An Outbreak of Clostridium (Clostridioides) difficile Infections within an Acute and Long-Term Care Wards Due to Moxifloxacin-Resistant PCR Ribotype 176 Genotyped as PCR Ribotype 027 by a Commercial Assay
Abstract
:1. Introduction
2. Material and Methods
2.1. Diagnostics of CDI
2.2. Molecular Typing of Isolates
2.3. Antimicrobial Susceptibility Testing
3. Results
3.1. Presumptive Ribotype 027 CDI Outbreak Was Identified from Stool Samples
3.2. Suspected C. difficile RT027 in Stools Were Identified as C. difficile RT176 at a Strain Level
3.3. Moxifloxacin Resistance Due to Thr82Ile Was Identified in Majority of Isolates
3.4. Clonal Relatedness of RT176 Isolates Were Identified from Samples within Acute and Long-Term Care Wards
3.5. RT176 Infection Is Not Associated with Fatal Outcome or Recurrence of CDI
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Novakova, E.; Kotlebova, N.; Gryndlerova, A.; Novak, M.; Vladarova, M.; Wilcox, M.; Kuijper, E.; Krutova, M. An Outbreak of Clostridium (Clostridioides) difficile Infections within an Acute and Long-Term Care Wards Due to Moxifloxacin-Resistant PCR Ribotype 176 Genotyped as PCR Ribotype 027 by a Commercial Assay. J. Clin. Med. 2020, 9, 3738. https://doi.org/10.3390/jcm9113738
Novakova E, Kotlebova N, Gryndlerova A, Novak M, Vladarova M, Wilcox M, Kuijper E, Krutova M. An Outbreak of Clostridium (Clostridioides) difficile Infections within an Acute and Long-Term Care Wards Due to Moxifloxacin-Resistant PCR Ribotype 176 Genotyped as PCR Ribotype 027 by a Commercial Assay. Journal of Clinical Medicine. 2020; 9(11):3738. https://doi.org/10.3390/jcm9113738
Chicago/Turabian StyleNovakova, Elena, Nina Kotlebova, Anezka Gryndlerova, Martin Novak, Michala Vladarova, Mark Wilcox, Ed Kuijper, and Marcela Krutova. 2020. "An Outbreak of Clostridium (Clostridioides) difficile Infections within an Acute and Long-Term Care Wards Due to Moxifloxacin-Resistant PCR Ribotype 176 Genotyped as PCR Ribotype 027 by a Commercial Assay" Journal of Clinical Medicine 9, no. 11: 3738. https://doi.org/10.3390/jcm9113738