Variation in Protein and Isoflavone Contents of Collected Domestic and Foreign Soybean (Glycine max (L.) Merrill) Germplasms in Korea
Round 1
Reviewer 1 Report
Introduction should contain more recently references.
Line 44 - 47: These sentences are confused, are contradictory, please reformulated.
Line 89: Uniformized the units according to the journal' instructions.
Line 119: HPLC analysis conditions must be described on tis section, not in table as supplementary material.
Line 134: Mistake on 99,33%, must be 99.33%. The number of decimal should be the same trough the manuscript.
Protein content are in % and the isoflavone content µg g-1. Please uniformized
Line 140: Replace p-value <0.0001 by p <0.001, and in all manuscript.
Fig. 1, Fig. 2, Table 5, Table 6, Table 7: Include a space before the parentheses
There are two Figure 2.
Footnote of Table 3, replace 100 seed weight: 24g< by 100 seed weight: <24g
Line 279: mistake in "difference".
Author Response
Line 44 - 47: These sentences are confused, are contradictory, please reformulated.
Corrected it
Line 89: Uniformized the units according to the journal' instructions.
Corrected it
Line 119: HPLC analysis conditions must be described on its section, not in table as supplementary material.
Added HPLC analysis
Line 134: Mistake on 99,33%, must be 99.33%. The number of decimal should be the same trough the manuscript.
Corrected it
Protein content are in % and the isoflavone content µg g-1. Please uniformized
Line 140: Replace p-value <0.0001 by p <0.001, and in all manuscript.
Corrected it
Fig. 1, Fig. 2, Table 5, Table 6, Table 7: Include a space before the parentheses
There are two Figure 2.
Corrected it
Footnote of Table 3, replace 100 seed weight: 24g< by 100 seed weight: <24g
Corrected it
Line 279: mistake in "difference".
Corrected it
Reviewer 2 Report
- In row 91 to 92: The harvested soybean germplasms were stored in a -20°C freezer and used for protein and isoflavone analysis. What does germplasms mean here? Seeds, leaves, stems, or whole plants? Need clarify the term germplasm here.
- In row 100: analysed by the micro-Kjeldahl method. Here needs a reference
- In row 125: All data were subjected to analysis of variance (ANOVA) via the SAS software package. There was NO any report about the ANOVA analysis. The manuscript will be better if a ANOVA table reported in the main text to tell the interactions seed coar color, region, and size.
- In row 228 to 231: The correlation coefficients for protein compared to daizein, glycitein, or genistein contents were −0.1694, −0.1706, and −0.222, respectively, and between the protein and total isoflavone content, the coefficient was −0.2200. Therefore, there was a slight negative correlation between the protein content and that of isoflavones. Here is a significant test to show whether the coefficient is significantly different from 0.
- The research design is OK. Protein contents and isoflavone concentration vary across years dramatically. the Experiment design can be improved by conducting the experiment for at least 2 years.
Author Response
1. In row 91 to 92: The harvested soybean germplasms were stored in a -20°C freezer and used for protein and isoflavone analysis. What does germplasms mean here? Seeds, leaves, stems, or whole plants? Need clarify the term germplasm here.
Corrected it
2. In row 100: analysed by the micro-Kjeldahl method. Here needs a reference
Added a reference
3. In row 125: All data were subjected to analysis of variance (ANOVA) via the SAS software package. There was NO any report about the ANOVA analysis. The manuscript will be better if a ANOVA table reported in the main text to tell the interactions seed coar color, region, and size.
Corrected it
4. In row 228 to 231: The correlation coefficients for protein compared to daizein, glycitein, or genistein contents were −0.1694, −0.1706, and −0.222, respectively, and between the protein and total isoflavone content, the coefficient was −0.2200. Therefore, there was a slight negative correlation between the protein content and that of isoflavones. Here is a significant test to show whether the coefficient is significantly different from 0.
5. The research design is OK. Protein contents and isoflavone concentration vary across years dramatically. the Experiment design can be improved by conducting the experiment for at least 2 years.
Round 2
Reviewer 1 Report
Line 189-190: Mistake on 99,33%, must be 99.33%. The number of decimal should be the same trough the manuscript.
The amounts of protein content should be express as g 100g-1.
Line 140: Replace p-value <0.0001 by p <0.001, and p-value of less than 0.0001 by p< 0.001in all manuscript.
On legends of axis of figures, please, provide space before the units.
Footnote of Table 3 and Table 6 replace 100 seed weight: 24g< by 100 seed weight: <24g
Line 279: mistake in "difference".
Author Response
Line 189-190: Mistake on 99,33%, must be 99.33%. The number of decimal should be the same trough the manuscript.
Corrected it
The amounts of protein content should be express as g 100g-1.
Corrected it
Line 140: Replace p-value <0.0001 by p <0.001, and p-value of less than 0.0001 by p< 0.001in all manuscript.
Corrected it
On legends of axis of figures, please, provide space before the units.
Corrected it
Footnote of Table 3 and Table 6 replace 100 seed weight: 24g< by 100 seed weight: <24g
Corrected it
Line 279: mistake in "difference".
Corrected it
