Neutrophil Extracellular Traps Generation Relates with Early Stage and Vascular Complications in Systemic Sclerosis
Abstract
:1. Introduction
2. Patients and Methods
2.1. Study Population
2.2. Data Collection
2.3. Antinuclear Antibody Detection
2.4. Polymorphonuclear Neutrophils (PMN) Isolation and Stimulation
2.5. Visualization and Quantification of Neutrophil Extracellular Traps (NETs)
2.6. Statistical Analysis
3. Results
3.1. Subject Characteristics
3.2. PMN from Systemic Sclerosis (SSc) Patients Generate More NETs Than Those from Controls
3.3. Involvement of Both PMN Activation Status and Serum Factors in the Level of NET Production Is Not Dependent on AAb Specificity in SSc
3.4. NETosis Is Higher in SSc Patients with Vascular Complications But Is Not Dependent on SSc Cutaneous Phenotype
3.5. NETosis Is an Early Process Occurring in SSc That Lasts in SSc Patients with Vascular Complications
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Appendix A
References
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Main Features | SSc Patients | Controls | ||
---|---|---|---|---|
Whole SSc Population | Diffuse SSc | Limited SSc | ||
Number of individuals | 34 | 8 | 26 | 26 |
Age (mean, years) | 59.5 (±10.9) | 55.3 (±11.7) | 60.8 (±10.6) | 60.5 (±11.2) |
Women (%) | 29 (85.3) | 5 (62.5) | 24 (92.3) | 19 (73.1) |
Disease’s duration (years) | 8.6 (±6) | 5 (± 3.5) | 9.8 (± 6.7) | - |
Modified Rodnan score | 11.8 (±8.7) | 25.4 (± 6.2) | 7.6 (± 6) | - |
Internal organ involvement | - | |||
Interstitial lung disease | 9 (26.5) | 3 (37.5) | 6 (23.1) | - |
Ischemic digital ulcers | 8 (23.5) | 3 (37.5) | 5 (19.2) | - |
Pulmonary arterial hypertension | 3 (8.8) | 0 (0) | 3 (11.5) | - |
Scleroderma renal crisis | 2 (5.9) | 2 (25) | 0 (0) | - |
Auto-antibody | - | |||
Anti-topoisomerase I | 8 (23.5) | 3 (37.5) | 5 (19.2) | - |
Anti-centromere | 17 (50) | 0 (0) | 17 (65.4) | - |
Anti-RNA polymerase III | 2 (5.9) | 2 (25) | 0 (0) | - |
Anti-U1RNP | 1 (2.9) | 0 (0) | 1 (3.9) | - |
Anti-U3RNP | 2 (5.9) | 2 (25) | 0 (0) | - |
Anti-NOR90 | 1 (2.9) | 1 (12.5) | 0 (0) | - |
Undetermined | 3 (8.8) | 0 (0) | 3 (11.5) | - |
Treatment at the time of blood collection | - | |||
Immunosuppressive drug (including corticosteroids) | 2 (5.9) | 2 (25) | 0 (0) | - |
Vasodilator drug | 8 (23.5) | 1 (12.5) | 7 (26.9) | - |
Characteristics | SSc Patients without Complication | SSc Patients with Fibrotic Complications | SSc Patients with Vascular Complications | SSc Patients with both Fibrotic and Vascular Complications |
---|---|---|---|---|
Number of patients | 12 | 9 | 8 | 5 |
Mean of disease duration, year (SD) | 8 (±4) | 13.6 (±15.6) | 7.3 (±7.6) | 3.8 (4.2) |
Diffuse cutaneous involvement (%) | - | 3 (33.3) | - | 5 (100) |
Interstitial lung disease (%) | - | 8 (88.9) | - | 1 (20) |
Ischemic digital ulcer(s) (%) | - | - | 5 (62.5) | 3 (60) |
Pulmonary arterial hypertension (%) | - | - | 3 (37.5) | 0 (0) |
Scleroderma renal crisis (%) | - | - | 0 (0) | 2 (40) |
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Didier, K.; Giusti, D.; Le Jan, S.; Terryn, C.; Muller, C.; Pham, B.N.; Le Naour, R.; Antonicelli, F.D.; Servettaz, A. Neutrophil Extracellular Traps Generation Relates with Early Stage and Vascular Complications in Systemic Sclerosis. J. Clin. Med. 2020, 9, 2136. https://doi.org/10.3390/jcm9072136
Didier K, Giusti D, Le Jan S, Terryn C, Muller C, Pham BN, Le Naour R, Antonicelli FD, Servettaz A. Neutrophil Extracellular Traps Generation Relates with Early Stage and Vascular Complications in Systemic Sclerosis. Journal of Clinical Medicine. 2020; 9(7):2136. https://doi.org/10.3390/jcm9072136
Chicago/Turabian StyleDidier, Kevin, Delphine Giusti, Sebastien Le Jan, Christine Terryn, Celine Muller, Bach Nga Pham, Richard Le Naour, Frank D. Antonicelli, and Amelie Servettaz. 2020. "Neutrophil Extracellular Traps Generation Relates with Early Stage and Vascular Complications in Systemic Sclerosis" Journal of Clinical Medicine 9, no. 7: 2136. https://doi.org/10.3390/jcm9072136