical Potentiality of Platelets in NSCLC
4.6. Circulating Proteins
5. Conclusions
Author Contributions
Conflicts of Interest
References
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Biomarkers | N Patients | Methodology | Sensitivity (%) | Specificity (%) | Reference |
---|---|---|---|---|---|
EGFR T790M | 23 | PNAClamp™ | 71.4 | 100 | Hur et al. [63] |
21 | SARMS | 39 | 100 | Maheswaran et al. [64] | |
208 | ddPCR™ | - | 57 | Jenkins et al. [65] | |
226 | cobas® | - | 51 | Jenkins et al. [65] | |
227 | NGS | - | 65 | Jenkins et al. [65] | |
216 | BEAMing | 69 | 70 | Oxnard et al. [66] | |
ALK resistance mutations | 7 | ddPCR™ | 2 mutations detected | - | Yoshida et al. [67] |
20 | ddPCR™ | 10 mutations detected | - | Bordi P et al. [68] | |
ROS1 resistance mutations | 18 | NGS | 6 mutations detected | - | Dagogo et al. [69] |
Biomarkers | N Patients | Methodology | Sensitivity (%) | Specificity (%) | Reference |
---|---|---|---|---|---|
EGFR T790M | 21 | SARMS | 94 | 100 | Maheswaran et al. [64] |
EML4-ALK | 39 | FISH | 100 | 100 | Pailler et al. [100] |
32 | FA-FISH | 100 | 100 | Pailler et al. [98] | |
87 | FISH | 100 | 100 | Ilie et al. [99] | |
PD-L1 expression | 71 | ICC | 55 | 100 | Ilie et al. [101] |
Biomarkers | N Patients | Methodology | Sensitivity (%) | Specificity (%) | Reference |
---|---|---|---|---|---|
EGFR T790M | 23 | PNAClamp™ | 100 | 100 | Hur et al. [63] |
PD-L1 expression | 8 | ddPCR™ | - | - | Del Re et al. [132] |
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Pasini, L.; Ulivi, P. Liquid Biopsy for the Detection of Resistance Mechanisms in NSCLC: Comparison of Different Blood Biomarkers. J. Clin. Med. 2019, 8, 998. https://doi.org/10.3390/jcm8070998
Pasini L, Ulivi P. Liquid Biopsy for the Detection of Resistance Mechanisms in NSCLC: Comparison of Different Blood Biomarkers. Journal of Clinical Medicine. 2019; 8(7):998. https://doi.org/10.3390/jcm8070998
Chicago/Turabian StylePasini, Luigi, and Paola Ulivi. 2019. "Liquid Biopsy for the Detection of Resistance Mechanisms in NSCLC: Comparison of Different Blood Biomarkers" Journal of Clinical Medicine 8, no. 7: 998. https://doi.org/10.3390/jcm8070998