DNA Fragmentation in Viable and Non-Viable Spermatozoa Discriminates Fertile and Subfertile Subjects with Similar Accuracy
Abstract
:1. Introduction
2. Material and Methods
2.1. Subjects and Sample Collection and Preparation
2.2. LiveTUNEL
2.3. Flow Cytometric Analyses
2.4. Statistical Analysis
3. Results
3.1. LiveTUNEL
3.2. sDF in Fertile Men and in Patients
3.3. SDF as a Predictor of Fertile versus Subfertile Status
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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All Subjects | Patients | Fertile Men | p# | |
---|---|---|---|---|
Number of Subjects | 162 | 91 | 71 | |
Age (y) | 38.00 (34.00–42.00) | 38.00 (35.00–42.00) | 36.00 (34.00–42.00) | 0.121 |
Sperm Number (millions/ejaculate) | 175.75 (106.35–271.80) | 153.00 (67.84–266.60) | 185.49 (114.40–285.00) | 0.133 |
Concentration (106/mL) | 52.50 (33.00–102.20) | 46.00 (22.00–88.00) | 62.00 (38.60–121.50) | 0.067 |
Total Motility (%) | 60.00 (51.75–70.00) | 57.00 (47.00–64.00) | 65.00 (57.00–74.00) | <0.001 |
Progressive Motility (%) | 50.00 (40.00-60.00) | 45.00 (33.00–54.00) | 54.00 (47.00–64.00) | <0.001 |
Morphology (%) | 5.00 (3.00–9.00) | 5.00 (2.00–8.00) | 7.00 (4.00–9.00) | <0.001 |
Abstinence (d) | 4.00 (3.00–4.25) | 4.00 (3.00–5.00) | 3.00 (3.00–4.00) | 0.012 |
Semen Volume (ml) | 3.10 (2.48–4.60) | 3.20 (2.50–4.60) | 3.10 (2.30–4.50) | 0.125 |
Variable | Total sDF | Viable sDF | Non−Viable sDF |
---|---|---|---|
r | r | r | |
p | p | p | |
Age, y | 0.189 * | 0.086 | 0.219 * |
0.016 | 0.278 | 0.005 | |
Sperm Number (×106/ejaculate) | −0.061 | −0.088 | 0.022 |
0.444 | 0.264 | 0.785 | |
Concentration (106/mL) | −0.03 | −0.034 | 0.064 |
0.705 | 0.67 | 0.418 | |
Total Motility (%) | −0.277 * | −0.12 | −0.202 * |
0 | 0.128 | 0.01 | |
Progressive Motility (%) | −0.282 * | −0.136 | −0.183 * |
0 | 0.084 | 0.02 | |
Morphology (%) | −0.256 * | −0.101 | −0.159 * |
0.001 | 0.202 | 0.044 | |
Abstinence, d | 0.203 * | 0.074 | 0.231 * |
0.01 | 0.352 | 0.003 |
AUC (95% CI) | SE | p-Value | |
---|---|---|---|
Total sDF | 0.696 (0.615–0.776) | 0.041 | <0.001 |
Viable sDF | 0.718 (0.640–0.797) | 0.040 | <0.001 |
Non-viable sDF | 0.760 (0.685–0.835 | 0.038 | <0.001 |
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Muratori, M.; Pellegrino, G.; Mangone, G.; Azzari, C.; Lotti, F.; Tarozzi, N.; Boni, L.; Borini, A.; Maggi, M.; Baldi, E. DNA Fragmentation in Viable and Non-Viable Spermatozoa Discriminates Fertile and Subfertile Subjects with Similar Accuracy. J. Clin. Med. 2020, 9, 1341. https://doi.org/10.3390/jcm9051341
Muratori M, Pellegrino G, Mangone G, Azzari C, Lotti F, Tarozzi N, Boni L, Borini A, Maggi M, Baldi E. DNA Fragmentation in Viable and Non-Viable Spermatozoa Discriminates Fertile and Subfertile Subjects with Similar Accuracy. Journal of Clinical Medicine. 2020; 9(5):1341. https://doi.org/10.3390/jcm9051341
Chicago/Turabian StyleMuratori, Monica, Giulia Pellegrino, Giusi Mangone, Chiara Azzari, Francesco Lotti, Nicoletta Tarozzi, Luca Boni, Andrea Borini, Mario Maggi, and Elisabetta Baldi. 2020. "DNA Fragmentation in Viable and Non-Viable Spermatozoa Discriminates Fertile and Subfertile Subjects with Similar Accuracy" Journal of Clinical Medicine 9, no. 5: 1341. https://doi.org/10.3390/jcm9051341