Combined DNase and Proteinase Treatment Interferes with Composition and Structural Integrity of Multispecies Oral Biofilms
Abstract
:1. Introduction
2. Materials and Methods
2.1. In Vitro Biofilm Experiments
2.2. Staining of Biofilms
2.3. Confocal Laser Scanning Microscopy (CLSM)
2.4. Statistical Analysis
3. Results
3.1. DNase I Significantly Suppressed the Growth of A. oris, F. nucleatum, S. mutans, S. oralis and C. albicans
3.2. Counts of S. mutans, S. oralis and C. albicans Increased after Treatment with Proteinase K
3.3. Combined Treatment with DNase I and Proteinase K Induced a Significant Increase in Total Bacterial, S. mutans, and S. oralis Counts
3.4. Combined Treatment with DNase I and Proteinase K Affected the Structural Integrity and Spatial Distribution of Biofilms
3.5. Fluorescence In Situ Hybridization (FISH)/CLSM Images Highlighted the Dominance of Streptococci after Combined Treatment with DNase I and Proteinase K
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Karygianni, L.; Attin, T.; Thurnheer, T. Combined DNase and Proteinase Treatment Interferes with Composition and Structural Integrity of Multispecies Oral Biofilms. J. Clin. Med. 2020, 9, 983. https://doi.org/10.3390/jcm9040983
Karygianni L, Attin T, Thurnheer T. Combined DNase and Proteinase Treatment Interferes with Composition and Structural Integrity of Multispecies Oral Biofilms. Journal of Clinical Medicine. 2020; 9(4):983. https://doi.org/10.3390/jcm9040983
Chicago/Turabian StyleKarygianni, Lamprini, Thomas Attin, and Thomas Thurnheer. 2020. "Combined DNase and Proteinase Treatment Interferes with Composition and Structural Integrity of Multispecies Oral Biofilms" Journal of Clinical Medicine 9, no. 4: 983. https://doi.org/10.3390/jcm9040983