An Evaluation of the Cytocompatibility of Endodontic Bioceramics in Human Periodontal-Ligament-Derived Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Human Periodontal-Ligament-Derived Cells (hPDLCs)
2.2. Preparation of Bioceramic Discs
2.3. Cytocompatibility
2.4. Effects of the Powder–Liquid (Paste) Ratio of F or BG on Cytocompatibility
2.5. Statistical Analyses
2.6. Sample Size
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Materials | Manufactures | Composition |
---|---|---|
MTA Repair HP | Angelus (Brazil) | Powder: calcium silicate, calcium aluminate, calcium oxide, calcium tungstate Liquid: purified water, plasticizer |
MTA Flow White | Ultradent Products (USA) | Powder: tricalcium silicate, dicalcium silicate, calcium sulfate, etc. Gel: purified water, thickening agent |
Nishika Canal Sealer BG Multi | Nippon Shika Yakuhin (Japan) | Paste A: fatty acids, bismuth bicarbonate, silicon dioxide Paste B: magnesium oxide, purified water, calcium silicate glass, silicon dioxide, etc. Powder: calcium silicate glass, calcium hydroxide |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | Mean (SD) | |
---|---|---|---|---|---|---|---|---|---|---|---|
HP | 0.91 | 1.06 | 1.57 | 1.81 | 1.67 | 1.59 | 1.17 | 0.83 | 1.32 | 1.44 | 1.34 (0.33) a |
BG | 0.15 | 0.41 | 0.43 | 0.63 | 0.76 | 0.15 | 0.27 | 0.33 | 0.27 | 0.43 | 0.38 (0.20) b |
F | −0.01 | 0.01 | 0.06 | 0.02 | 0.01 | 0.03 | 0.02 | 0.01 | 0.03 | 0.06 | 0.03 (0.02) b |
NC | 1.28 | 1.13 | 1.27 | 1.90 | 1.54 | 1.67 | 1.05 | 0.96 | 1.92 | 1.22 | 1.39 (0.34) a |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | Mean (SD) | |
---|---|---|---|---|---|---|---|---|---|---|---|
HP | 3.12 | 3.16 | 2.93 | 2.81 | 2.85 | 2.66 | 2.15 | 2.52 | 3.18 | 3.28 | 2.87 (0.35) a |
BG | −0.08 | 2.22 | 1.38 | 2.42 | 1.90 | 1.21 | 1.34 | 0.85 | 1.94 | 2.62 | 1.58 (0.81) b |
F | 0.01 | 0.02 | −0.01 | 0.01 | 0.02 | 0.01 | 0.01 | 0.01 | 0.04 | 2.13 | 0.23 (0.67) c |
NC | 2.47 | 2.99 | 2.62 | 2.79 | 2.45 | 2.45 | 2.45 | 2.39 | 3.07 | 3.08 | 2.64 (0.32) a |
7 Days | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | Mean (SD) | p-Value | |
F0 | 0.03 | 0.02 | 0.01 | 0.05 | 0.07 | 0.22 | 0.01 | 0.18 | 0.22 | 0.06 | 0.14 | 0.09 (0.08) | reference |
F1 | 0.00 | 0.23 | 1.22 | 0.67 | 0.04 | 0.05 | 0.06 | 0.10 | 0.04 | 0.22 | 0.25 | 0.26 (0.37) | 0.165 |
F2 | 0.01 | 1.49 | 1.72 | 0.45 | 1.35 | 0.08 | 0.75 | 0.26 | 0.07 | 1.80 | 0.06 | 0.73 (0.72) | 0.015 |
NC | 1.37 | 1.20 | 1.70 | 1.54 | 1.58 | 0.90 | 1.26 | 1.05 | 0.90 | 1.21 | 1.41 | 1.28 (0.27) | <0.001 |
28 Days | |||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | Mean (SD) | p-Value | |
F0 | 2.34 | 0.03 | 0.16 | 1.32 | 2.67 | 0.55 | 0.39 | 0.01 | 2.61 | 0.07 | 1.03 | 1.02 (1.06) | reference |
F1 | 0.02 | 1.21 | 2.71 | 1.01 | 3.15 | 2.13 | 3.11 | 0.01 | 0.75 | 1.64 | 0.13 | 1.44 (1.20) | 0.388 |
F2 | 0.04 | 3.04 | 2.92 | 1.43 | 2.55 | 1.09 | 3.14 | 3.09 | 2.88 | 0.97 | 2.85 | 2.18 (1.09) | 0.02 |
NC | 1.75 | 1.60 | 2.08 | 1.32 | 1.69 | 2.08 | 1.41 | 2.86 | 3.02 | 1.64 | 2.80 | 2.02 (0.61) | 0.015 |
7 Days | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | Mean (SD) | p-Value | |
BG0 | 0.62 | 0.39 | 0.62 | 0.48 | 0.83 | 0.58 | 0.62 | 0.22 | 0.16 | 0.28 | 0.18 | 0.45 (0.22) | reference |
BG1 | 0.69 | 0.57 | 0.56 | 1.06 | 0.94 | 0.62 | 0.52 | 0.28 | 0.28 | 0.23 | 0.25 | 0.55 (0.28) | 0.398 |
BG2 | 1.01 | 1.07 | 1.17 | 1.15 | 1.14 | 0.96 | 1.29 | 0.38 | 0.49 | 0.39 | 0.57 | 0.87 (0.34) | <0.01 |
NC | 1.24 | 1.14 | 1.74 | 1.65 | 1.58 | 0.94 | 1.20 | 0.97 | 1.04 | 1.20 | 1.37 | 1.28 (0.28) | <0.001 |
28 Days | |||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | Mean (SD) | p-Value | |
BG0 | 2.62 | 2.09 | 1.89 | 0.78 | 2.31 | 1.35 | 2.80 | 1.11 | 2.17 | 0.40 | 1.10 | 1.69 (0.79) | reference |
BG1 | 2.78 | 1.87 | 2.35 | 1.03 | 2.97 | 1.69 | 3.06 | 1.88 | 1.75 | 0.68 | 0.80 | 1.89 (0.84) | 0.563 |
BG2 | 2.93 | 2.95 | 2.81 | 0.83 | 2.40 | 2.75 | 2.96 | 2.16 | 2.18 | 1.01 | 1.18 | 2.20 (0.82) | 0.157 |
NC | 2.70 | 1.36 | 1.76 | 0.97 | 2.85 | 1.78 | 2.04 | 2.89 | 2.41 | 1.49 | 2.21 | 2.04 (0.63) | 0.267 |
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Aka, A.; Matsuura, T.; Yoshimura, A. An Evaluation of the Cytocompatibility of Endodontic Bioceramics in Human Periodontal-Ligament-Derived Cells. J. Funct. Biomater. 2024, 15, 231. https://doi.org/10.3390/jfb15080231
Aka A, Matsuura T, Yoshimura A. An Evaluation of the Cytocompatibility of Endodontic Bioceramics in Human Periodontal-Ligament-Derived Cells. Journal of Functional Biomaterials. 2024; 15(8):231. https://doi.org/10.3390/jfb15080231
Chicago/Turabian StyleAka, Asuka, Takashi Matsuura, and Atsutoshi Yoshimura. 2024. "An Evaluation of the Cytocompatibility of Endodontic Bioceramics in Human Periodontal-Ligament-Derived Cells" Journal of Functional Biomaterials 15, no. 8: 231. https://doi.org/10.3390/jfb15080231