Coagulation Changes during Central Hypovolemia across Seasons
Abstract
:1. Introduction
2. Experimental Section
2.1. Subjects
2.2. Sample Size
2.3. Study and LBNP Protocol
2.4. Blood Sampling
2.5. Statistics
3. Results
3.1. LBNP Effects on Standard Coagulation Parameters
3.2. LBNP Effects on Blood Cell Count, Haematocrit, Haemoglobin, MCHC, MCH and MCV
3.3. LBNP Effects on Thrombin Generation Markers
3.4. Thrombin Generation Using CAT and TEM
3.5. CAT Measurements during LBNP
3.6. TEM Measurements during LBNP
3.7. Endothelial Activation during LBNP
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
aPTT | activated partial thromboplastin time |
CAT | calibrated automated thrombography |
CFT | clot formation time |
CT | coagulation time |
ETP | endogenous thrombin potential |
F1+2 | prothrombin fragment 1+2 |
Hct | Haematocrit |
Hb | Haemoglobin |
LBNP | lower body negative pressure |
LT | Lag time |
MCF | maximum clot firmness |
MCH | mean corpuscular haemoglobin |
MCHC | mean corpuscular haemoglobin concentration |
MCV | mean corpuscular volume |
OC | oral contraceptives |
PC | protein C |
Plt | platelet count |
PS | protein S |
PT | prothrombin times |
PPP | platelet poor plasma |
RBC | red blood cell counts |
SD | standard deviation |
TAT | thrombin/antithrombin complex |
TEM | thrombelastometry |
TF | tissue factor |
t-PA | tissue-plasminogen activator |
VTE | venous thromboembolism |
WB | whole blood |
WBC | white blood cell counts. |
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Season | Variable | Baseline | LBNP | Recovery | p-Value (ANOVA) |
---|---|---|---|---|---|
Summer | aPTT (s) | 34.67 ± 2.78 | 32.30 ± 3.09 | 33.25 ± 4.47 | 0.018 |
PT (%) | 103.92 ± 13.10 | 107.23 ± 13.73 | 104.08 ± 15.31 | 0.17 | |
FII (%) | 103.31 ± 15.72 | 102.23 ± 17.14 | 103.67 ± 15.50 | 0.91 | |
FVII (%) | 105.77 ± 35.15 | 107.85 ± 36.79 | 107.42 ± 37.44 | 0.61 | |
F VIII (%) | 105.00 ± 36.63 | 122.62 ± 55.92 | 126.42 ± 64.26 | 0.021 | |
PC (%) | 97.23 ± 31.31 | 101.00 ± 27.83 | 98.50 ± 31.77 | 0.56 | |
PS (%) | 85.85 ± 16.26 | 85.15 ± 22.70 | 91.92 ± 20.88 | 0.35 | |
WBC (103/µL) | 5.11 ± 0.98 | 4.78 ± 1.11 | 5.43 ± 1.40 | 0.0001 | |
Plt (103/mL) | 203.15 ± 55.14 | 198.40 ± 54.00 | 208.58 ± 56.35 | 0.063 | |
RBC (106/mL) | 4.33 ± 0.40 | 4.26 ± 0.40 | 4.38 ± 0.40 | 0.0001 | |
Hct (%) | 37.12 ± 3.31 | 36.52 ± 3.36 | 37.44 ± 3.31 | <0.0001 | |
Hb (g/dL) | 12.72 ± 1.38 | 12.58 ± 1.39 | 12.89 ± 1.46 | <0.0001 | |
MCHC (g/dL) | 34.24 ± 1.12 | 34.40 ± 1.17 | 34.36 ± 1.24 | 0.35 | |
MCH (pg) | 29.37 ± 1.38 | 29.53 ± 1.33 | 29.38 ± 1.47 | 0.26 | |
MCV (fL) | 85.78 ± 2.77 | 85.77 ± 2.69 | 85.50 ± 2.70 | 0.94 | |
F1+2 (pmol/L) | 263.7 ± 142.6 | 776.3 ± 567.3 | 1078.4 ± 837.4 | 0.0027 | |
TAT (ng/mL) | 7.26 ± 13.36 | 37.21 ± 23.23 | 42.02 ± 23.62 | 0.0004 | |
TF (pg/mL) | 583.3 ± 241.9 | 593.7 ± 314.2 | 575.3 ± 169.0 | 0.63 | |
tPA (ng/mL) | 2.12 ± 1.96 | 1.45 ± 1.09 | 1.58 ± 0.85 | 0.25 | |
LT (min) | 2.36 ± 0.30 | 2.31 ± 0.24 | 2.46 ± 0.27 | 0.028 | |
ETP (nM min) | 1487.5 ± 449.3 | 1529.0 ± 417.3 | 1501.9 ± 484.7 | 0.20 | |
Peak (nM) | 273.30 ± 94.39 | 287.03 ± 77.09 | 263.55 ± 101.01 | 0.014 | |
ttPeak (min) | 5.61 ± 0.83 | 5.38 ± 0.57 | 5.92 ± 0.97 | 0.0025 | |
VelIndex (nm/min) | 91.54 ± 43.94 | 97.38 ± 35.92 | 85.53 ± 46.76 | 0.036 | |
StartTail (min) | 21.18 ± 1.73 | 21.29 ± 1.50 | 21.82 ± 1.79 | 0.27 | |
CT (s) | 199.38 ± 46.33 | 172.92 ± 33.57 | 188.83 ± 32.53 | 0.023 | |
CFT (s) | 151.23 ± 74.70 | 121.23 ± 38.37 | 142.67 ± 59.64 | 0.27 | |
MCF (mm) | 56.77 ± 6.73 | 58.00 ± 5.72 | 56.67 ± 5.93 | 0.49 | |
Alpha (°) | 63.00 ± 9.58 | 66.69 ± 6.19 | 64.17 ± 8.91 | 0.27 | |
Winter | aPTT (s) | 35.62 ± 6.05 | 33.69 ± 4.62 | 36.98 ± 16.16 | 0.71 |
PT (%) | 103.85 ± 11.86 | 101.62 ± 10.88 | 106.00 ± 11.18 | 0.028 | |
FII (%) | 85.69 ± 10.65 | 84.25 ± 7.52 | 85.17 ± 5.26 | 0.46 | |
FVII (%) | 107.62 ± 22.75 | 108.17 ± 23.30 | 109.00 ± 24.17 | 0.69 | |
F VIII (%) | 107.77 ± 45.85 | 119.58 ± 52.92 | 124.75 ± 54.96 | 0.0053 | |
PC (%) | 94.15 ± 17.42 | 95.58 ± 15.11 | 95.08 ± 19.41 | 0.98 | |
PS (%) | 83.23 ± 23.48 | 88.00 ± 25.73 | 83.33 ± 16.04 | 0.61 | |
WBC (103/µL) | 4.76 ± 0.77 | 4.23 ± 0.59 | 4.93 ± 0.79 | <0.0001 | |
Plt (103/mL) | 192.54 ± 42.19 | 195.08 ± 38.50 | 195.62 ± 36.90 | 0.64 | |
RBC (106/mL) | 4.27 ± 0.33 | 4.25 ± 0.37 | 4.33 ± 0.35 | 0.0036 | |
Hct (%) | 36.72 ± 2.56 | 36.54 ± 2.91 | 37.26 ± 2.64 | 0.0039 | |
Hb (g/dL) | 12.69 ± 1.07 | 12.64 ± 1.22 | 12.89 ± 1.15 | 0.0088 | |
MCHC (g/dL) | 34.54 ± 1.01 | 34.54 ± 1.04 | 34.56 ± 1.00 | 0.97 | |
MCH (pg) | 29.73 ± 0.99 | 29.74 ± 0.97 | 29.76 ± 1.00 | 0.91 | |
MCV (fL) | 86.09 ± 2.43 | 86.10 ± 2.33 | 86.12 ± 2.27 | 0.98 | |
F1+2 (pmol/L) | 212.3 ± 27.80 | 546.2 ± 99.37 | 584.3 ± 108.7 | 0.036 | |
TAT (ng/mL) | 3.30 ± 0.32 | 41.77 ± 8.81 | 41.19 ± 9.96 | 0.004 | |
TF (pg/mL) | 351.1 ± 165.9 | 324.2 ± 170.4 | 377.92 ± 137.9 | 0.49 | |
tPA (ng/mL) | 1.88 ± 1.66 | 1.71 ± 0.99 | 1.59 ± 0.85 | 0.8 | |
LT (min) | 2.13 ± 0.10 | 2.22 ± 0.11 | 2.27 ± 0.14 | 0.184 | |
ETP (nM min) | 1688 ± 214 | 1645 ± 210 | 1645 ± 197 | 0.347 | |
Peak (nM) | 312.80 ± 43.15 | 304.50 ± 42.68 | 297.30 ± 38.44 | 0.315 | |
ttPeak (min) | 5.10 ± 0.31 | 5.19 ± 0.26 | 5.35 ± 0.28 | 0.239 | |
VelIndex (nm/min) | 116.60 ± 24.64 | 110.90 ± 21.30 | 103.20 ± 19.57 | 0.079 | |
StartTail (min) | 21.46 ± 0.43 | 21.55 ± 0.39 | 21.44 ± 0.41 | 0.906 | |
CT (s) | 155.60 ± 13.11 | 144.40 ± 13.00 | 148.40 ± 13.48 | 0.076 | |
CFT (s) | 121.70 ± 15.53 | 107.30 ± 20.56 | 99.86 ± 11.04 | 0.122 | |
MCF (mm) | 60.86 ± 2.06 | 62.00 ± 1.98 | 62.00 ± 2.01 | 0.500 | |
Alpha (°) | 66.14 ± 2.49 | 69.57 ± 3.12 | 70.00 ± 2.05 | 0.035 |
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Goswami, N.; Taucher, A.A.; Brix, B.; Roessler, A.; Koestenberger, M.; Reibnegger, G.; Cvirn, G. Coagulation Changes during Central Hypovolemia across Seasons. J. Clin. Med. 2020, 9, 3461. https://doi.org/10.3390/jcm9113461
Goswami N, Taucher AA, Brix B, Roessler A, Koestenberger M, Reibnegger G, Cvirn G. Coagulation Changes during Central Hypovolemia across Seasons. Journal of Clinical Medicine. 2020; 9(11):3461. https://doi.org/10.3390/jcm9113461
Chicago/Turabian StyleGoswami, Nandu, Alexander Andreas Taucher, Bianca Brix, Andreas Roessler, Martin Koestenberger, Gilbert Reibnegger, and Gerhard Cvirn. 2020. "Coagulation Changes during Central Hypovolemia across Seasons" Journal of Clinical Medicine 9, no. 11: 3461. https://doi.org/10.3390/jcm9113461