The Effect of Mesoporous Bioactive Glass Nanoparticles/Graphene Oxide Composites on the Differentiation and Mineralization of Human Dental Pulp Stem Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis of MBN
2.2. Preparation and Characterization of the MBN/GO Composite
2.3. MBN/GO Composite Coating
2.4. Cell Culture
2.5. Cell Viability Assay
2.6. Alkaline Phosphatase (ALP) Activity Assay
2.7. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
2.8. Western Blot
2.9. Alizarin Red S Staining and Quantitative Analysis
2.10. Analysis of Wnt/β-Catenin Signaling Pathway-Related GENE expression in hDPSCs Cultured on MBN/GO Composites
2.11. Statistical Analysis
3. Results
3.1. Characterization of the MBN/GO Composite
3.1.1. FESEM
3.1.2. XRD
3.1.3. FTIR
3.1.4. Raman Spectroscopy
3.2. Viability of hDPSCs
3.3. ALP Activity in hDPSCs
3.4. qRT-PCR
3.5. Western Blot
3.6. Alizarin red S Staining and Analysis
3.7. Wnt/β-Catenin Signaling Pathway-Related Gene Expression in hDPSCs Cultured on MBN/GO Composites
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Powder’s Concentration % (w/v) | Samples | |
---|---|---|
0.05% | 0.01% | |
Surface coating concentration at culture plates (mg·cm–2) | 0.15625 mg·cm–2 | 0.03125 mg·cm–2 |
Gene | Primer | Sequence (5’-3’) | Size (bp) |
---|---|---|---|
β-actin | Forward | GCACTCTTCCAGCCTTCCTT | 150 |
Reverse | AATGCCAGGGTACATGGTGG | ||
DMP-1 | Forward | GGAGAGACAGCAAGGGTGAC | 87 |
Reverse | CACTGCTGGGACCATCTACG | ||
DSPP | Forward | GCTGGCCTGGATAATTCCGA | 135 |
Reverse | CTCCTGGCCCTTGCTGTTAT | ||
ALP | Forward | AATGTGGACACAGTGGCTGGA | 78 |
Reverse | TCTCCTGCTCAGTCATCTGCT | ||
BMP-2 | Forward | AAGCCAAACACAAACAGCGG | 104 |
Reverse | GGGAGCCACAATCCAGTCAT | ||
RUNX-2 | Forward | TCTGGCCTTCCACTCTCAGTA | 134 |
Reverse | TGGATAGTGCATTCGTGGGT | ||
MEPE | Forward | GCAGCTATCCACACCAGAAAG | 113 |
Reverse | GTTGAAATGTTGGTGCTGCC | ||
AXIN-2 | Forward | CCCTGCTGACTTGAGAGAGAC | 82 |
Reverse | CCCACTGAGTCTGGAATCTC | ||
β-catenin | Forward | CAGCGTGGACAATGGCTACT | 101 |
Reverse | AGATTCCTGCTGGTGGCTTG |
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Ahn, J.H.; Kim, I.-R.; Kim, Y.; Kim, D.-H.; Park, S.-B.; Park, B.-S.; Bae, M.-K.; Kim, Y.-I. The Effect of Mesoporous Bioactive Glass Nanoparticles/Graphene Oxide Composites on the Differentiation and Mineralization of Human Dental Pulp Stem Cells. Nanomaterials 2020, 10, 620. https://doi.org/10.3390/nano10040620
Ahn JH, Kim I-R, Kim Y, Kim D-H, Park S-B, Park B-S, Bae M-K, Kim Y-I. The Effect of Mesoporous Bioactive Glass Nanoparticles/Graphene Oxide Composites on the Differentiation and Mineralization of Human Dental Pulp Stem Cells. Nanomaterials. 2020; 10(4):620. https://doi.org/10.3390/nano10040620
Chicago/Turabian StyleAhn, Jae Hwa, In-Ryoung Kim, Yeon Kim, Dong-Hyun Kim, Soo-Byung Park, Bong-Soo Park, Moon-Kyoung Bae, and Yong-Il Kim. 2020. "The Effect of Mesoporous Bioactive Glass Nanoparticles/Graphene Oxide Composites on the Differentiation and Mineralization of Human Dental Pulp Stem Cells" Nanomaterials 10, no. 4: 620. https://doi.org/10.3390/nano10040620