Next Article in Journal
Strategy for Use of Genome-Wide Non-Invasive Prenatal Testing for Rare Autosomal Aneuploidies and Unbalanced Structural Chromosomal Anomalies
Next Article in Special Issue
Root Canal Transportation after Root Canal Preparation with ProTaper Next, WaveOne Gold, and Twisted Files
Previous Article in Journal
Impact of Intensive Glucose Control in Patients with Diabetes Mellitus Undergoing Percutaneous Coronary Intervention: 3-Year Clinical Outcomes
Previous Article in Special Issue
Sodium Hypochlorite Reduces Postoperative Discomfort and Painful Early Failure after Carious Exposure and Direct Pulp Capping—Initial Findings of a Randomized Controlled Trial
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
JCM
Volume 9
Issue 8
10.3390/jcm9082465
jcm-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Effect of Sonic Agitation of a Binary Mixture of Solvents on Filling Remnants Removal as an Alternative to Apical Enlargement—A Micro-CT Study

by
Inês Ferreira
1,2,*,
Pedro S. Babo
3,4,
Ana Cristina Braga
5,
Manuela E. Gomes
3,4 and
Irene Pina-Vaz
2,*
1
Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal
2
CINTESIS, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal
3
3B’s Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimarães, Portugal
4
ICVS/3B’s – PT Government Associate Laboratory, 4805-017 Braga/Guimarães, Portugal
5
Department of Production and Systems, ALGORITMI Center, University of Minho, Campus de Gualtar 4710-057 Braga, Portugal
*
Authors to whom correspondence should be addressed.
J. Clin. Med. 2020, 9(8), 2465; https://doi.org/10.3390/jcm9082465
Submission received: 1 July 2020 / Revised: 24 July 2020 / Accepted: 27 July 2020 / Published: 1 August 2020
(This article belongs to the Special Issue Clinical Research of Endodontic Dentistry)
Browse Figure
Versions Notes

Abstract

:
Background: This work aimed to evaluate the efficacy of sonic agitation of a binary mixture of solvents (methyl ethyl ketone/tetrachloroethylene) on filling remnants removal and compare the effects of solvent agitation with the enlargement to the next instrument size. Methods: Twenty-four mandibular incisors were prepared with ProTaper Next (X1, X2) and obturated with the single-cone technique and AH Plus sealer. The teeth were retreated with ProTaper Universal Retreatment and ProTaper Next and divided into two groups (n = 12) according to the final instrument (X3 or X4). All canals were submitted to a supplementary procedure consisting of a mixture of solvents―methyl ethyl ketone/tetrachloroethylene, agitated with EndoActivator. The volume of filling remnants was assessed through micro-computed tomography in the apical 5 mm. Statistical analysis was performed with a significance level of 5%. Results: The supplementary procedure of agitation of the solvent mixture was beneficial in both groups (p < 0.05). There were no statistically significant differences between canals re-prepared until X4 and canals re-prepared until X3 plus solvent (p > 0.05). Conclusions: An additional step with a two-solvent solution potentiated by EndoActivator showed to be very effective for the removal of gutta-percha and resinous sealer remnants from apical root canals of mandibular incisors, avoiding further enlargement.

1. Introduction

Non-surgical endodontic retreatment is challenging and, although there have been advancements in instrumentation technology, the predictability of filling material removal is still low. Strategies for cleaning and disinfection will essentially depend on either supplementary approaches or enlargement to even larger sizes [1,2,3,4]. Adjunctive procedures show controversial outcomes and still do not enable the thorough cleaning of root canals [5,6,7,8,9,10]. Using a larger preparation size would be expected to result in fewer unprepared areas and, consequently, a higher volume of old filling material removal, optimizing disinfection [7,10,11,12,13]. Nevertheless, a correlation between disinfection and optimal canal size was failed to be proved [14,15].
Concerns about the cytotoxic potential of using solvents and the associated risk of hindering the cleaning process of root canal walls and isthmuses made them fall into disuse [8,16,17,18]. The efficacy of solvents for retreatment purposes has been assessed in micro-computed tomographic studies (micro-CT), but the results are inconclusive [8,19,20,21].
Previous research suggested a non-traditional solvent-methyl ethyl ketone (MEK)―for dissolving the AH Plus sealer [22] and tetrachloroethylene for gutta-percha [23], both potentiated by agitation, as an additional and final step in the removal of obturation material remnants. We hypothesize that the binary mixture of solvents might have a complementary dissolution advantage, aside from the easiness of being a single-step procedure, thus overcoming the limitations of re-preparation in the removal of the materials left behind and preventing weakening of the teeth.
No study has evaluated if any adjunctive procedure would result in a filling removal similar to that of larger instrumentation. Therefore, this work aimed to evaluate through micro-CT analysis the efficacy of sonic agitation of a mixture of solvents (MEK/tetrachloroethylene (1:1)) on filling material remnant removal and compare the effects of the additional solvent agitation with the enlargement to the next instrument size.

2. Experimental Section

2.1. Specimen Selection

After approval by the Ethics Committee of the Faculty of Dental Medicine of the University of Porto (nº3/2018), the teeth were selected from a group of 100 mandibular central single-rooted incisors extracted for reasons unrelated to this study. Mesiodistal and buccolingual radiographs were taken, and teeth with a single canal (Vertucci type 1) were selected. Each tooth was then inspected under an operating microscope, and all teeth with root fractures, internal or external root resorptions, and immature apex were excluded. The teeth were gauged using a stainless-steel K-file (Dentsply Sirona, Ballaigues, Switzerland), and only those where the first instrument to bind at working length was a size 15 were selected. Twenty-four teeth were selected according to these criteria.

2.2. Root Canal Preparation and Filling

Teeth were stabilized during the preparation procedures. A size 10 K-file was used to determine the working length (WL), which was about 1 mm shorter than the full root-canal length. The canals were prepared with ProTaper Next (X1, X2), and repeatedly irrigated using 3% NaOCl (30G needle-Max-I-Probe, Dentsply International, Inc., York, PA, USA) after each file (a total of 5 mL of NaOCl was used). The apical patency was checked with a size 10 K-type file. The final rinse was performed with 2 mL of 3% NaOCl followed by 2 mL of 17% EDTA for 1 min, both sonically agitated for 1 min (three periods of 20 s) with EndoActivator (Dentsply Sirona, Ballaigues, Switzerland, (size 25.04 taper and 10,000 cycles/min; 2 mm short of the WL)) and, finally, 1 mL of distilled water. After drying with paper points, the root canals were filled with ProTaper Next X2 gutta-percha cone (Dentsply Sirona, Ballaigues, Switzerland) and AH Plus Jet (Dentsply DeTrey, Konstanz, Germany), using the single-cone technique. The sealer was placed using a lentulo spiral filler. The roots were radiographed in buccolingual and mesiodistal directions to ensure consistency of the root filling procedure. Teeth in which the root canal filling was judged unsatisfactory were replaced by new samples. The access cavities were sealed with a temporary restorative material (IRM, Dentsply Sirona, Ballaigues, Switzerland), and the samples were stored in 100% humidity at 37 °C for 2 weeks.

2.3. Retreatment and Re-Preparation Procedures

Retreatment was performed with ProTaper Universal Retreatment (D1, D2, and D3) and Hedström files (Dentsply Sirona, Ballaigues, Switzerland). ProTaper files D1, D2, and D3 were used for the removal of the bulk of filling material at the coronal, middle and apical thirds of the root canals, respectively. The files were removed from the canal, cleaned with a sponge, and placed again until the required length was achieved. For the refinement of the residual filling material removal, or whenever necessary to reach the WL, Hedström files of size 30 and 25 were used with brushing or circumferential quarter-turn push-pull filling motions, allowing better control of the cleanliness of root walls. Patency was checked with a size 15 K-type hand file. A total of 15 mL of 3% NaOCl was used.
The filling materials were considered to be completely removed when no remaining filling materials were observed inside the root canal, on the ProTaper Next or Hedström instrument, or floating in the irrigation solution, with the help of a microscope (OPMI Pico, Carl Zeiss, Jena, Germany).
Teeth were randomly allocated into two groups of 12 specimens:
-
Group 1: Re-preparation with ProTaper Next X3 file
-
Group 2: Re-preparation with ProTaper Next X3 and X4 files
Teeth were prepared following the same protocol used in the instrumentation of the canals, using a total of 5 mL of 3% NaOCl, and a final irrigation was performed with 2 mL of 3% NaOCl, followed by 2 mL of 17% EDTA, both activated with EndoActivator for 1 min.

2.4. Supplementary Procedure

All specimens were submitted to a supplementary procedure consisting of using a binary mixture of solvents―MEK/tetrachloroethylene (1:1) (VWR International SAS, Fontenay-sous-Bois, France), sonically agitated. Each canal was rinsed with a total of 1 mL of the solvent mixture agitated sonically for 5 min using the EndoActivator (red tip, 25.04 at a speed of 10,000 cycles/min with “in-and-out” movements; 2 mm short of the WL). The mixture was refreshed after each minute. Lastly, all the canals were irrigated with 5 mL of distilled water to neutralize the action of the mixture of solvents.

2.5. Micro-CT Imaging Analysis

The samples were scanned at three moments: After root canal filling, after conventional retreatment/re-preparation, and after the supplementary procedure (solvent/EndoActivator), using a high-resolution micro-CT (Skyscan 1272, Bruker, Kontich, Belgium). A series of two-dimensional projections of the specimens with a resolution of 10 μm were acquired by irradiation with penetrative X-rays and using a 0.5-mm-thick aluminum filter. This acquisition was made over a rotation range of 360° with a rotation step of 0.45°. Data were reconstructed using the software NRecon (version 1.7.1.0, Bruker, Kontich, Belgium) and analyzed on a CT analyzer (version 1.17.0.0, Bruker, Kontich, Belgium). The region of interest (ROI) was defined as a circle centered in the root canal. For standardization, the apical region was analyzed between 1 and 5 mm above the apex opening. An auto-interpolation of the manually defined ROI was conducted to obtained volumes of interest (VOI) corresponding to the apex regions of 1–3 mm and 3–5 mm, which were the essential basis for the quantitative analyses. The three-dimensional models were generated using the software CT Vox (version 3.3.0 r1412, Bruker, Kontich, Belgium).
All specimens were prepared by a single operator, following standard clinical endodontic practices, and the procedures are schematically illustrated in Figure 1. Each instrument was used in three teeth and then discarded.

2.6. Statistical Analysis

The sample calculation was performed using the G*Power software, version 3.1.9.6 (Franz Faul, Universität Düsseldorf, Düsseldorf, Germany) [24]. The data from a previous study [25] on retreatment using single-rooted teeth were used to determine the effect size for the present study (i.e., 1.80). For unilateral testing, an alpha-type error of 0.05, a beta power of 0.95, and a ratio of N2/N1 = 1 were also stipulated. A family of tests used for the differences in means between two independent groups indicated eight specimens per group as the ideal size. Considering 12 specimens per group, the power increases to 0.996.
The Kolmogorov–Smirnov (KS) test was applied to test for data normality. Based on the KS results, statistical analysis to assess differences between groups was performed using the Student’s t-test or the Mann–Whitney test, depending on whether normality was verified or not, respectively. A paired t-test was used to compare the volume of filling material remnants in the samples prepared by each technique. For all statistical tests, the level of significance was established at 5% (p < 0.05).

3. Results

Table 1 shows the mean and standard deviation values of the filling materials at the different root sections. The t-test confirmed a similar volume of filling materials before retreatment between groups and sections (p = 0.385 for 1–3 mm; p = 0.496 for 3–5 mm; p = 0.417 for 1–5 mm), which allowed the comparison between groups.
A significant reduction in the filling materials after re-preparation was detected in the two groups for both the assessed regions. Group 1 (re-preparation until X3) presented more remnants than Group 2 (re-preparation until X4), both for the sections and the full apical canal, without statistically significant differences (p = 0.200 for 1–3 mm; p = 0.136 for 3–5 mm; p = 0.087 for 1–5 mm).
There were also no significant differences between Group 1 and 2 after using the solvent (p = 0.974 for 1–3 mm; p = 0.622 for 3–5 mm; p = 0.412 for 1–5 mm). However, both groups showed significant differences (p < 0.05) in the amount of remnants from re-preparation (until X3 or X4) to after solvent exposure. Thus, the supplementary procedure with the application of the mixture of solvents was beneficial in both groups. Additionally, no statistically significant differences were found between re-preparation until X4 before solvent (Group 2) and re-preparation until X3 after solvent (Group 1) (p = 0.073 for 1–3 mm; p = 0.959 for 3–5 mm; p = 0.358 for 1–5 mm).

4. Discussion

The present investigation aimed to study the effectiveness of the sonic agitation of a mixture of solvents on the volume of filling material remnants in mandibular incisors, and to assess if it would compare to progressive apical enlargement. Similar to other investigations, the sample was standardized, based on an initial radiographic evaluation and apical gauging, to avoid relevant bias regarding canal anatomy and filling material volume [11,12]. Statistical analysis revealed a similar volume of filling materials before retreatment and between groups and sections (Table 1).
The apical third of the root canal is considered the most critical zone for thorough disinfection due to its anatomical complexities and persistence of accumulated debris and bacterial biofilms [26,27]. Thus, filling materials were mapped in the apical 5 mm using micro-CT after root canal filling, after mechanical re-preparation, and after the adjunctive final solvent protocol [7]. The volume of the residual filling material was calculated for the full apical canal (5 mm from the apex) and the different Sections 1–3 and 3–5 mm from the apex [8].
Patency was re-established in all root canals, but in around 60% of the samples, this was only after solvent exposure. The enlargement from an X3 to an X4 ProTaper Next file did not statistically significantly decrease the volume of filling remnants, both in the full apical canal and in the 1–3 mm and 3–5 mm sections. However, the solvents’ mixture applied (MEK/tetrachloroethylene), agitated by a sonic device (EndoActivator), significantly decreased filling remnants. Root canals were cleaner after solvent exposure than without solvent, both with the X3 and the X4 re-preparation (Figure 1 and Table 1). Additionally, root canals prepared to a larger size (size 40) were as clean as smaller preparations (size 30) after the sonically agitated solvent mixture. Accordingly, it was recently reported that when sodium hypochlorite was ultrasonically activated, smaller preparations resulted as clean as larger preparations [28]. Even though the referenced study evaluated the remaining pulp tissue and debris instead of residual filling materials, the results demonstrate the influence of the irrigation strategy overcoming the insufficiencies of the instrumentation approach, irrespective of the canal configuration (round vs. oval). Further research should focus particularly on the effect of different apical preparation sizes in conjunction with activated irrigation and whether it would result in similar filling removal [12,28].
An increased enlargement of apical re-preparation has been associated with a lower volume of residual filling materials [7,10,12]. This difference has been reported between additional instrumentation with larger tip sizes (size 35–40) compared to size 25. In the present study, the results showed that an increase to the next instrument size (tip size 30 to 40) might not directly correspond to a decrease in residual obturation material volume. Different methodologies, tooth types, instrument sizes, and motions can influence the shaping ability of the instruments [1,4,6,29,30,31,32]. Even though the number of unprepared areas was shown to be inversely proportional to the increase of each preparation size, a consequent thinner remaining dentin can compromise the tooth’s survival [11,28,33]. Additionally, a larger apical size did not result in a complete apical preparation, but it could lead to unnecessary removal of dentin, with the risk of iatrogenic procedures [34,35]. Filling remnants remaining in untouched recesses and isthmus might prevent irrigants and dressings from reaching biofilms, not allowing for complete root canal wall exposure. An additional step with an effective solvent for both filling materials should improve disinfection, by further removing potentially infected filling remnants and avoiding unnecessary enlargement or risk of procedural errors. Besides the improved dissolution efficacy reported, the agitation should promote better solvents’ dispersion, reaching areas usually inaccessible to endodontic instruments, and thus improving root canal cleanliness [6,36,37].
Recently developed finishing files, such as XP-endo Finisher R (FKG Dentaire, La Chaud-de-Fonds, Switzerland), have been highlighted as an effective supplementary approach. However, they are advised to be used following preparations with apical diameters of 30 or more [7,10,38]. New design ultrasonic tips have also shown good performance, but, again, the additional removal of dentine may be a disadvantage [39].
Previous studies found no differences in the volume of obturation materials removed with the use of traditional endodontic solvents, independently of ultrasonic agitation (PUI) [8,20,21]. AH Plus is an epoxy resin-based sealer commonly used due to its adequate properties as an endodontic sealer [40]. Targeting the sealer chemistry proved to be crucial in the achievement of solvent efficacy for retreatment purposes [22,41]. These results may be explained, firstly, by the specificity of the solvents in the binary mixture―MEK and tetrachloroethylene—which were described to be effective for epoxy-resin sealer and gutta-percha, respectively. Their efficacy, enhanced by ultrasonic agitation, was reported to be similar to the traditional chloroform, without its potential cytotoxicity [22,23]. The 1:1 mixture would enable a clinical single-step procedure since MEK proved to act mainly on resinous sealer dissolution, while tetrachloroethylene could have an additional effect on the sealer besides gutta-percha dissolution [23]. We opted to use the EndoActivator for the additional cleaning with agitation due to its clinical safety and easiness of use [42]. Sonic and ultrasonic devices have been reported to be equivalent in the removal of filling materials when activating sodium hypochlorite [6]. To our knowledge, there are no reports about the efficacy of sonic agitation of solvents on filling materials removal.
Even though not all the samples were completely free from remnants, due to the time-dependent dissolution effect [22,23], it can be expected that the longer the period of agitation, the better the cleanliness achieved in complex root canal anatomies. Due to the low cytotoxicity profile of the suggested solvents in the mixture, as compared to the traditional chloroform, damage to the dentine or other relevant cytotoxic hazards are not expected, even with longer periods [43,44,45]. Nevertheless, all procedures must follow the gold standard quality guidelines for endodontic treatment [46]. Even though there seems to be a consensus on the use of solvents in the early stages of instrumentation, due to reaching the working length in a shorter time, studies confirming their advantages are still scarce [2].
One of the drawbacks of the present ex-vivo investigation was the lack of matched-pair design by micro-CT [47]. However, recent investigations using pair-matched root canals seem to corroborate the present results, highlighting the role of irrigant agitation in overcoming the insufficiencies of mechanical instrumentation [28]. Further studies that control these potentially confounding effects and assess other tooth anatomies should confirm the present findings.
Overall, our findings suggest that a non-traditional binary mixture of solvents―MEK/tetrachloroethylene (1:1)—potentiated by sonic agitation, as herein proposed, holds the potential to become an additional and final step in retreatment procedures, complementing mechanical cleaning in the removal of the obturation material. It could be used in a single solution with a dual objective of dissolving gutta-percha and a resinous sealer and is presented as an alternative to chloroform without its potential hazards. The strategy proposed might enable the optimization of retreatment procedures to obtain cleaner canals without further enlargement.

Author Contributions

All of the authors contributed to the visualization, writing, review, and editing of the study. Conceptualization, I.F. and I.P.-V.; methodology, I.F., P.S.B.; validation, I.F. and I.P.-V.; formal analysis, I.F., P.S.B., A.C.B.; investigation, I.F., P.S.B.; resources, M.E.G., I.P.-V.; data curation, I.F., P.S.B., A.C.B.; supervision, M.E.G., I.P.-V.; funding acquisition, I.F. and I.P.-V. All authors have read and agreed to the published version of the manuscript.

Funding

This article was supported by National Funds through FCT-Fundação para a Ciência e a Tecnologia, I.P., within CINTESIS, R&D Unit (reference UIDB/4255/2020).

Acknowledgments

The authors thank Dentsply Sirona for the materials used in this study.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Romeiro, K.; de Almeida, A.; Cassimiro, M.; Gominho, L.; Dantas, E.; Chagas, N.; Velozo, C.; Freire, L.; Albuquerque, D. Reciproc and Reciproc Blue in the removal of bioceramic and resin-based sealers in retreatment procedures. Clin. Oral Investig. 2020, 24, 405–416. [Google Scholar] [CrossRef] [PubMed]
  2. Rossi-Fedele, G.; Ahmed, H.M. Assessment of Root Canal Filling Removal Effectiveness Using Micro-computed Tomography: A Systematic Review. J. Endod. 2017, 43, 520–526. [Google Scholar] [CrossRef] [PubMed]
  3. Siqueira, J.F., Jr.; Rocas, I.N. Optimising single-visit disinfection with supplementary approaches: A quest for predictability. Aust. Endod. J. 2011, 37, 92–98. [Google Scholar] [CrossRef] [PubMed]
  4. Alakabani, T.F.; Faus-Llácer, V.; Faus-Matoses, I.; Ruiz-Sánchez, C.; Zubizarreta-Macho, Á.; Sauro, S.; Faus-Matoses, V. The Efficacy of Rotary, Reciprocating, and Combined Non-Surgical Endodontic Retreatment Techniques in Removing a Carrier-Based Root Canal Filling Material from Straight Root Canal Systems: A Micro-Computed Tomography Analysis. J. Clin. Med. 2020, 9, 1989. [Google Scholar] [CrossRef] [PubMed]
  5. Keles, A.; Alcin, H.; Kamalak, A.; Versiani, M.A. Oval-shaped canal retreatment with self-adjusting file: A micro-computed tomography study. Clin. Oral Investig. 2014, 18, 1147–1153. [Google Scholar] [CrossRef]
  6. Martins, M.P.; Duarte, M.A.H.; Cavenago, B.C.; Kato, A.S.; da Silveira Bueno, C.E. Effectiveness of the ProTaper Next and Reciproc Systems in Removing Root Canal Filling Material with Sonic or Ultrasonic Irrigation: A Micro-computed Tomographic Study. J. Endod. 2017, 43, 467–471. [Google Scholar] [CrossRef]
  7. Alves, F.R.; Marceliano-Alves, M.F.; Sousa, J.C.; Silveira, S.B.; Provenzano, J.C.; Siqueira, J.F., Jr. Removal of Root Canal Fillings in Curved Canals Using Either Reciprocating Single- or Rotary Multi-instrument Systems and a Supplementary Step with the XP-Endo Finisher. J. Endod. 2016, 42, 1114–1119. [Google Scholar] [CrossRef]
  8. Barreto, M.S.; Rosa, R.A.; Santini, M.F.; Cavenago, B.C.; Duarte, M.A.; Bier, C.A.; So, M.V. Efficacy of ultrasonic activation of NaOCl and orange oil in removing filling material from mesial canals of mandibular molars with and without isthmus. J. Appl. Oral Sci. 2016, 24, 37–44. [Google Scholar] [CrossRef] [Green Version]
  9. Silveira, S.B.; Alves, F.R.F.; Marceliano-Alves, M.F.; Sousa, J.C.N.; Vieira, V.T.L.; Siqueira, J.F., Jr.; Lopes, H.P.; Provenzano, J.C. Removal of Root Canal Fillings in Curved Canals Using Either Mani GPR or HyFlex NT Followed by Passive Ultrasonic Irrigation. J. Endod. 2018, 44, 299–303. [Google Scholar] [CrossRef]
  10. Borges, M.M.B.; Duque, J.A.; Zancan, R.F.; Vivan, R.R.; Bernardes, R.A.; Duarte, M.A.H. Efficacy of reciprocating systems for removing root filling material plus complementary cleaning methods in flattened canals: Microtomography and scanning electron microscopy study. Microsc. Res. Tech. 2019, 82, 1057–1064. [Google Scholar] [CrossRef]
  11. Perez, A.R.; Alves, F.R.F.; Marceliano-Alves, M.F.; Provenzano, J.C.; Goncalves, L.S.; Neves, A.A.; Siqueira, J.F., Jr. Effects of increased apical enlargement on the amount of unprepared areas and coronal dentine removal: A micro-computed tomography study. Int. Endod. J. 2018, 51, 684–690. [Google Scholar] [CrossRef] [PubMed]
  12. Bago, I.; Plotino, G.; Katić, M.; Ročan, M.; Batinić, M.; Anić, I. Evaluation of filling material remnants after basic preparation, apical enlargement and final irrigation in retreatment of severely curved root canals in extracted teeth. Int. Endod. J. 2020, 53, 962–973. [Google Scholar] [CrossRef] [PubMed]
  13. Aminoshariae, A.; Kulild, J. Master apical file size—Smaller or larger: A systematic review of microbial reduction. Int. Endod. J. 2015, 48, 1007–1022. [Google Scholar] [CrossRef] [PubMed]
  14. Vieira, G.C.S.; Pérez, A.R.; Alves, F.R.F.; Provenzano, J.C.; Mdala, I.; Siqueira, J.F., Jr.; Rôças, I.N. Impact of Contracted Endodontic Cavities on Root Canal Disinfection and Shaping. J. Endod. 2020, 46, 655–661. [Google Scholar] [CrossRef] [PubMed]
  15. Siqueira, J.F., Jr.; Alves, F.R.; Versiani, M.A.; Rôças, I.N.; Almeida, B.M.; Neves, M.A.; Sousa-Neto, M.D. Correlative bacteriologic and micro-computed tomographic analysis of mandibular molar mesial canals prepared by self-adjusting file, reciproc, and twisted file systems. J. Endod. 2013, 39, 1044–1050. [Google Scholar] [CrossRef] [PubMed]
  16. Vajrabhaya, L.O.; Suwannawong, S.K.; Kamolroongwarakul, R.; Pewklieng, L. Cytotoxicity evaluation of gutta-percha solvents: Chloroform and GP-Solvent (limonene). Oral Surg. Oral Med. Oral Pathol. 2004, 98, 756–759. [Google Scholar] [CrossRef]
  17. Kfir, A.; Tsesis, I.; Yakirevich, E.; Matalon, S.; Abramovitz, I. The efficacy of five techniques for removing root filling material: Microscopic versus radiographic evaluation. Int. Endod. J. 2012, 45, 35–41. [Google Scholar] [CrossRef]
  18. Horvath, S.D.; Altenburger, M.J.; Naumann, M.; Wolkewitz, M.; Schirrmeister, J.F. Cleanliness of dentinal tubules following gutta-percha removal with and without solvents: A scanning electron microscopic study. Int. Endod. J. 2009, 42, 1032–1038. [Google Scholar] [CrossRef]
  19. Cavenago, B.C.; Ordinola-Zapata, R.; Duarte, M.A.; del Carpio-Perochena, A.E.; Villas-Boas, M.H.; Marciano, M.A.; Bramante, C.M.; Moraes, I.G. Efficacy of xylene and passive ultrasonic irrigation on remaining root filling material during retreatment of anatomically complex teeth. Int. Endod. J. 2014, 47, 1078–1083. [Google Scholar] [CrossRef]
  20. Campello, A.F.; Almeida, B.M.; Franzoni, M.A.; Alves, F.R.F.; Marceliano-Alves, M.F.; Rocas, I.N.; Siqueira, J.F., Jr.; Provenzano, J.C. Influence of solvent and a supplementary step with a finishing instrument on filling material removal from canals connected by an isthmus. Int. Endod. J. 2019, 52, 716–724. [Google Scholar] [CrossRef]
  21. Fruchi Lde, C.; Ordinola-Zapata, R.; Cavenago, B.C.; Hungaro Duarte, M.A.; Bueno, C.E.; De Martin, A.S. Efficacy of reciprocating instruments for removing filling material in curved canals obturated with a single-cone technique: A micro-computed tomographic analysis. J. Endod. 2014, 40, 1000–1004. [Google Scholar] [CrossRef] [PubMed]
  22. Ferreira, I.; Soares, S.; Sousa, J.; Barros, J.; Braga, A.C.; Lopes, M.A.; Pina-Vaz, I. New Insight into the Dissolution of Epoxy Resin-based Sealers. J. Endod. 2017, 43, 1505–1510. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  23. Ferreira, I.; Braga, A.C.; Lopes, M.A.; Pina-Vaz, I. Improvement of the efficacy of endodontic solvents by ultrasonic agitation. Saudi Dent. J. 2019. [Google Scholar] [CrossRef]
  24. Faul, F.; Erdfelder, E.; Lang, A.-G.; Buchner, A. G*Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav. Res. Methods 2007, 39, 175–191. [Google Scholar] [CrossRef] [PubMed]
  25. Özyürek, T.; Demiryürek, E. Efficacy of Different Nickel-Titanium Instruments in Removing Gutta-percha during Root Canal Retreatment. J. Endod. 2016, 42, 646–649. [Google Scholar] [CrossRef]
  26. Zhao, Y.; Fan, W.; Xu, T.; Tay, F.R.; Gutmann, J.L.; Fan, B. Evaluation of several instrumentation techniques and irrigation methods on the percentage of untouched canal wall and accumulated dentine debris in C-shaped canals. Int. Endod. J. 2019, 52, 1354–1365. [Google Scholar] [CrossRef]
  27. Siqueira, J.F., Jr.; Pérez, A.R.; Marceliano-Alves, M.F.; Provenzano, J.C.; Silva, S.G.; Pires, F.R.; Vieira, G.C.S.; Rôças, I.N.; Alves, F.R.F. What happens to unprepared root canal walls: A correlative analysis using micro-computed tomography and histology/scanning electron microscopy. Int. Endod. J. 2018, 51, 501–508. [Google Scholar] [CrossRef]
  28. Lee, O.Y.S.; Khan, K.; Li, K.Y.; Shetty, H.; Abiad, R.S.; Cheung, G.S.P.; Neelakantan, P. Influence of apical preparation size and irrigation technique on root canal debridement: A histological analysis of round and oval root canals. Int. Endod. J. 2019, 52, 1366–1376. [Google Scholar] [CrossRef]
  29. Nevares, G.; de Albuquerque, D.S.; Freire, L.G.; Romeiro, K.; Fogel, H.M.; Dos Santos, M.; Cunha, R.S. Efficacy of ProTaper NEXT Compared with Reciproc in Removing Obturation Material from Severely Curved Root Canals: A Micro-Computed Tomography Study. J. Endod. 2016, 42, 803–808. [Google Scholar] [CrossRef]
  30. Rodrigues, C.T.; Duarte, M.A.H.; Guimarães, B.M.; Vivan, R.R.; Bernardineli, N. Comparison of two methods of irrigant agitation in the removal of residual filling material in retreatment. Braz. Oral Res. 2017, 31, e113. [Google Scholar] [CrossRef] [Green Version]
  31. Bernardes, R.A.; Duarte, M.A.H.; Vivan, R.R.; Alcalde, M.P.; Vasconcelos, B.C.; Bramante, C.M. Comparison of three retreatment techniques with ultrasonic activation in flattened canals using micro-computed tomography and scanning electron microscopy. Int. Endod. J. 2016, 49, 890–897. [Google Scholar] [CrossRef] [PubMed]
  32. Rödig, T.; Reicherts, P.; Konietschke, F.; Dullin, C.; Hahn, W.; Hülsmann, M. Efficacy of reciprocating and rotary NiTi instruments for retreatment of curved root canals assessed by micro-CT. Int. Endod. J. 2014, 47, 942–948. [Google Scholar] [CrossRef] [PubMed]
  33. Kishen, A. Mechanisms and risk factors for fracture predilection in endodontically treated teeth. Endod. Top. 2006, 13, 57–83. [Google Scholar] [CrossRef]
  34. Elayouti, A.; Dima, E.; Judenhofer, M.S.; Lost, C.; Pichler, B.J. Increased apical enlargement contributes to excessive dentin removal in curved root canals: A stepwise microcomputed tomography study. J. Endod. 2011, 37, 1580–1584. [Google Scholar] [CrossRef]
  35. Hulsmann, M.; Drebenstedt, S.; Holscher, C. Shaping and filling root canals during root canal re-treatment. Endod. Top. 2008, 19, 74–124. [Google Scholar] [CrossRef]
  36. Pacheco-Yanes, J.; Provenzano, J.C.; Marceliano-Alves, M.F.; Gazzaneo, I.; Perez, A.R.; Goncalves, L.S.; Siqueira, J.F., Jr. Distribution of sodium hypochlorite throughout the mesial root canal system of mandibular molars after adjunctive irrigant activation procedures: A micro-computed tomographic study. Clin. Oral Investig. 2020, 24, 907–914. [Google Scholar] [CrossRef]
  37. van der Sluis, L.W.; Versluis, M.; Wu, M.K.; Wesselink, P.R. Passive ultrasonic irrigation of the root canal: A review of the literature. Int. Endod. J. 2007, 40, 415–426. [Google Scholar] [CrossRef]
  38. Silva, E.; Belladonna, F.G.; Zuolo, A.S.; Rodrigues, E.; Ehrhardt, I.C.; Souza, E.M.; De-Deus, G. Effectiveness of XP-endo Finisher and XP-endo Finisher R in removing root filling remnants: A micro-CT study. Int. Endod. J. 2018, 51, 86–91. [Google Scholar] [CrossRef]
  39. De-Deus, G.; Simões-Carvalho, M.; Belladonna, F.G.; Cavalcante, D.M.; Portugal, L.S.; Prado, C.G.; Souza, E.M.; Lopes, R.T.; Silva, E.J.N.L. Arrowhead design ultrasonic tip as a supplementary tool for canal debridement. Int. Endod. J. 2020, 53, 410–420. [Google Scholar] [CrossRef]
  40. Silva, E.J.; Perez, R.; Valentim, R.M.; Belladonna, F.G.; De-Deus, G.A.; Lima, I.C.; Neves, A.A. Dissolution, dislocation and dimensional changes of endodontic sealers after a solubility challenge: A micro-CT approach. Int. Endod. J. 2017, 50, 407–414. [Google Scholar] [CrossRef]
  41. Garrib, M.; Camilleri, J. Retreatment efficacy of hydraulic calcium silicate sealers used in single cone obturation. J. Dent. 2020, 98, 103370. [Google Scholar] [CrossRef] [PubMed]
  42. Ruddle, C.J. Hydrodynamic disinfection: Tsunami endodontics. Dent. Today 2007, 26, 114–117. [Google Scholar] [PubMed]
  43. Verma, P.; Tordik, P.; Nosrat, A. Hazards of Improper Dispensary: Literature Review and Report of an Accidental Chloroform Injection. J. Endod. 2018, 44, 1042–1047. [Google Scholar] [CrossRef] [PubMed]
  44. EPA. United States Environmental Protection Agency. Tetrachloroethylene (Perchloroethylene). Available online: https://www.epa.gov/sites/production/files/2016-09/documents/tetrachloroethylene.pdf (accessed on 7 March 2020).
  45. EPA. Methyl Ethyl Ketone (2-Butanone). Available online: https://www.epa.gov/sites/production/files/2016-09/documents/methyl-ethyl-ketone.pdf (accessed on 7 March 2020).
  46. ESE. Quality guidelines for endodontic treatment: Consensus report of the European Society of Endodontology. Int. Endod. J. 2006, 39, 921–930. [Google Scholar] [CrossRef]
  47. De-Deus, G.; Simões-Carvalho, M.; Belladonna, F.G.; Versiani, M.A.; Silva, E.; Cavalcante, D.M.; Souza, E.M.; Johnsen, G.F.; Haugen, H.J.; Paciornik, S. Creation of well-balanced experimental groups for comparative endodontic laboratory studies: A new proposal based on micro-CT and in silico methods. Int. Endod. J. 2020, 53, 974–985. [Google Scholar] [CrossRef] [PubMed]
Jcm 09 02465 g001 550
Figure 1. Micro-computed tomographic (Micro-CT) images of representative specimens subjected to the different procedures (obturation with X2 gutta-percha cone and AH Plus sealer (G1 and G2); re-preparation until X3 (G1) and X4 (G2); after solvent/EndoActivator (G1 and G2). Green: Gutta-percha; red: Sealer.
Figure 1. Micro-computed tomographic (Micro-CT) images of representative specimens subjected to the different procedures (obturation with X2 gutta-percha cone and AH Plus sealer (G1 and G2); re-preparation until X3 (G1) and X4 (G2); after solvent/EndoActivator (G1 and G2). Green: Gutta-percha; red: Sealer.
Jcm 09 02465 g001
Table
Table 1. Volume (mm3) of filling material in different sections (1–3 mm and 3–5 mm) of the apical portion (1–5 mm). (G1: Canals re-prepared until X3 file; G2: Canals re-prepared until X4 file).
Table 1. Volume (mm3) of filling material in different sections (1–3 mm and 3–5 mm) of the apical portion (1–5 mm). (G1: Canals re-prepared until X3 file; G2: Canals re-prepared until X4 file).
ObturationRe-PreparationAfter Solvent/EndoActivator
G1G2TotalG1G2TotalG1G2Total
1–3 mm0.260
(± 0.041)		0.246
(± 0.035)		0.252
(± 0.038)		0.051
(± 0.035)		0.036
(± 0.022)		0.043
(± 0.029) a		0.018
(± 0.024)		0.013
(± 0.014)		0.015
(± 0.019) a
3–5 mm0.483
(± 0.077)		0.453
(± 0.124)		0.468
(± 0.103)		0.057
(± 0.050)		0.030
(± 0.031)		0.043
(± 0.043) b		0.030
(± 0.033)		0.019
(± 0.022)		0.024
(± 0.028) b
1–5 mm0.743
(± 0.104)		0.699
(± 0.146)		0.720
(± 0.127)		0.108
(± 0.065)		0.066
(± 0.048)		0.086
(± 0.059) c		0.047
(± 0.047)		0.031
(± 0.029)		0.039
(± 0.039) c
a,b,c―t-test revealed significant differences p < 0.05.

Share and Cite

MDPI and ACS Style

Ferreira, I.; Babo, P.S.; Braga, A.C.; Gomes, M.E.; Pina-Vaz, I. Effect of Sonic Agitation of a Binary Mixture of Solvents on Filling Remnants Removal as an Alternative to Apical Enlargement—A Micro-CT Study. J. Clin. Med. 2020, 9, 2465. https://doi.org/10.3390/jcm9082465

AMA Style

Ferreira I, Babo PS, Braga AC, Gomes ME, Pina-Vaz I. Effect of Sonic Agitation of a Binary Mixture of Solvents on Filling Remnants Removal as an Alternative to Apical Enlargement—A Micro-CT Study. Journal of Clinical Medicine. 2020; 9(8):2465. https://doi.org/10.3390/jcm9082465

Chicago/Turabian Style

Ferreira, Inês, Pedro S. Babo, Ana Cristina Braga, Manuela E. Gomes, and Irene Pina-Vaz. 2020. "Effect of Sonic Agitation of a Binary Mixture of Solvents on Filling Remnants Removal as an Alternative to Apical Enlargement—A Micro-CT Study" Journal of Clinical Medicine 9, no. 8: 2465. https://doi.org/10.3390/jcm9082465

APA Style

Ferreira, I., Babo, P. S., Braga, A. C., Gomes, M. E., & Pina-Vaz, I. (2020). Effect of Sonic Agitation of a Binary Mixture of Solvents on Filling Remnants Removal as an Alternative to Apical Enlargement—A Micro-CT Study. Journal of Clinical Medicine, 9(8), 2465. https://doi.org/10.3390/jcm9082465

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop