Effects of Nitrogen Deposition and Precipitation Patterns on Nitrogen Allocation of Mongolian Pine (Pinus sylvestris var. mongolica) on Sandy Land Using 15N Isotope
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Materials
2.2. Experimental Design
2.3. Measurement and Calculation of Indicators
2.3.1. Sample Collection and Dry Weight Measurement
2.3.2. N Content and 15N Abundance Measurement
2.3.3. Calculation
2.4. Data Processing
3. Results
3.1. Impact of Nitrogen Deposition and Precipitation Patterns on 15N Abundance in Mongolian Pine
3.1.1. Changes in 15N Abundance in Seedlings
3.1.2. Changes in Soil 15N Abundance
3.2. Correlation and Variance Analysis of 15N Abundance in Different Organs and Soil of Mongolian Pine Seedlings Under Various Treatments of Nitrogen Deposition and Precipitation Patterns
3.2.1. Correlation Analysis
3.2.2. Analysis of Variance
3.3. Effects of Nitrogen Deposition and Changes in Precipitation Patterns on 15N Uptake by Mongolian Pine
3.3.1. Ndff (%) in Different Organs of Mongolian Pine Seedlings and Soil
3.3.2. Nitrogen Content and 15N Absorption
3.4. Effects of Nitrogen Deposition and Precipitation Patterns on the 15N Distribution Ratio in Organs of Mongolian Pine
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Month | Organs | Treatments | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
WCNC | WINC | WDNC | WCNL | WINL | WDNL | WCNH | WINH | WDNH | ||
August | Root | 0.03 ± 0.00 A c | 0.03 ± 0.00 A c | 0.05 ± 0.00 A c | 2.89 ± 0.01 B b | 4.19 ± 0.03 A b | 2.37 ± 0.00 C b | 2.78 ± 0.01 C a | 5.53 ± 0.11 A a | 3.97 ± 0.04 B a |
Stem | 0.08 ± 0.01 A c | 0.05 ± 0.00 A c | 0.07 ± 0.01 A c | 4.11 ± 0.02 B b | 4.45 ± 0.03 A b | 2.93 ± 0.06 C b | 4.02 ± 0.06 C a | 6.56 ± 0.04 A a | 5.08 ± 0.02 B b | |
Leaf | 0.02 ± 0.00 A c | 0.07 ± 0.00 A c | 0.02 ± 0.02 A c | 3.45 ± 0.01 B b | 3.61 ± 0.01 A b | 2.46 ± 0.03 C b | 3.83 ± 0.04 C a | 6.70 ± 0.04 A a | 5.04 ± 0.22 B a | |
October | Root | 0.28 ± 0.01 A c | 0.21 ± 0.01 A c | 0.29 ± 0.01 A c | 4.71 ± 0.04 B b | 5.49 ± 0.06 A b | 3.72 ± 0.00 C b | 5.16 ± 0.02 B a | 7.93 ± 0.12 A a | 3.59 ± 0.18 C a |
Stem | 0.20 ± 0.02 A c | 0.21 ± 0.00 A c | 0.25 ± 0.00 A c | 4.88 ± 0.01 B b | 5.58 ± 0.03 A b | 3.89 ± 0.02 C b | 5.82 ± 0.07 B a | 7.59 ± 0.06 A a | 3.78 ± 0.08 C a | |
Leaf | 0.03 ± 0.00 A c | 0.05 ± 0.00 A c | 0.09 ± 0.00 A c | 4.30 ± 0.13 B b | 5.53 ± 0.01 A b | 3.57 ± 0.07 C a | 5.20 ± 0.01 B a | 8.13 ± 0.01 A a | 3.55 ± 0.03 C a |
M | W | N | M × W | M × N | W × N | M × W × N | |
---|---|---|---|---|---|---|---|
Root | ** | ** | ** | ** | ** | ** | ** |
Stem | ** | ** | ** | ** | ** | ** | ** |
Leaf | ** | ** | ** | ** | ** | ** | ** |
Soil | ** | ** | ** | ** | ** | ** | ** |
Month | Treatments | Nitrogen Content in Organs (mg/plant) | 15N Absorption in Organs (mg/plant) | ||||
---|---|---|---|---|---|---|---|
Root | Stem | Leaf | Root | Stem | Leaf | ||
August | WCNL | 3.33 ± 0.76 A a | 3.93 ± 0.59 B a | 16.99 ± 4.48 A a | 0.86 ± 0.20 B a | 1.50 ± 0.23 B a | 5.35 ± 1.41 A a |
WINL | 2.98 ± 0.53 A a | 5.23 ± 1.23 A b | 19.45 ± 4.15 A a | 1.17 ± 0.21 A a | 2.18 ± 0.51 A b | 6.45 ± 1.38 A b | |
WDNL | 2.38 ± 1.26 A a | 2.92 ± 1.14 B a | 10.37 ± 3.28 B b | 0.49 ± 0.26 C b | 0.77 ± 0.30 C b | 2.23 ± 0.70 B b | |
WCNH | 2.73 ± 1.10 A a | 3.91 ± 1.25 B a | 13.84 ± 2.88 B a | 0.67 ± 0.27 B a | 1.46 ± 0.47 B a | 4.91 ± 1.02 C a | |
WINH | 2.76 ± 0.60 A a | 7.18 ± 1.26 A a | 21.86 ± 4.44 A a | 1.46 ± 0.32 A a | 4.55 ± 0.79 A a | 14.17 ± 2.88 A a | |
WDNH | 2.74 ± 1.12 A a | 3.34 ± 0.70 B a | 20.08 ± 5.91 A a | 1.01 ± 0.41 B a | 1.61 ± 0.34 B a | 9.61 ± 2.83 B a | |
October | WCNL | 5.35 ± 1.89 A a | 7.40 ± 3.01 A a | 30.39 ± 4.14 A a | 2.38 ± 0.84 AB a | 2.73 ± 1.11 A a | 11.2 ± 1.52 A a |
WINL | 5.18 ± 3.56 A a | 5.14 ± 2.31 AB a | 25.97 ± 12.87 A a | 2.72 ± 1.87 A a | 2.74 ± 1.23 A b | 13.73 ± 6.80 A a | |
WDNL | 2.51 ± 1.36 A a | 4.34 ± 0.70 B a | 13.19 ± 4.04 B a | 0.86 ± 0.47 B a | 1.56 ± 0.25 A a | 4.32 ± 1.32 B a | |
WCNH | 2.14 ± 1.15 B b | 5.57 ± 0.46 A a | 14.43 ± 3.86 B b | 1.05 ± 0.56 B b | 3.11 ± 0.26 B a | 7.13 ± 1.91 B b | |
WINH | 8.83 ± 6.63 A a | 7.72 ± 3.11 A a | 27.65 ± 9.65 A a | 6.84 ± 5.13 A a | 5.70 ± 2.30 A a | 21.96 ± 7.66 A a | |
WDNH | 4.00 ± 3.02 AB a | 5.19 ± 1.70 A a | 17.38 ± 7.45 B a | 1.32 ± 1.00 B a | 1.81 ± 0.59 B a | 5.66 ± 2.42 B a |
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Zhao, T.; Cheng, S.; Gang, Q.; Zhuang, Y.; Zhu, X. Effects of Nitrogen Deposition and Precipitation Patterns on Nitrogen Allocation of Mongolian Pine (Pinus sylvestris var. mongolica) on Sandy Land Using 15N Isotope. Agriculture 2024, 14, 1367. https://doi.org/10.3390/agriculture14081367
Zhao T, Cheng S, Gang Q, Zhuang Y, Zhu X. Effects of Nitrogen Deposition and Precipitation Patterns on Nitrogen Allocation of Mongolian Pine (Pinus sylvestris var. mongolica) on Sandy Land Using 15N Isotope. Agriculture. 2024; 14(8):1367. https://doi.org/10.3390/agriculture14081367
Chicago/Turabian StyleZhao, Tianhong, Shihao Cheng, Qun Gang, Yonghui Zhuang, and Xianjin Zhu. 2024. "Effects of Nitrogen Deposition and Precipitation Patterns on Nitrogen Allocation of Mongolian Pine (Pinus sylvestris var. mongolica) on Sandy Land Using 15N Isotope" Agriculture 14, no. 8: 1367. https://doi.org/10.3390/agriculture14081367