Green Synthesis and Characterization of Novel Silver Nanoparticles Using Achillea maritima subsp. maritima Aqueous Extract: Antioxidant and Antidiabetic Potential and Effect on Virulence Mechanisms of Bacterial and Fungal Pathogens
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Plant Extract and Biosynthesis of Silver Nanoparticles
2.2. Characterization of Silver Nanoparticles
2.3. Experimental Setup of Fluorescence Analysis
2.4. Antioxidant Activity
2.4.1. DPPH Radical Scavenging Activity
2.4.2. Total Antioxidant Activity (TAA)
2.5. Antidiabetic Effect
2.5.1. α Amylase Inhibitory Assay
2.5.2. α Glucosidase Inhibitory Assay
2.6. Cytotoxic Effect on Human Cells
2.7. Antimicrobial Potential
2.7.1. Agar Well Diffusion Method
2.7.2. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration
2.7.3. In Vitro Silver Nanoparticles’ Effect on Lipopolysaccharides and Bacterial Biofilm
2.7.4. Silver Nanoparticles’ Effect on DNA Bacterial Genome
2.8. Lysozyme Activity
2.9. Silver Nanoparticles’ Effect on Candida Species Growth and Virulence
2.10. Statistical Analysis
3. Results
3.1. Characterization of Silver Nanoparticles
3.1.1. UV-Visible Spectroscopic Analysis
3.1.2. Fluorescence Analysis
3.1.3. FTIR Analysis
3.1.4. Structural Properties
3.2. Antioxidant Activity
3.3. Antidiabetic Effect
3.4. Cytotoxic Effect of Silver Nanoparticles
3.5. Antimicrobial Potential
3.5.1. Agar Well Diffusion Method
3.5.2. MIC, MBC and MFC Determinations
3.5.3. Silver Nanoparticles’ Effect on Bacterial Mechanism of Action
3.6. Silver Nanoparticles’ Effect on Candida albicans Growth and Virulence Factor
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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DPPH Free Radical Scavenging Activity IC50 (µg/mL) | |
AgNPs | 89.46 ± 2.48 a |
Ascorbic acid | 22.54 ± 0 b |
Total Antioxidant Activity (mg AAE/g DM) | |
AgNPs | 1.88 ± 0.01 a |
Ascorbic acid | 0.80 ± 0.01 b |
Clinical Pathogens | AgNPs | Standards | |
---|---|---|---|
Bacteria | Ceftazidime CAZ30 | Tobramycin | |
Escherchia coli | 15.8 b ± 0 | 15 e ± 0 | 25 a ± 0 |
Salmonella typhi | 14.5 c ± 0 | 26 b ± 0.5 | 18 d ± 0.5 |
Pseudomonas aeruginosa | 12.5 d | 17 d ± 0 | 22 c ± 0.5 |
Staphylococcus aureus | 0 e | 18 c ± 0 | 14.5 e ± 0 |
Yeasts | Fluconazole 25 | Amphotericin B | |
Candida albicans | 16.5 a | 13.5 f ± 0 | 23 b ± 0 |
Candida tropicalis | 0 e | 35 a ± 0.5 | 22 c ± 0 |
Bacterial Strains | MIC | MBC | Ratio MBC/MIC |
---|---|---|---|
Escherchia coli | 6.75 b | 12.5 b | 2 b |
Pseudomonas aeruginosa | 6.75 b | 12.5 b | 2 b |
Salmonella typhi | 6.75 b | 25 a | 4 a |
Fungal Strains | MIC | MFC | Ratio MFC/MIC |
Candida albicans | 12.5 a | 25 a | 2 b |
Bacterial Species | Biofilm Eradication (I%) | Lipopolysaccharide Degradation (I%) | DNA Damage |
---|---|---|---|
Pseudomonas aeruginosa | 79 | 47 | + |
Escherchia coli | 62 | 65 | + |
Salmonella typhi | 82 | 58 | + |
Culture Conditions of C. albicans | Cell Growth DO600 nm, 48 h (%) | Biofilm Formation and Morphogenesis | Hydrolytic Enzyme Production (Pz in mm) ±SD | |
---|---|---|---|---|
Lipase Pz | Proteinase Pz | |||
Untreated C. albicans | 0.174 ± 0.04 | +++ Morphogenesis change: germ tube; chlamydospores; pseudofilament | Pz = 0.6 ± 0 | Pz = 0.54 ± 0.1 |
C. albicans treated with AgNPs at 50 µg/mL | 0.166 ± 0.03 | (-) Altered blastospores (1), abnormal germinative tube (2), agglomeration (3) | Pz = 1 negative | Pz = 1 negative |
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Essghaier, B.; Hannachi, H.; Nouir, R.; Mottola, F.; Rocco, L. Green Synthesis and Characterization of Novel Silver Nanoparticles Using Achillea maritima subsp. maritima Aqueous Extract: Antioxidant and Antidiabetic Potential and Effect on Virulence Mechanisms of Bacterial and Fungal Pathogens. Nanomaterials 2023, 13, 1964. https://doi.org/10.3390/nano13131964
Essghaier B, Hannachi H, Nouir R, Mottola F, Rocco L. Green Synthesis and Characterization of Novel Silver Nanoparticles Using Achillea maritima subsp. maritima Aqueous Extract: Antioxidant and Antidiabetic Potential and Effect on Virulence Mechanisms of Bacterial and Fungal Pathogens. Nanomaterials. 2023; 13(13):1964. https://doi.org/10.3390/nano13131964
Chicago/Turabian StyleEssghaier, Badiaa, Hédia Hannachi, Rihem Nouir, Filomena Mottola, and Lucia Rocco. 2023. "Green Synthesis and Characterization of Novel Silver Nanoparticles Using Achillea maritima subsp. maritima Aqueous Extract: Antioxidant and Antidiabetic Potential and Effect on Virulence Mechanisms of Bacterial and Fungal Pathogens" Nanomaterials 13, no. 13: 1964. https://doi.org/10.3390/nano13131964