Transient Existence of Circulating Mesenchymal Stem Cells in the Deep Veins in Humans Following Long Bone Intramedullary Reaming
Abstract
:1. Introduction
2. Materials and Methods
2.1. Donors and Tissue Collection
2.2. Preparation of Tissue and Generation of MSC Cultures
2.3. Colony-Forming Unit-Fibroblast Assay
2.4. Phenotypic Analysis of Putative Blood-Derived MSCs
2.5. Osteogenic Differentiation Assay
2.6. Gene Expression of MSCs
2.7. Hierarchical Clustering
2.8. Statistical Analysis
3. Results
3.1. Identification and Frequency of MSC-Like Colonies
3.2. Circulatory CFU-Fs in Femoral and Peripheral Veins.
3.3. Molecular Profile of Blood-Derived CFU-Fs.
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Group | Surgery/Sampling Time | Bloods | Total Blood Volumes (mL) | n | F:M | Median Age (Range) |
---|---|---|---|---|---|---|
Reaming | Patients undergoing reaming | FV+PV | 510 + 510 | 24 | 10:14 | 44.5 (18–66) |
a) Sequential conventional reaming for IM nailing of closed tibial shaft fracture. | FV+PV | 360 + 360 | 9 | 3:6 | 40 (18–53) | |
b) All fracture non-union patients requiring RIA for bone graft acquisition. | FV+PV | 150 + 150 | 15 | 7:8 | 45 (19–66) | |
Prior surgery | Current surgery not relevant #, samples taken before surgical intervention | FV+PV | 120 + 120 | 12 | 5:7 | 40 (18–67) |
Early RA | N/A | PV | 168 | 15 | 11:4 | 51 (23–77) |
Established RA | N/A | PV | 168 | 11 | 7:4 | 47 (32–66) |
HC | N/A | PV | 120 | 12 | 4:8 | 33.5 (24–54) |
OVERALL | FV + PV | 630 + 1086 | 74 | 37:37 | 43.5 (18–77) |
ICBM-MSCs | LBM-MSCs | CB-MSCs | Peri-MSCs | A-MSCs | ||||||
---|---|---|---|---|---|---|---|---|---|---|
Comparator | BL-MSCs | FVB- MSCs | BL-MSCs | FVB- MSCs | BL-MSCs | FVB- MSCs | BL-MSCs | FVB- MSCs | BL-MSCs | FVB-MSCs |
ACTA2 | 0.028 | |||||||||
ACVR1B | 0.005 | 0.009 | ||||||||
ACVR2A | 0.010 | 0.032 | <0.001 | 0.002 | ||||||
BGLAP | 0.006 | 0.008 | 0.010 | 0.014 | ||||||
BMP2 | 0.012 | 0.042 | ||||||||
CEBPA | 0.027 | <0.001 | 0.002 | 0.018 | 0.001 | 0.004 | ||||
COL1A2 | 0.021 | |||||||||
CXCL12 | 0.013 | |||||||||
DAAM2 | 0.016 | |||||||||
DVL2 | 0.041 | 0.003 | 0.011 | |||||||
EFNA1 | 0.040 | |||||||||
FABP4 | 0.029 | 0.008 | 0.038 | 0.008 | 0.020 | |||||
FZD9 | 0.038 | 0.008 | ||||||||
GJA1 | 0.037 | 0.042 | ||||||||
IGF2 | 0.048 | |||||||||
MCAM | 0.038 | |||||||||
MSX1 | 0.003 | 0.030 | 0.050 | |||||||
NOG | 0.014 | |||||||||
PPARG | 0.043 | 0.041 | ||||||||
RUNX2 | 0.017 | |||||||||
SORT1 | 0.006 | |||||||||
SP7 | 0.008 | |||||||||
SPP1 | 0.017 | 0.041 | <0.001 | 0.038 | ||||||
TGFBR3 | 0.015 | <0.001 | 0.001 | |||||||
Differences (BL-MSCs) | 8 | 9 | 9 | 5 | 8 | |||||
ANGPTL4 | 0.012 | |||||||||
BMPER | 0.021 | 0.031 | ||||||||
DDR2 | 0.046 | <0.001 | ||||||||
FGFR1 | 0.005 | |||||||||
GHR | 0.012 | |||||||||
PCOLCE | 0.027 | 0.005 | ||||||||
S1PR1 | 0.033 | |||||||||
TGFBR2 | 0.002 | 0.004 | ||||||||
VEGFC | 0.012 | <0.000 | ||||||||
Differences (FVB-MSCs) | 2 | 2 | 8 | 4 | 12 |
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Churchman, S.M.; Jones, E.A.; Roshdy, T.; Cox, G.; Boxall, S.A.; McGonagle, D.; Giannoudis, P.V. Transient Existence of Circulating Mesenchymal Stem Cells in the Deep Veins in Humans Following Long Bone Intramedullary Reaming. J. Clin. Med. 2020, 9, 968. https://doi.org/10.3390/jcm9040968
Churchman SM, Jones EA, Roshdy T, Cox G, Boxall SA, McGonagle D, Giannoudis PV. Transient Existence of Circulating Mesenchymal Stem Cells in the Deep Veins in Humans Following Long Bone Intramedullary Reaming. Journal of Clinical Medicine. 2020; 9(4):968. https://doi.org/10.3390/jcm9040968
Chicago/Turabian StyleChurchman, Sarah M, Elena A Jones, Tarek Roshdy, George Cox, Sally A Boxall, Dennis McGonagle, and Peter V Giannoudis. 2020. "Transient Existence of Circulating Mesenchymal Stem Cells in the Deep Veins in Humans Following Long Bone Intramedullary Reaming" Journal of Clinical Medicine 9, no. 4: 968. https://doi.org/10.3390/jcm9040968