Identification and Fine Mapping of Quantitative Trait Loci for Tiller Angle Using Chromosome Segment Substitution Lines in Rice (Oryza Sativa L.)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Evaluation of the Tiller Angle
2.3. DNA Extraction, PCR, and Gene Sequencing
2.4. Identification of Quantitative Trait Loci
2.5. RNA Isolation and Quantitative Reverse Transcription PCR
2.6. Statistical Analysis
3. Results
3.1. Phenotypic Variations in the Tiller Angle in the Chromosome Segment Substitution Lines
3.2. Quantitative Trait Locus Analysis for the Tiller Angle
3.3. Fine Mapping of qTA9-2 and Analysis of Candidate Genes
3.4. Potential Utilization of CSSLs to Improve Rice Architecture
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Location | Parents (Mean ± SD) | CSSLs | ||||||
---|---|---|---|---|---|---|---|---|
Koshihikari | Nona Bokra | p Value | Mean ± SD | Range | Skewness | Kurtosis | p Value a | |
Lingshui | 6.1 ± 0.64 | 13.1 ± 2.37 | <0.0001 | 6.3 ± 2.68 | 1.49~15.05 | 1.09 | 1.80 | 0.065 |
Yangzhou | 5.2 ± 0.98 | 16.3 ± 2.2 | <0.0001 | 8.3 ± 3.05 | 1.85~18.38 | 0.41 | 0.51 | 0.333 |
QTL | Chr. | Marker Interval | Position (cM) | LOD | Additive Effect | PVE (%) | |||
---|---|---|---|---|---|---|---|---|---|
Lingshui | Yangzhou | Lingshui | Yangzhou | Lingshui | Yangzhou | ||||
qTA1 | 1 | RM11053–RM11268 | 82.7~99.1 | 2.64 | — | 0.06 | — | 4.12 | — |
qTA5 | 5 | RM18933–RM19044 | 99.0~108.2 | — | 2.97 | — | −1.34 | — | 3.78 |
qTA9-1 | 9 | RM24104–RM24199 | 47.3~53.7 | — | 5.52 | — | 1.93 | — | 7.12 |
qTA9-2 | 9 | RM24350–RM24393 | 64.3~66.5 | 7.56 | 3.04 | 2.32 | 0.62 | 8.06 | 5.82 |
qTA11 | 11 | RM26002–STS11-2 | 3.6~23.6 | — | 2.71 | — | −0.78 | — | 8.22 |
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Leng, Y.; Tao, T.; Lu, S.; Liu, R.; Yang, Q.; Zhang, M.; Hong, L.; Guo, Q.; Ren, X.; Yang, Z.; et al. Identification and Fine Mapping of Quantitative Trait Loci for Tiller Angle Using Chromosome Segment Substitution Lines in Rice (Oryza Sativa L.). Agriculture 2024, 14, 1002. https://doi.org/10.3390/agriculture14071002
Leng Y, Tao T, Lu S, Liu R, Yang Q, Zhang M, Hong L, Guo Q, Ren X, Yang Z, et al. Identification and Fine Mapping of Quantitative Trait Loci for Tiller Angle Using Chromosome Segment Substitution Lines in Rice (Oryza Sativa L.). Agriculture. 2024; 14(7):1002. https://doi.org/10.3390/agriculture14071002
Chicago/Turabian StyleLeng, Yujia, Tao Tao, Shuai Lu, Ran Liu, Qingqing Yang, Mingqiu Zhang, Lianmin Hong, Qianqian Guo, Xinzhe Ren, Zhidi Yang, and et al. 2024. "Identification and Fine Mapping of Quantitative Trait Loci for Tiller Angle Using Chromosome Segment Substitution Lines in Rice (Oryza Sativa L.)" Agriculture 14, no. 7: 1002. https://doi.org/10.3390/agriculture14071002