Lipopeptides Produced by Bacillus mojavensis P1709 as an Efficient Tool to Maintain Postharvest Cherry Tomato Quality and Quantity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Biosurfactant Producing Bacteria
2.2. Emulsification Index Assessment
2.3. Identification of Genes Responsible for Lipopeptide Synthesis
2.4. Biosurfactant Production, Extraction and Purification
2.5. Chemical Characterization of the APF
2.5.1. Thin Layer Chromatography (TLC) with Following Staining
2.5.2. Fourier Transform Infrared Spectroscopy (FTIR)
2.6. Effects of Biosurfactants on Radial Fungal Growth and Mycotoxin Production In Vitro
2.7. Effects of Biosurfactants on Fungal Growth on Cherry Tomatoes In Vivo
2.8. Effects of Biosurfactants on Defense-Related Enzymes of Cherry Tomatoes
2.9. Statistical Analysis
3. Results and Discussion
3.1. Emulsifying Ability of B. mojavensis P1709
3.2. Antimicrobial Peptide Synthesis Genes
3.3. TLC and FTIR Analysis
3.4. Effects of Biosurfactants on Radial Fungal Growth and Mycotoxin Production In Vitro
3.5. Effects of APF on Fungal Growth on Cherry Tomatoes In Vivo
3.6. Effects of Biosurfactants on Defense-Related Enzymes of Cherry Tomatoes
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Target Gene | Primer Sequence, 5′-3′ | Amplicon Length (bp) |
---|---|---|
fenD | F: GGCCCGTTCTCTAAATCCAT | 670 |
R: GTCATGCTGACGAGAGCAAA | ||
ituC | F: GGCTGCTGCAGATGCTTTAT | 423 |
R: TCGCAGATAATCGCAGTGAG | ||
srfAA | F: TCGGGACAGGAAGACATCAT | 201 |
R: CCACTCAAACGGATAATCCTGA |
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Galitskaya, P.; Karamova, K.; Biktasheva, L.; Galieva, G.; Gordeev, A.; Selivanovskaya, S. Lipopeptides Produced by Bacillus mojavensis P1709 as an Efficient Tool to Maintain Postharvest Cherry Tomato Quality and Quantity. Agriculture 2022, 12, 609. https://doi.org/10.3390/agriculture12050609
Galitskaya P, Karamova K, Biktasheva L, Galieva G, Gordeev A, Selivanovskaya S. Lipopeptides Produced by Bacillus mojavensis P1709 as an Efficient Tool to Maintain Postharvest Cherry Tomato Quality and Quantity. Agriculture. 2022; 12(5):609. https://doi.org/10.3390/agriculture12050609
Chicago/Turabian StyleGalitskaya, Polina, Kamalya Karamova, Liliya Biktasheva, Gulnaz Galieva, Alexander Gordeev, and Svetlana Selivanovskaya. 2022. "Lipopeptides Produced by Bacillus mojavensis P1709 as an Efficient Tool to Maintain Postharvest Cherry Tomato Quality and Quantity" Agriculture 12, no. 5: 609. https://doi.org/10.3390/agriculture12050609