OVOL2-Mediated ZEB1 Downregulation May Prevent Promotion of Actinic Keratosis to Cutaneous Squamous Cell Carcinoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Tissue Samples
2.2. Immunohistochemical Analysis
2.3. Cell Culture
2.4. A Gene-Specific Small Interfering RNA (siRNA) Transfection
2.5. Quantitative Reverse-Transcription Polymerase Chain Reaction (qRT-PCR) Analysis
2.6. Western Blotting
2.7. Matrigel Invasion Chamber Assay
2.8. Wound Healing Assay
2.9. Proliferation Assay
2.10. Colony Formation Assay
2.11. Apoptosis Assay
2.12. Statistical Analysis
3. Results
3.1. Immunohistochemistry
3.1.1. OVOL1/2 Expression in AK and cSCC
3.1.2. ZEB1 and Vimentin Expression in AK and cSCC
3.1.3. Associations between OVOL1/2 and ZEB1 or Vimentin in AK and cSCC
3.2. In Vitro Assays Using an A431 Cell Line
3.2.1. Screening of EMT-Related Factors Influenced by OVOL1/2 Knockdown
3.2.2. Impacts of OVOL1/2 Knockdown on Vimentin, ZEB1, and E-cadherin Protein Levels
3.2.3. Increased Invasion upon OVOL2 Knockdown
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Total | OVOL1 | p-Value | ||
---|---|---|---|---|
High | Low | |||
ZEB1 | 0.245 | |||
Positive | 19 | 10 | 9 | |
Negative | 41 | 29 | 12 | |
Total | OVOL2 | p-value | ||
High | Low | |||
ZEB1 | 0.0105* | |||
Positive | 19 | 7 | 12 | |
Negative | 41 | 30 | 11 |
Total | OVOL1 | p-Value | ||
---|---|---|---|---|
High | Low | |||
Vimentin | 0.352 | |||
Positive | 15 | 8 | 7 | |
Negative | 45 | 31 | 14 | |
Total | OVOL2 | p-value | ||
High | Low | |||
Vimentin | 0.543 | |||
Positive | 15 | 8 | 7 | |
Negative | 45 | 29 | 16 |
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Murata, M.; Ito, T.; Tanaka, Y.; Yamamura, K.; Furue, K.; Furue, M. OVOL2-Mediated ZEB1 Downregulation May Prevent Promotion of Actinic Keratosis to Cutaneous Squamous Cell Carcinoma. J. Clin. Med. 2020, 9, 618. https://doi.org/10.3390/jcm9030618
Murata M, Ito T, Tanaka Y, Yamamura K, Furue K, Furue M. OVOL2-Mediated ZEB1 Downregulation May Prevent Promotion of Actinic Keratosis to Cutaneous Squamous Cell Carcinoma. Journal of Clinical Medicine. 2020; 9(3):618. https://doi.org/10.3390/jcm9030618
Chicago/Turabian StyleMurata, Maho, Takamichi Ito, Yuka Tanaka, Kazuhiko Yamamura, Kazuhisa Furue, and Masutaka Furue. 2020. "OVOL2-Mediated ZEB1 Downregulation May Prevent Promotion of Actinic Keratosis to Cutaneous Squamous Cell Carcinoma" Journal of Clinical Medicine 9, no. 3: 618. https://doi.org/10.3390/jcm9030618