Surface-Modified Silver Nanoparticles and Their Encapsulation in Liposomes Can Treat MCF-7 Breast Cancer Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Reagents
2.2. Silver Nanoparticles Synthesis
2.3. PVP-AgNPs Synthesis
2.4. BSA-AgNPs Synthesis
2.5. Liposomal Preparation and Encapsulation of AgNPs
2.6. Characterisation of AgNPs
2.6.1. UV-Visible Spectrophotometry
2.6.2. Particle Size Distribution and Zeta Potential Analysis
2.6.3. Fluorescence Analysis
2.6.4. Morphological Analysis Using Scanning Electron Microscopy (SEM)
2.7. Cell Culture and Exposure
2.8. Cell Viability
2.9. Statistical Analysis
3. Results and Discussion
3.1. UV–Visible Spectrophotometer Analysis of AgNPs and Liposomes
3.2. Fluorescence Analysis of the BSA-AgNPs
3.3. Particle Size Distribution Analysis and Zeta Potential of AgNPs and Liposomes
3.4. Scanning Electron Microscopic Analysis of AgNPs and Liposomes
3.5. Cytotoxicity Analysis of AgNPs and Liposomes
3.6. Unmodified AgNPs
3.7. BSA-AgNPs
3.8. PVP-AgNPs
3.9. Liposomal-AgNPs
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Colloidal Sample | Zeta Potential (mV) |
---|---|
AgNPs (NaBH4) | −41.33 |
AgNPs (NaBH4 and Na3C6H5O7) | −0.73 |
PVP-AgNPs | −33.0 |
Liposomal-AgNPs | −30.66 |
BSA 100 mg | −19.66 |
BSA 50 mg | 18.46 |
BSA 25 mg | −0.07 |
BSA 12.5 mg | 1.75 |
Sample | Concentration (μL/mL) | |||||||
---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | |
AgNPs (NaBH4) | 59.5% | 63% | 43% | 33.6% | 32.6% | 23.3% | 24.4% | 31.5% |
PVP-AgNPs | 67.9% | 60.4% | 63.4% | 40.7% | 37% | 27.3% | 22.1% | 21.4% |
BSA-AgNPs | 18.4% | 12.6% | 11.1% | 11.3% | 7.7% | 6.4% | 8.5% | 5.9% |
Liposomal-AgNPs | 90.6% | 80.5% | 87% | 79.5% | 53.8% | 24.1% | 13.9% | 17% |
AgNO3 | 31.8% | 19.8% | 8.6% | 2.95% | 0.6% | 0.38% | 0.25% | 0.23% |
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Moors, E.; Sharma, V.; Tian, F.; Javed, B. Surface-Modified Silver Nanoparticles and Their Encapsulation in Liposomes Can Treat MCF-7 Breast Cancer Cells. J. Funct. Biomater. 2023, 14, 509. https://doi.org/10.3390/jfb14100509
Moors E, Sharma V, Tian F, Javed B. Surface-Modified Silver Nanoparticles and Their Encapsulation in Liposomes Can Treat MCF-7 Breast Cancer Cells. Journal of Functional Biomaterials. 2023; 14(10):509. https://doi.org/10.3390/jfb14100509
Chicago/Turabian StyleMoors, Ellenor, Vinayak Sharma, Furong Tian, and Bilal Javed. 2023. "Surface-Modified Silver Nanoparticles and Their Encapsulation in Liposomes Can Treat MCF-7 Breast Cancer Cells" Journal of Functional Biomaterials 14, no. 10: 509. https://doi.org/10.3390/jfb14100509
APA StyleMoors, E., Sharma, V., Tian, F., & Javed, B. (2023). Surface-Modified Silver Nanoparticles and Their Encapsulation in Liposomes Can Treat MCF-7 Breast Cancer Cells. Journal of Functional Biomaterials, 14(10), 509. https://doi.org/10.3390/jfb14100509