Figure 1.
Effect of different media compositions on callus induction in terms of callus, number of days, and frequency in YT-53.
Figure 1.
Effect of different media compositions on callus induction in terms of callus, number of days, and frequency in YT-53.
Figure 2.
Callus of YT-53 on MS + 3 mg L −1 2,4-D + 0.5 mg L−1 Kinetin after 10 (a), 20 (b), and 30 (c) days of inoculation (2×).
Figure 2.
Callus of YT-53 on MS + 3 mg L −1 2,4-D + 0.5 mg L−1 Kinetin after 10 (a), 20 (b), and 30 (c) days of inoculation (2×).
Figure 3.
Effect of different media compositions on callus induction in terms of callus, number of days, and frequency in CP-774-00.
Figure 3.
Effect of different media compositions on callus induction in terms of callus, number of days, and frequency in CP-774-00.
Figure 4.
Callus of CP-77-400 on MS + 5 mg L−1 2,4-D after 10 (a), 20 (b), and 30 (c) days of inoculation (2×).
Figure 4.
Callus of CP-77-400 on MS + 5 mg L−1 2,4-D after 10 (a), 20 (b), and 30 (c) days of inoculation (2×).
Figure 5.
Effect of different media compositions on callus induction in terms of callus, number of days, and frequency in NSG-59.
Figure 5.
Effect of different media compositions on callus induction in terms of callus, number of days, and frequency in NSG-59.
Figure 6.
Callus of NSG-59 on MS + 4 mg L−1 2,4-D + 0.5 mg/L Kinetin after 10 (a), 20 (b), and 30 (c) days of inoculation (2×).
Figure 6.
Callus of NSG-59 on MS + 4 mg L−1 2,4-D + 0.5 mg/L Kinetin after 10 (a), 20 (b), and 30 (c) days of inoculation (2×).
Figure 7.
Effect of different media compositions on shoot induction in terms of shoot length, number of shoots, number of days, frequency in YT-53.
Figure 7.
Effect of different media compositions on shoot induction in terms of shoot length, number of shoots, number of days, frequency in YT-53.
Figure 8.
(a) Stereomicrograph showing shoot induction from callus culture in YT-53 on MS + 2 mg L−1 Kinetin (20×), (b) shoot induction in callus of YT-53 on MS + 2 mg L−1 Kinetin after 10 days (1×), and (c) shoot induction in callus YT-53 on MS + 2 mg L−1 Kinetin after 20 days of inoculation (1×).
Figure 8.
(a) Stereomicrograph showing shoot induction from callus culture in YT-53 on MS + 2 mg L−1 Kinetin (20×), (b) shoot induction in callus of YT-53 on MS + 2 mg L−1 Kinetin after 10 days (1×), and (c) shoot induction in callus YT-53 on MS + 2 mg L−1 Kinetin after 20 days of inoculation (1×).
Figure 9.
Effect of different media compositions on shoot induction in terms of shoot length, number of shoots, number of days, frequency in Cp-77400.
Figure 9.
Effect of different media compositions on shoot induction in terms of shoot length, number of shoots, number of days, frequency in Cp-77400.
Figure 10.
(a) Stereomicrograph showing shoot induction (arrows) from callus culture in CP-77-400 on MS + 5 mg L−1 2,4-D (20×), (b) shoot induction in callus of CP-77-400 on MS + 1 mg L−1 BAP after 10 days, and (c) shoot induction in callus of CP-77-400 on MS + 1 mg L−1 BAP after 20 days of inoculation (2×).
Figure 10.
(a) Stereomicrograph showing shoot induction (arrows) from callus culture in CP-77-400 on MS + 5 mg L−1 2,4-D (20×), (b) shoot induction in callus of CP-77-400 on MS + 1 mg L−1 BAP after 10 days, and (c) shoot induction in callus of CP-77-400 on MS + 1 mg L−1 BAP after 20 days of inoculation (2×).
Figure 11.
Effect of different media compositions on shoot induction in terms of shoot length (cm), number of shoots, frequency, and number of days in NSG-59.
Figure 11.
Effect of different media compositions on shoot induction in terms of shoot length (cm), number of shoots, frequency, and number of days in NSG-59.
Figure 12.
(a) Stereomicrograph showing shoot induction (arrows) from callus culture in NSG-59 on MS + 2 mg/L Kinetin (20×), (b) shoot induction in callus of NSG-59 on MS + 2 mg/L Kinetin after 10 days, and (c) shoot induction in callus of CP-77-400 on MS + 2 mg/L Kinetin after 20 days of inoculation (2×).
Figure 12.
(a) Stereomicrograph showing shoot induction (arrows) from callus culture in NSG-59 on MS + 2 mg/L Kinetin (20×), (b) shoot induction in callus of NSG-59 on MS + 2 mg/L Kinetin after 10 days, and (c) shoot induction in callus of CP-77-400 on MS + 2 mg/L Kinetin after 20 days of inoculation (2×).
Figure 13.
Effect of five treatments on root induction in terms of root length (cm), number of roots, rooting frequency and number of days in YT-53.
Figure 13.
Effect of five treatments on root induction in terms of root length (cm), number of roots, rooting frequency and number of days in YT-53.
Figure 14.
Effect of different media compositions on root induction in terms of root length (cm), number of roots, rooting frequency and number of days in CP-77400.
Figure 14.
Effect of different media compositions on root induction in terms of root length (cm), number of roots, rooting frequency and number of days in CP-77400.
Figure 15.
Effect of different media compositions on root induction in terms of root length (cm), number of roots, rooting frequency and number of days in NSG-59.
Figure 15.
Effect of different media compositions on root induction in terms of root length (cm), number of roots, rooting frequency and number of days in NSG-59.
Table 1.
Different Media compositions for callus induction.
Table 1.
Different Media compositions for callus induction.
T | Composition | T | Composition | T | Composition |
---|
T1 | Ms + 2,4-D (2.0 mg L−1) | T7 | MS + 2,4-D (2 mg L−1) + BAP (0.5 mg L−1) | T13 | MS + 2,4-D (4 mg L−1) + BAP (1 mg L−1) |
T2 | MS + 2,4-D (2.51 mg L−1) | T8 | MS + 2,4-D (3 mg L−1) + BAP (0.5 mg L−1) | T14 | MS + 2,4-D (5 mg L−1) + BAP (1 mg L−1) |
T3 | MS + 2,4-D (3 mg L−1) | T9 | MS + 2,4-D (4 mg L−1) + BAP (0.5 mg L−1) | T15 | MS + 2,4 D (2 mg L−1) + Kinetin (0.5 mg L−1) |
T4 | MS + 2,4-D (3.5 mg L−1) | T10 | MS + 2,4-D (5 mg L−1) + BAP (0.5 mg L−1) | T16 | MS + 2,4-D (3 mg L−1) + Kinetin (0.5 mg L−1) |
T5 | MS + 2,4-D (4 mg L−1) | T11 | MS + 2,4-D (2 mg L−1) + BAP (1 mg L−1) | T17 | MS + 2,4-D (4 mg L−1) + Kinetin (0.5 mg L−1) |
T6 | MS + 2,4-D (5 mg L−1) | T12 | MS + 2,4-D (3 mg L−1) + BAP (1 mg L−1) | T18 | MS + 2,4-D (5 mg L−1) + Kinetin (0.5 mg L−1) |
Table 2.
Different media compositions for shoot induction.
Table 2.
Different media compositions for shoot induction.
Treatment | Composition | Treatment | Composition |
---|
T1 | MS + BAP (1 mg L−1) | T7 | MS+ BAP (0.5 mg L−1) + Kinetin (0.5 mg L−1) |
T2 | MS + BAP (2 mg L−1) | T8 | MS + BAP (1 mg L−1) + Kinetin (1 mg L−1) |
T3 | MS + Kinetin (1 mg L−1) | T9 | MS + BAP (0.5 mg L−1) + IBA (0.5 mg L−1) |
T4 | MS + Kinetin (2 mg L−1) | T10 | MS + BAP (0.5 mg L−1) + IBA (0.5 mg L−1) + NAA (0.5 mg L−1) |
T5 | IBA (1 mg L−1) | T11 | MS + BAP (1 mg L−1) + IBA (1 mg L−1) + NAA (1 mg L−1) |
T6 | NAA (1 mg L−1) | T12 | MS + Kinetin (0.5 mg L−1) + IBA (0.5 mg L−1) + NAA (0.5 mg L−1) |
Table 3.
Media composition for root induction.
Table 3.
Media composition for root induction.
Treatment | Composition |
---|
T1 | 1/2 MS + NAA (1 mg L−1) |
T2 | 1/2 MS + NAA (2 mg L−1) |
T3 | 1/2 MS + NAA (3 mg L−1) |
T4 | 1/2 MS + NAA (1 mg L−1) + IBA (1 mg L−1) |
T5 | 1/2 MS + NAA (2 mg L−1) + IBA (2 mg L−1) |
Table 4.
Effect of treatments and genotypes and their interaction on callus induction.
Table 4.
Effect of treatments and genotypes and their interaction on callus induction.
Source of Variation | df | SS | % Variation Explained | MS | F | p-Value |
---|
Treatment | 17 | 2.389 | 41.724 | 0.141 | 34.960 | <0.001 |
Variety | 2 | 0.250 | 4.369 | 0.125 | 31.120 | <0.001 |
Treatment × Variety | 34 | 1.885 | 32.918 | 0.055 | 13.790 | <0.001 |
Error | 299 | 1.202 | 20.994 | 0.004 | | |
Total | 352 | 5.726 | | | | |
Grand Mean | 0.217 | CV% | 29.23 | R2 | 0.79 | |
Table 5.
Effect of treatments and genotypes and their interaction on shoot length (a) and number of shoots (b) during regeneration.
Table 5.
Effect of treatments and genotypes and their interaction on shoot length (a) and number of shoots (b) during regeneration.
(a) Source of Variation | df | SS | % Variation Explained | MS | F | p-Value |
---|
Treatment | 11 | 553.28 | 30.383 | 50.298 | 219.87 | <0.001 |
Variety | 2 | 205.38 | 11.278 | 102.689 | 448.88 | <0.001 |
Treatment × Variety | 22 | 1035.37 | 56.857 | 47.062 | 205.72 | <0.001 |
Error | 118 | 26.99 | 1.482 | 0.229 | | |
Total | 153 | 1821.02 | | | | |
Grand Mean | 1.682 | CV% | 28.44 | R2 | 0.985 | |
(b) Treatment | 11 | 2507.73 | 50.095 | 227.976 | 135.54 | <0.001 |
Variety | 2 | 229.13 | 4.577 | 114.563 | 68.11 | <0.001 |
Treatment × Variety | 22 | 2070.59 | 41.363 | 94.118 | 55.96 | <0.001 |
Error | 118 | 198.48 | 3.965 | 1.682 | | |
Total | 153 | 5005.93 | | | | |
Grand Mean | 6.76 | CV% | 19.19 | R2 | 0.960 | |
Table 6.
Effect of treatments and genotypes on root length (a) and number of roots (b) during the rooting process.
Table 6.
Effect of treatments and genotypes on root length (a) and number of roots (b) during the rooting process.
(a) Source of Variation | df | SS | % Variation Explained | MS | F | p-Value |
---|
treat | 4 | 0.70979 | 5.208 | 0.17745 | 2.32 | 0.066 |
Variety | 2 | 1.02792 | 7.542 | 0.51396 | 6.71 | 0.0022 |
Treatment × Variety | 8 | 6.76283 | 49.618 | 0.84535 | 11.04 | <0.001 |
Error | 67 | 5.12912 | 37.632 | 0.07655 | | |
Total | 81 | 13.6297 | | | | |
Grand Mean | 0.671 | CV% | 36.37 | R2 | 0.624 | |
(b) Treatment | 4 | 280.86 | 14.300 | 70.215 | 47.11 | <0.001 |
Variety | 2 | 1113.03 | 56.669 | 556.517 | 373.43 | <0.001 |
Treatment × Variety | 8 | 470.36 | 23.948 | 58.795 | 39.45 | <0.001 |
Error | 67 | 99.85 | 5.084 | 1.49 | | |
Total | 81 | 1964.1 | | | | |
Grand Mean | 9.25 | CV% | 13.2 | R2 | 0.949 | |