Combined Transcriptome and Metabolome Analysis of Alfalfa Responses to Aphid Infestation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Material Preparation, Alfalfa Treatment, and Sample Collection
2.2. RNA Extraction, Library Construction, and Sequencing
2.3. Functional Annotation and Differentially Expressed Gene Identification
2.4. Extraction of Metabolites
2.5. LC-MS/MS Analysis
2.6. Metabolome Data Preprocessing, Annotation, and Analysis
2.7. Comprehensive Analysis of Transcriptome and Metabolome
2.8. Statistical Analysis
3. Result
3.1. Analysis of Differentially Expressed Genes in Alfalfa Plants Infested with Aphids
3.2. Weighted Gene Co-Expression Network Analysis (WGCNA)
3.3. Analysis of Differentially Accumulated Metabolites in Alfalfa Infested with Aphids
3.4. Analysis of DEGs and DAMs of the Phenylpropanoid Biosynthesis Pathway
3.5. Analysis of DEGs and DAMs Involved in Flavonoid Biosynthesis
3.6. Analysis of DEGs and DAMs Involved in Isoflavonoid Biosynthesis
4. Discussion
4.1. Transcriptome Analysis
4.2. Metabolome Analysis
4.3. Phenylpropanoid Biosynthesis
4.4. Flavonoid Biosynthesis and Isoflavonoid Biosynthesis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, H.; Xu, M.; Guo, Y.; Dan, Z.; Liu, X.; Zhang, J.; Li, C.; Jia, S.; Jia, L.; Yu, A.; et al. Combined Transcriptome and Metabolome Analysis of Alfalfa Responses to Aphid Infestation. Agriculture 2024, 14, 1545. https://doi.org/10.3390/agriculture14091545
Liu H, Xu M, Guo Y, Dan Z, Liu X, Zhang J, Li C, Jia S, Jia L, Yu A, et al. Combined Transcriptome and Metabolome Analysis of Alfalfa Responses to Aphid Infestation. Agriculture. 2024; 14(9):1545. https://doi.org/10.3390/agriculture14091545
Chicago/Turabian StyleLiu, Hao, Ming Xu, Yuhan Guo, Zhencuo Dan, Xin Liu, Jiayi Zhang, Cong Li, Shizhen Jia, Lei Jia, Ailing Yu, and et al. 2024. "Combined Transcriptome and Metabolome Analysis of Alfalfa Responses to Aphid Infestation" Agriculture 14, no. 9: 1545. https://doi.org/10.3390/agriculture14091545