Cationic Serine-Based Gemini Surfactant:Monoolein Aggregates as Viable and Efficacious Agents for DNA Complexation and Compaction: A Cytotoxicity and Physicochemical Assessment
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Sample Preparation
2.3. Cell Culture and Cytotoxicity Evaluation
2.4. Light Microscopy
2.5. Dynamic Light Scattering (DLS)
2.6. DNA Complexation: Ethidium Bromide Exclusion Assay
3. Results and Discussion
3.1. Interactions between Gemini:MO Aggregates and DNA
3.2. Characterization of the Gemini:MO Aggregation Behavior
3.3. Discussion of the Main Interaction/Aggregation Trends
3.4. Cytotoxicity of the Gemini–MO Systems
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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System | Gemini:MO Molar Ratio | IC50/µM |
---|---|---|
(12Ser)2N5:MO | 2:1 | 35 ± 5 |
1:1 | 50 ± 5 | |
1:2 | 62 ± 9 | |
1:4 | 64 ± 5 | |
(14Ser)2N5:MO | 2:1 | 45 ± 6 |
1:1 | 51 ± 8 | |
1:2 | 62 ± 7 | |
1:4 | 55 ± 8 | |
(16Ser)2N5:MO | 2:1 | 64 ± 3 |
1:1 | 72 ± 3 | |
1:2 | 76 ± 7 | |
1:4 | 90 ± 7 | |
(18Ser)2N5:MO | 2:1 | >100 |
1:1 | 43 ± 7 | |
1:2 | 46 ± 8 | |
1:4 | 74 ± 8 |
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Oliveira, I.S.; Silva, S.G.; Gomes, A.C.; Real Oliveira, M.E.C.D.; Vale, M.L.C.d.; Marques, E.F. Cationic Serine-Based Gemini Surfactant:Monoolein Aggregates as Viable and Efficacious Agents for DNA Complexation and Compaction: A Cytotoxicity and Physicochemical Assessment. J. Funct. Biomater. 2024, 15, 224. https://doi.org/10.3390/jfb15080224
Oliveira IS, Silva SG, Gomes AC, Real Oliveira MECD, Vale MLCd, Marques EF. Cationic Serine-Based Gemini Surfactant:Monoolein Aggregates as Viable and Efficacious Agents for DNA Complexation and Compaction: A Cytotoxicity and Physicochemical Assessment. Journal of Functional Biomaterials. 2024; 15(8):224. https://doi.org/10.3390/jfb15080224
Chicago/Turabian StyleOliveira, Isabel S., Sandra G. Silva, Andreia C. Gomes, M. Elisabete C. D. Real Oliveira, M. Luísa C. do Vale, and Eduardo F. Marques. 2024. "Cationic Serine-Based Gemini Surfactant:Monoolein Aggregates as Viable and Efficacious Agents for DNA Complexation and Compaction: A Cytotoxicity and Physicochemical Assessment" Journal of Functional Biomaterials 15, no. 8: 224. https://doi.org/10.3390/jfb15080224