Wolbachia Transinfection and Effect on the Biological Traits of Matsumuratettix hiroglyphicus (Matsumura), the Leafhopper Vector of Sugarcane White Leaf Disease
Abstract
:1. Introduction
2. Materials and Methods
2.1. Collection and Mass Rearing of the Leafhoppers
2.2. Preparation of Wolbachia Source
2.3. Microinjection for Wolbachia Transinfection
2.4. Wolbachia Infection Dynamics
2.5. DNA Extraction and PCR Amplification
2.6. Wolbachia Quantification
2.7. Wolbachia Distribution in Dissected Organs
2.8. Fluorescence In Situ Hybridization (FISH)
2.9. Determining the Effect of Wolbachia Transinfection on Development Time and Adult Emergence
2.10. Effect of Wolbachia Transinfection on Adult Survival and Longevity
2.11. Crossing Experiment
2.12. Statistical Analysis
3. Results
3.1. Wolbachia Transinfection
3.2. Wolbachia Infection Frequency in the Transinfected M. hiroglyphicus Samples
3.3. Distribution of Wolbachia in the Transinfected M. hiroglyphicus Samples
3.4. Effect of Wolbachia Transinfection on the Development Time of the Immature Stages
3.5. Effect of Wolbachia Transinfection on Adult Survival
3.6. Effect of Wolbachia Infection on Fecundity and CI Expression
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Experiment | No. of Injected Nymphs/ No. of Surviving Nymphs (%) | No. of Surviving Nymphs/ No. of Females Emerged (G0) (%) | No. of G0 Female/No. of Wolbachia-Infected (%) | No. of Injected Nymphs/ /No. of Wolbachia-Infected Female (%) |
---|---|---|---|---|
1 | 50/15 (30.00%) | 15/5 (33.33%) | 5/4 (80.00%) | 50/4 (8.00%) |
2 | 50/21 (42.00%) | 21/8 (38.09%) | 8/4 (50.00%) | 50/4 (8.00%) |
3 | 50/17 (34.00%) | 17/6 (35.29%) | 6/3 (50.00%) | 50/3 (8.00%) |
4 | 50/22 (44.00%) | 22/7 (31.82%) | 7/5 (71.43%) | 50/5 (10.00%) |
Total | 200/75 (37.50%) | 75/26 (34.67%) | 26/16 (61.54%) | 200/16 (8.00%) |
Developmental Stage (G1) | Infection Frequency of Wolbachia (% ± Standard Error) * |
---|---|
Egg | 62.00 ± 3.74 |
1st nymph | 60.00 ± 4.47 |
2nd nymph | 70.00 ± 7.07 |
3rd nymph | 58.00 ± 4.90 |
4th nymph | 68.00 ± 3.74 |
5th nymph | 64.00 ± 5.10 |
Generation | Wolbachia Infection Frequency (% ± Standard Error) * |
---|---|
G1 | 64.00 ± 2.44 ab |
G2 | 72.50 ± 4.28 a |
G3 | 80.00 ± 8.16 a |
G4 | 67.50 ± 4.28 ab |
G5 | 72.50 ± 5.63 a |
G6 | 62.50 ± 2.24 ab |
G7 | 55.00 ± 5.77 b |
G8 | 62.50 ± 2.24 ab |
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Suwanchaisri, K.; Roddee, J.; Wangkeeree, J. Wolbachia Transinfection and Effect on the Biological Traits of Matsumuratettix hiroglyphicus (Matsumura), the Leafhopper Vector of Sugarcane White Leaf Disease. Agriculture 2024, 14, 1236. https://doi.org/10.3390/agriculture14081236
Suwanchaisri K, Roddee J, Wangkeeree J. Wolbachia Transinfection and Effect on the Biological Traits of Matsumuratettix hiroglyphicus (Matsumura), the Leafhopper Vector of Sugarcane White Leaf Disease. Agriculture. 2024; 14(8):1236. https://doi.org/10.3390/agriculture14081236
Chicago/Turabian StyleSuwanchaisri, Kamonrat, Jariya Roddee, and Jureemart Wangkeeree. 2024. "Wolbachia Transinfection and Effect on the Biological Traits of Matsumuratettix hiroglyphicus (Matsumura), the Leafhopper Vector of Sugarcane White Leaf Disease" Agriculture 14, no. 8: 1236. https://doi.org/10.3390/agriculture14081236