Infection Biology of Stagonosporopsis cucurbitacearum in Watermelon and Defence Responses in the Host
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plants
2.2. Inoculum of Stagonosporopsis cucurbitacearum and Inoculation
2.3. Leaf Infection
2.4. Stem Infection
2.5. Infection Biology and Accumulation of H2O2
2.6. Protein Extraction for Studies of Peroxidase and β-1,3-Glucanase
2.7. Peroxidase
2.8. β-1,3-Glucanase
2.9. Native PAGE for Detection of Peroxidase Isoforms
2.10. Experimental Design and Statistical Analysis of Data
3. Results
3.1. Disease Development
3.2. Infection Biology and Accumulation of H2O2 at Penetration Sites in Leaves
3.3. Peroxidase Activity
3.4. Peroxidase Isoforms
3.5. β-1,3-Glucanase Activity
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Accession | Percentage Leaf Area with Symptoms 4 dai | Length of Stem Lesion (mm) | |
---|---|---|---|
4 dai | 9 dai | ||
PI189225 | 4.0 | 2.9 | 6.7 |
232-0125/B | 58.9 | 4.9 | 10.3 |
LSD95 | 22.6 | 0.7 | 0.8 |
p-value | 0.0002 | 0.0001 | 0.0001 |
Percentage of | Time after Inoculation with Stagonosporopsis cucurbitacearum | ||||||||
---|---|---|---|---|---|---|---|---|---|
14 hai | 20 hai | 44 hai | |||||||
R | S | R | S | R | S | ||||
spores forming appressoria | 4.6 | 5.0 | ns | 13.8 | 17.1 | ns | - b | - b | |
appressoria causing penetration | - a | - a | - | 0.0 | 9.8 | ** | 15.5 | 22.5 | * |
appressoria with successful penetrations with tissue colonisation | - a | - a | - | - a | - a | - | 4.5 | 8.0 | *** |
appressoria with H2O2 accumulation | 54.2 | 0.0 | *** | 39.8 | 33.0 | ns | 26.0 | 28.0 | ns |
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Nga, N.T.T.; de Neergaard, E.; Jørgensen, H.J.L. Infection Biology of Stagonosporopsis cucurbitacearum in Watermelon and Defence Responses in the Host. Agriculture 2024, 14, 380. https://doi.org/10.3390/agriculture14030380
Nga NTT, de Neergaard E, Jørgensen HJL. Infection Biology of Stagonosporopsis cucurbitacearum in Watermelon and Defence Responses in the Host. Agriculture. 2024; 14(3):380. https://doi.org/10.3390/agriculture14030380
Chicago/Turabian StyleNga, Nguyen Thi Thu, Eigil de Neergaard, and Hans Jørgen Lyngs Jørgensen. 2024. "Infection Biology of Stagonosporopsis cucurbitacearum in Watermelon and Defence Responses in the Host" Agriculture 14, no. 3: 380. https://doi.org/10.3390/agriculture14030380