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Article

Detection of Tobacco Bacterial Wilt Caused by Ralstonia solanacearum by Combining Polymerase Chain Reaction with an α-Hemolysin Nanopore

by
Ying Wang
1,†,
Yusen Li
1,†,
Xin Zhou
1,
Wenna Zhang
2,
Shusheng Zhang
1 and
Dongmei Xi
1,*
1
Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Life Science, Linyi University, Linyi 276005, China
2
Shandong (Linyi) Institute of Modern Agriculture, Zhejiang University, Linyi 276000, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Nanomaterials 2023, 13(2), 332; https://doi.org/10.3390/nano13020332
Submission received: 14 November 2022 / Revised: 10 January 2023 / Accepted: 11 January 2023 / Published: 13 January 2023
(This article belongs to the Special Issue Nanomaterials for Sensors, Actuators and Environmental Applications)
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Abstract

:
Tobacco bacterial wilt is a serious disease caused by the soil-borne bacterium Ralstonia solanacearum (R. solanacearum). Herein, a rapid and purification-free α-hemolysin (α-HL) nanopore-sensing strategy based on polymerase chain reaction (PCR) and lambda exonuclease digestion was established to detect R. solanacearum. A 198-nucleotide-long single-stranded DNA was obtained via asymmetric PCR or the lambda exonuclease-mediated digestion of the PCR product. The DNA fragment produced unique long-lived, current-blocking signals when it passed through the α-HL nanopore. This sensing approach can allow for the determination of R. solanacearum in tobacco samples and can be conveniently extended to other DNA monitoring because of the extremely wide range of PCR applications.

1. Introduction

The rapid clinical or agricultural detection of pathogenic DNA usually requires polymerase chain reaction (PCR) amplification [1] to obtain double-stranded DNA (dsDNA) products, which are then identified using fluorescence-based [2] or lateral flow strip methods [3]. Generally, the fluorescence-based methods require applying dyes or modified luminescent and quenched groups to the probe and corresponding instruments [4,5]. Reaction conditions, such as temperature, solvent, acidity, and fluorescence quenchers, affect the intensity of fluorescent signals [6]. The lateral flow strip method is simple and rapid; however, it suffers from shortcomings, such as low throughput and high cost, and its accuracy is directly related to the specificity of the capture agent [7,8]. Therefore, the development of an alternative rapid and label-free detection method with good accuracy is necessary [9].
Nanopore has emerged as an attractive single-molecule tool that offers rapid, low-cost, and label-free analysis [10]. The ionic current fluctuation through the nanopore caused by an analyte binding can be utilized to reveal the identity and concentration of the analyte [11]. To date, the nanopore technique has been widely developed for the detection of various analytes including DNA [12], microRNA [13], peptides [14,15], small molecules [16], metal ions [17], and biomacromolecules [18,19]. Nanopore sequencing evolved from α-HL biological nanopores to MinION solid-state nanopores developed by the Oxford Nanopore Corporation and have been used to analyze human, cancer, pathogen, and other samples [20,21,22]. Considering that PCR is widely used to amplify DNA, the combination of PCR and a nanopore sensor could greatly expand the application range of nanopore technology. Recently, SiNx solid-state nanopores with a diameter >5 nm have been used as downstream sensors for PCR products, and clinical samples of group A Streptococcus have been successfully identified [23]. However, it remains challenging to distinguish the blocking current signals of PCR products from those generated by the molecules in PCR buffer.
Tobacco bacterial wilt is a serious soil-borne disease caused by Ralstonia solanacearum (R. solanacearum) [24]. It blocks vascular bundles and causes wilting in plants until the entire plant dies [25]. In this study, we present a facile strategy for monitoring the DNA of R. solanacearum by coupling PCR with a nanopore sensor. In general, the size of the channel diameter is the dominant factor that affects the distinguishing ability and application scope of the nanopore. Herein, α-hemolysin (α-HL), a well-studied bacterial-derived toxin with the narrowest constriction of 1.4 nm [26,27,28,29], was utilized for analysis. A single-stranded DNA (ssDNA) with 198 nucleotides was obtained via asymmetric PCR or the lambda exonuclease-mediated degradation of the PCR product. In the presence of a salt concentration gradient, the ssDNA generated remarkably long current-blocking signals with a high capture rate when passing through the α-HL nanopore. Notably, the unique long-lived signals were easily distinguished from those generated by materials in the PCR reaction buffer. This method was used to detect R. solanacearum in tobacco field samples without labeling or sample purification, and the results obtained were consistent with those obtained via sequencing. Due to the wide application of the PCR method, this strategy combining PCR and a nanopore sensor has great potential in nucleic acid monitoring applications.

2. Experimental Section

2.1. Chemicals

1,2-Diphytanoyl-sn-glycero-3-phosphocholine (chloroform, ≥99%) was purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA). α-HL (lyophilized powder from Staphylococcus aureus) and decane (anhydrous, ≥99%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trans DNA Marker I was purchased from Beijing Transgen Biotechnology Co., Ltd. (Beijing, China). Lambda exonuclease was purchased from ThermoFisher Scientific Ltd. (Shanghai, China). Rapid Taq Master Mix was purchased from Vazyme Biotech Co., Ltd. (Nanjing, China). A Rapid DNA Extraction and Detection Kit (KG203) and Universal DNA Purification kit (DP214) were purchased from Tiangen Biochemical Technology Co., Ltd. (Beijing, China). All other reagents were of analytical grade and used without further purification. One positive and five field-collected tobacco samples were kindly provided by the Laboratory of Plant Protection Research Center at the Tobacco Research Institute of the Chinese Academy of Agricultural Sciences. Cyto-1F (5’-TTCCAGTATCTCAGCCCGGA-3’), Cyto-1R (5’-GGTCACCTTGAAGACGCCAG-3’), and p-Cyto-1R (5’-pGGTCACCTTGAAGACGCCAG-3’) were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). The 198-nucleotides sequence (strand A) was synthesized and purified by Takara Bio Inc. (Dalian, China).

2.2. Apparatus

DNA concentration was determined using NanoDrop One (Thermo Scientific, Waltham, MA, USA). PCR was performed using a mini-amplifier (Thermo Scientific, Waltham, MA, USA). The electrophoresis apparatus DYY-8C (Liuyi, Beijing, China) and a horizontal electrophoresis cell, DYCP-31DN (Liuyi, Beijing, China), were used for gel electrophoresis. Gel images were captured using a Universal Hood II Imaging System (Bio-Rad, Hercules, CA, USA). Current was measured using a patch-clamp amplifier (Axon 200 B, AXON, Scottsdale, AZ, USA) and converted into data using a DigiData 1440A converter (Molecular Devices, San Jose, DE, USA).

2.3. DNA Extraction and PCR

The genomic DNA of R. solanacearum (1.5 mL bacterial culture) or the total DNA of tobacco samples infected with R. solanacearum (about 0.5 g) were extracted and purified using the rapid DNA extraction and detection kit. The genome was used as the template for PCR. The target sequences of R. solanacearum were amplified in a 20 μL reaction volume containing 10 μL 2× Rapid Taq Master Mix, 1 μL Cyto-1F (10 μM), 1 μL p-Cyto-1R (10 μM) (or 0.25 μL Cyto-1R (10 μM) in asymmetric PCR), 1 μL genomic DNA, and 7 μL H2O [30]. Thermal cycling consisted of denaturation at 94 °C for 1 min, 30 cycles at 94 °C for 30 s, 60 °C for 30 s, and 72 °C for 30 s, and a final extension at 72 °C for 5 min. The PCR products were separated using a 2% agarose gel stained with GelRed dye and visualized using the Universal Hood II Imaging System. The positive PCR products of the tobacco samples were submitted to Sangon Biotech Co., Ltd. (Shanghai, China) for sequencing.

2.4. Digestion by Lambda Exonuclease

In the presence of lambda exonuclease, the PCR product was degraded stepwise from its 5-phosphorylated end into ssDNA. The reaction was carried out using 50 µL 1× reaction buffer (67 mM glycine-KOH, 2.5 mM MgCl2 0.01% (v/v), Triton X-100 (pH 9.4 at 25 °C)). The PCR product (10 μL) and lambda exonuclease (1 U) were then added to the reaction solution, and the reaction was performed at 25 °C for 5 min, followed by incubation at 85 °C for 5 min to inactivate lambda exonuclease.

2.5. Nanopore Electrical Recording and Data Analysis

A lipid bilayer membrane was formed by spanning a 150 μm orifice in a Delrin bilayer cup that was partitioned into two chambers: cis and trans. Both chambers were filled with 1 mL of buffer (1 M KCl, 1 mM EDTA, and 25 mM HEPES; pH 7.0). α-HL was injected close to the hole in the cis chamber, and insertion was confirmed by a definite jump in the current values. When a stable single-pore insertion was detected, DNA was added to the cis chamber immediately adjacent to the aperture. A positive potential of 150 mV was applied to the trans chamber of the detection cell using an Ag/AgCl electrode. The conditions for nanopore electrical recording using gradient salt concentrations were similar to those of equal salt concentrations; however, the concentration and voltage were changed. Both chambers were filled with 1 mL of buffer (cis: 0.75 M KCl, 1 mM EDTA, 25 mM HEPES, pH 7.0; trans: 3 M KCl, 1 mM EDTA, 25 mM HEPES, pH 7.0). A positive potential of 100 mV was applied to the trans chamber of the detection cell using an Ag/AgCl electrode. Nanopores were measured at 25 ± 2 °C. After a nanopore electrical recording test, the ssDNA in the two chambers were extracted and purified using the Universal DNA Purification kit and amplified with the PCR method according to Section 2.3 of the paper.
Current was measured using the patch-clamp amplifier and converted into data using a DigiData 1440A converter. The signals were filtered at 5 kHz and acquired at a sampling rate of 100 kHz using PClamp 10.6 (Axon Instruments, Foster City, CA, USA). Finally, data were analyzed using MATLAB (R2013b, MathWorks, Natick, MA, USA), OriginLab 9.0 (OriginLab Corporation, Northampton, MA, USA), and software programmed by Long’s group [31]. The original data of the Axon Binary Data files (ABF) of the nanopore experiment were converted into TXT files using Cutting software programmed by Long’s group [31]. The TXT files were processed using MATLAB, and the current signals through the nanopore were screened. The screened data were imported into OriginLab 9.0 for function fitting, and scatter plots and blocking degrees were obtained. Values in the error bars were calculated using the “Statistics” function of OriginLab 9.0.

3. Results

3.1. Feasibility of Detecting R. solanacearum by Combining Asymmetric PCR with an α-HL Nanopore

This strategy was used to detect R. solanacearum by combining asymmetric PCR with nanopore sensing. As shown in Scheme 1, genomic DNA was extracted from the R. solanacearum cells. The mixture of single- and double-stranded DNA was obtained via asymmetric PCR using the genomic DNA of R. solanacearum as the template and the primer pair Cyto-1F/Cyto-1R. The nanopore formed by α-HL was used as the sensor element. The diameter of ssDNA is smaller than the inner diameter of α-HL, and the diameter of dsDNA is larger than the inner diameter of α-HL. Therefore, ssDNA can pass through the α-HL nanopore in the electric field, causing characteristic current blocks. The passage of dsDNA through the α-HL nanopore is difficult without a specific guide strand at its end [32,33].
The PCR product contains dsDNA, and the two single strands are called strands A and B. Here, strand A (red in Scheme 1) was produced via PCR amplification using Cyto-1F as a primer and its complementary strand, B, as a template. Similarly, strand B (green in Scheme 1) was generated using Cyto-1R as a primer and complementary strand A as a template. To verify whether asymmetric PCR products were generated, we first monitored amplicons using 2% agarose gel electrophoresis. As shown in Figure 1A, the primer pair Cyto-1F/Cyto-1R was used to amplify the genomic DNA of R. solanacearum at a volume ratio of 4:1, and a mixture of strand A (198 nucleotides) and dsDNA (198 base pairs) was obtained (Lane 3). In addition, a negative control was obtained via PCR amplification using ultrapure water as a template (Lane 4).
Next, in the presence of a positive potential of 150 mV, a nanopore-based assay was employed to analyze asymmetric PCR products in 1 M KCl (cis/trans). In the negative control experiment, several block events appeared in the current trace (Figure 1B, top). Scatter plots showed that these blocking events had blocking degrees within a range of 0.2–0.96 (Figure 1C), similar to the results reported by King’s group [23]. The PCR mixture contained replicase, bovine serum albumin, and other molecules, and these molecules produced translocation signals in the nanopore assay. Some of these signals also may be caused by collisions.
In the presence of asymmetric PCR products with both single-stranded and double-stranded DNA, we observed several markedly prolonged events (Figure 1B, bottom) that were easily discriminated from those generated by the negative control. For statistical analysis, I0 and I were designated as the open pore current and blockage current, respectively. The scatter plot showing current blockage and duration yielded two populations upon the addition of asymmetric PCR products (Figure 1D), of which P2 had characteristics similar to those of the negative control group (Figure 1C). Notably, the newly emerged population, P1, had a wide distribution of dwell time, from 22.49 to 2067.07 ms. These signals are clearly different from those generated by the molecules in the PCR mixture, initially confirming the translocation of the 198-nucleotide-long DNA strand through the nanopore.

3.2. Enhancing the Performance of the PCR–α-HL Strategy Using Lambda Exonuclease and Gradient Salt Concentration

Although the presence of R. solanacearum could be detected by combining asymmetric PCR with a nanopore, the capture rate of the ssDNA was rather low, and the α-HL nanopore was frequently blocked, sometimes reversibly and, more often, irreversibly, probably because the dsDNA with blunt ends could not pass through the nanopore and blocked it.
To address those issues, we aimed to obtain only one strand of the PCR product via enzymatic digestion (Scheme 2). The genomic DNA of R. solanacearum was amplified via PCR using the primers Cyto-1F/p-Cyto-1R. The 5’-terminal hydroxyl group of the primer p-Cyto-1R was linked to a phosphate group, so the PCR product contained a phosphate group at the 5’-terminus of strand B, and no phosphate group was present at the 5’-end of strand A. Lambda exonuclease is a 5’-3’ exonuclease that catalyzes the stepwise removal of nucleotides at the 5’-phosphorylated strands of dsDNA, with low activity for non-phosphorylated substrates. Therefore, lambda exonuclease degraded strand B, whereas strand A remained unaffected. It passed through the α-HL nanopore and produced characteristic current blocks.
As shown in Figure 2A, the genomic DNA of R. solanacearum was amplified using the primer pair Cyto-1F/p-Cyto-1R, and a bright band was observed on a 2% agarose gel (lane 2). Lambda exonuclease degraded strand B of the dsDNA, and ssDNA (strand A) was produced. The brightness of the band corresponding to strand A was significantly lower than that of the PCR products (Lane 3).
Asymmetric salt concentration across the α-HL nanopore can significantly improve the capture rate of target DNA [34]. We speculated that this strategy could also be effective in this assay. As expected, the unpurified lambda exonuclease-digested PCR products containing strand A in asymmetric KCl solutions (0.75 M/3 M, cis/trans) across the nanopore produced significantly more current blocks than those in 1 M KCl (cis/trans) conditions (Figure 2B). In addition, the lambda exonuclease-treated PCR product (strand A) was purified by the Universal DNA Purification kit. Strand A was synthesized and purified by Takara Bio Inc. The duration of both kinds of strand A was decreased as the voltage increased. At the end of the translocation test, the synthesized strand A in the two chambers were purified by the Universal DNA Purification kit, which was used as the template for the PCR. The results show that strand A was amplified by the trans chamber and the cis chamber. These experimental results verified that strand A was translocated to the trans chamber (Figures S1–S7).
Importantly, because of the improved capture rate, this strategy recognized the ssDNA of R. solanacearum more efficiently. In contrast to the scatter plot of the negative control experiment (Figure 3A,C), a new cluster of multiple signals appeared in the scatter diagram of the target DNA (Figure 3D). In this population, the current blockage (I/I0) was greater than that of the negative control. Statistical analysis showed that the I/I0 of the higher population was fitted to the Gauss distribution with a value of 0.79 ± 0.0057 (Figure 3E). The duration lay within a range of 20.11–7232.85 ms and statistically fitted the exponential function with a value of 165.50 ± 16.15 ms (Figure 3F). These data, integrated with the results of electrophoresis, suggested that the population was generated by the translocation of strand A through the nanopore and can serve as output DNA signatures for R. solanacearum analysis. The results indicate that the performance of the PCR-α-HL strategy was effectively improved using lambda exonuclease and gradient salt concentration.

3.3. Application of This Method to Tobacco Fields

PCR and DNA sequencing are the most common methods used to identify pathogens. To verify the applicability of this strategy in field samples, one positive and five field samples were tested using this approach and DNA sequencing (Figures S8–S14). The results of both methods indicated that the positive sample and four field samples were infected with R. solanacearum. The detection efficiency of the two methods is consistent, indicating that the PCR–α-HL nanopore strategy can be used to detect R. solanacearum in tobacco fields (Table 1).

4. Conclusions

In this study, we established a rapid, purification-free, and label-free strategy for detecting R. solanacearum by combining PCR with an α-HL nanopore. Current blocking produced by the translocation of ssDNA was clearly distinguishable from that generated by molecules in the PCR mixture. To the best of our knowledge, this is the first report of the PCR–α-HL-nanopore-based detection of R. solanacearum and its tobacco field samples, which lays a solid foundation for establishing an early warning mechanism for tobacco bacterial wilt. In the future, the coupling of isothermal amplification methods with nanopore sensors will be further studied, and nanopore-based methods will be used for the multiple detection of pathogens in certain samples.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/nano13020332/s1. Figure S1. 2% Agarose gel electrophoresis of the PCR product. Lane 1, DNA Ladder L(100~3000 bp). Lane 2, PCR product of pure water (negative control). PCR product of solution in cis chamber (lane 3) and trans chamber (lane 4). Strand A in the two chambers were purified by the Universal DNA Purification kit, which was used as the template for PCR. Figure S2. Durations of the synthetic strand A were decreased as the voltage increased from 100 to 150 mV. Figure S3. I/I0 histogram of events of the strand A from +100 mV to +150 mV in 0.75 M/3 M (cis/trans) KCl. Figure S4. Duration histogram of events generated by strand A from +100 mV to +150 mV in 0.75 M/3 M (cis/trans) KCl. Figure S5. Durations of the Universal DNA Purification kit-purified strand A were decreased as the voltage increased from 100 to 130 mV. Figure S6. I/I0 histogram of events generated by the Universal DNA Purification kit-purified strand A under gradient voltage from +100 mV to +130 mV in 0.75 M/3 M (cis/trans) KCl. Figure S7. Duration histogram of events generated by the Universal DNA Purification kit-purified strand A under voltage from +100 mV to +130 mV in 0.75 M/3 M (cis/trans) KCl. Figure S8. 2% Agarose gel electrophoresis of the PCR product. Lane 1, Trans DNA Marker I. Lane 2, PCR product of R. solanacearum genome. Lane 3–7, PCR product of field tabacco samples of 1–5. Figure S9. Current trace of the 4 positive field samples. All nanopore data were recorded at +100 mV in 0.75 M/3 M (cis/trans) KCl, and the same below. Figure S10. Scatter plot of the 4 positive field samples. Figure S11. I/I0 histogram of the events produced by the 4 positive field samples. Figure S12. DNA sequencing of the 4 positive field sample. Alignment of DNA sequencing results of the 4 positive field samples by Basic Local Alignment Search Tool in NCBI website. Figure S13. Current trace of the negative field sample (sample 5). Figure S14. Scatter plot of the sample 5 (n = 524).

Author Contributions

Conceptualization and methodology, D.X. and Y.W.; software, W.Z.; validation, Y.L. and X.Z.; resources, Y.L. and X.Z.; data curation, Y.L. and X.Z.; writing—original draft preparation, Y.W.; writing—review and editing, Y.W. and D.X.; supervision, D.X. and Y.W; project administration, D.X. and S.Z.; funding acquisition, D.X., Y.W. and S.Z. All authors have read and agreed to the published version of the manuscript.

Funding

The National Natural Science Foundation of China (22276085, 21874062, 22076073); the Open Project Foundation of the Shandong (Linyi) Institute of Modern Agriculture, Zhejiang University (ZDNY-2021-FWLY02012); the Key Research and Development Project of Linyi City (2021030); and commissioned research projects of Linyi University (HX210035, HX210046).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available in the main manuscript and in the Supplementary Materials.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Mullis, K.B.; Saiki, R.K.; Scharf, S.; Faloona, F.; Horn, G.T.; Erlich, H.A.; Arnheim, N. Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 1985, 230, 1350–1354. [Google Scholar]
  2. Wang, Y.; Li, B.; Zhang, N.; Xi, D.; Liu, J.; Zhou, H. Viral cdna-based extension for highly sensitive fluorescence detection of DNA methyltransferase activity. Sens. Actuators B Chem. 2018, 255, 3488–3494. [Google Scholar] [CrossRef]
  3. Wu, Y.D.; Xu, M.J.; Wang, Q.Q.; Zhou, C.X.; Wang, M.; Zhu, X.Q.; Zhou, D.H. Recombinase polymerase amplification (rpa) combined with lateral flow (lf) strip for detection of toxoplasma gondii in the environment. Vet. Parasitol. 2017, 243, 199–203. [Google Scholar] [CrossRef] [PubMed]
  4. Huang, Y.; Chen, W.; Chung, J.; Yin, J.; Yoon, J. Recent progress in fluorescent probes for bacteria. Chem. Soc. Rev. 2021, 50, 7725–7744. [Google Scholar] [CrossRef]
  5. Rabber, L.; Ernest, T.E.; Gelkop, Y.M.; Lahav, M.C.; Douvdevani, A.; Nahum, A. Fast fluorometric method for measuring circulating cell-free DNA could aid the diagnosis of febrile children. Acta Paediatr. 2021, 110, 1577–1578. [Google Scholar] [CrossRef]
  6. Chen, B.-B.; Liu, M.-L.; Gao, Y.-T.; Chang, S.; Qian, R.-C.; Li, D.-W. Design and applications of carbon dots-based ratiometric fluorescent probes: A review. Nano Res. 2022, 16, 1064–1083. [Google Scholar] [CrossRef]
  7. Wang, C.; Chen, X.; Wu, Y.; Li, H.; Wang, Y.; Pan, X.; Tang, T.; Liu, Z.; Li, X. Lateral flow strip for visual detection of k-ras mutations based on allele-specific pcr. Biotechnol. Lett. 2016, 38, 1709–1714. [Google Scholar] [CrossRef]
  8. Gong, X.; Cai, J.; Zhang, B.; Zhao, Q.; Piao, J.; Peng, W.; Gao, W.; Zhou, D.; Zhao, M.; Chang, J. A review for fluorescent lateral flow immunochromatographic strip. J. Mater. Chem. B 2017, 5, 5079–5091. [Google Scholar] [CrossRef]
  9. Laszlo, A.H.; Derrington, I.M.; Ross, B.C.; Brinkerhoff, H.; Adey, A.; Nova, I.C.; Craig, J.M.; Langford, K.W.; Samson, J.M.; Daza, R.; et al. Decoding long nanopore sequencing reads of natural DNA. Nat. Biotechnol. 2014, 32, 829–833. [Google Scholar] [CrossRef] [Green Version]
  10. Wang, Y.; Zhang, Y.; Chen, X.; Guan, X.; Wang, L. Analysis with biological nanopore: On-pore, off-pore strategies and application in biological fluids. Talanta 2021, 223, 121684. [Google Scholar] [CrossRef]
  11. Tang, W.; Fried, J.P.; Tilley, R.D.; Gooding, J.J. Understanding and modelling the magnitude of the change in current of nanopore sensors. Chem. Soc. Rev. 2022, 51, 5757–5776. [Google Scholar] [CrossRef] [PubMed]
  12. Wen, S.; Zeng, T.; Liu, L.; Zhao, K.; Zhao, Y.; Liu, X.; Wu, H.C. Highly sensitive and selective DNA-based detection of mercury(ii) with α-hemolysin nanopore. J. Am. Chem. Soc. 2011, 133, 18312–18317. [Google Scholar] [CrossRef] [PubMed]
  13. Wanunu, M.; Dadosh, T.; Ray, V.; Jin, J.; Mcreynolds, L.; Drndic, M. Rapid electronic detection of probe-specific micrornas using thin nanopore sensors. Nat. Nanotechnol. 2010, 5, 807–814. [Google Scholar] [CrossRef] [PubMed]
  14. Huo, M.Z.; Li, M.Y.; Ying, Y.L.; Long, Y.T. Is the volume exclusion model practicable for nanopore protein sequencing? Anal. Chem. 2021, 93, 11364–11369. [Google Scholar] [CrossRef]
  15. Zhou, S.; Wang, H.; Chen, X.; Wang, Y.; Zhou, D.; Liang, L.; Wang, L.; Wang, D.; Guan, X. Single-molecule study on the interactions between cyclic nonribosomal peptides and protein nanopore. ACS Appl. Bio. Mater. 2020, 3, 554–560. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  16. Ramsay, W.J.; Bayley, H. Single-molecule determination of the isomers of d-glucose and d-fructose that bind to boronic acids. Angew. Chem. Int. Ed. Engl. 2018, 57, 2841–2845. [Google Scholar] [CrossRef]
  17. Orit, B.; Gu, L.-Q.; Zhou, L.; Lu, X.; Cheley, S.; Bayley, H. Simultaneous stochastic sensing of divalent metal ions. Nat. Biotechnol. 2000, 18, 1005–1007. [Google Scholar]
  18. Mayer, S.F.; Cao, C.; Dal Peraro, M. Biological nanopores for single-molecule sensing. IScience 2022, 25, 104145. [Google Scholar] [CrossRef]
  19. Zhou, S.; Wang, L.; Chen, X.; Guan, X. Label-free nanopore single-molecule measurement of trypsin activity. ACS Sens. 2016, 1, 607–613. [Google Scholar] [CrossRef] [Green Version]
  20. Kasianowicz, J.J.; Brandin, E.; Branton, D.; Deamer, D.W. Characterization of individual polynucleotide molecules using a membrane channel. Proc. Natl. Acad. Sci. USA 1996, 93, 13770–13773. [Google Scholar] [CrossRef] [Green Version]
  21. Ashkenasy, N.; Sanchez-Quesada, J.; Bayley, H.; Ghadiri, M.R. Recognizing a single base in an individual DNA strand: A step toward DNA sequencing in nanopores. Angew. Chem. Int. Ed. Engl. 2005, 44, 1401–1404. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  22. Wang, Y.; Zhao, Y.; Bollas, A.; Wang, Y.; Au, K.F. Nanopore sequencing technology, bioinformatics and applications. Nat. Biotechnol. 2021, 39, 1348–1365. [Google Scholar] [CrossRef] [PubMed]
  23. King, S.; Briggs, K.; Slinger, R.; Tabard-Cossa, V. Screening for group a streptococcal disease via solid-state nanopore detection of pcr amplicons. ACS Sens. 2022, 7, 207–214. [Google Scholar] [CrossRef]
  24. Genin, S. Molecular traits controlling host range and adaptation to plants in ralstonia solanacearum. N. Phytol. 2010, 187, 920–928. [Google Scholar] [CrossRef]
  25. Bittner, R.J.; Arellano, C.; Mila, A.L. Effect of temperature and resistance of tobacco cultivars to the progression of bacterial wilt, caused by ralstonia solanacearum. Plant Soil 2016, 408, 299–310. [Google Scholar] [CrossRef]
  26. Haugland, M.M.; Borsley, S.; Cairns-Gibson, D.F.; Elmi, A.; Cockroft, S.L. Synthetically diversified protein nanopores: Resolving click reaction mechanisms. ACS Nano 2019, 13, 4101–4110. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  27. Liu, Y.; Zhang, S.; Wang, Y.; Wang, L.; Cao, Z.; Sun, W.; Fan, P.; Zhang, P.; Chen, H.Y.; Huang, S. Nanopore identification of alditol epimers and their application in rapid analysis of alditol-containing drinks and healthcare products. J. Am. Chem. Soc. 2022, 144, 13717–13728. [Google Scholar] [CrossRef]
  28. Jeong, K.B.; Luo, K.; Lee, H.; Lim, M.C.; Yu, J.; Choi, S.J.; Kim, K.B.; Jeon, T.J.; Kim, Y.R. Alpha-hederin nanopore for single nucleotide discrimination. ACS Nano 2019, 13, 1719–1727. [Google Scholar] [CrossRef]
  29. Rotem, D.; Jayasinghe, L.; Salichou, M.; Bayley, H. Protein detection by nanopores equipped with aptamers. J. Am. Chem. Soc. 2012, 134, 2781–2787. [Google Scholar] [CrossRef]
  30. Hu, L.; Mu, W.; Guo, J.; Xue, C.; Feng, X.; Xi, J.; Zhang, Z.; Zhang, Y.; Li, Y.; Zhou, H.; et al. Locked nucleic acid-enhanced quantitative real-time pcr detection of ralstonia solanacearum in tobacco planting soil. Tob. Sci. Technol. 2017, 50, 14–21. [Google Scholar]
  31. Cao, C.; Long, Y.T. Biological nanopores: Confined spaces for electrochemical single-molecule analysis. Acc. Chem. Res. 2018, 51, 331–341. [Google Scholar] [CrossRef] [PubMed]
  32. Dragomir, I.S.; Bucataru, I.C.; Schiopu, I.; Luchian, T. Unzipping mechanism of free and polyarginine-conjugated DNA-pna duplexes, preconfined inside the α-hemolysin nanopore. Anal. Chem. 2020, 92, 7800–7807. [Google Scholar] [CrossRef] [PubMed]
  33. Tan, C.S.; Riedl, J.; Fleming, A.M.; Burrows, C.J.; White, H.S. Kinetics of t3-DNA ligase-catalyzed phosphodiester bond formation measured using the α-hemolysin nanopore. ACS Nano 2016, 10, 11127–11135. [Google Scholar] [CrossRef] [PubMed]
  34. Xi, D.; Li, Z.; Liu, L.; Ai, S.; Zhang, S. Ultrasensitive detection of cancer cells combining enzymatic signal amplification with an aerolysin nanopore. Anal. Chem. 2018, 90, 1029–1034. [Google Scholar] [CrossRef] [PubMed]
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Scheme 1. Schematic illustration of the detection of R. solanacearum, combining asymmetric PCR with a nanopore.
Scheme 1. Schematic illustration of the detection of R. solanacearum, combining asymmetric PCR with a nanopore.
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Figure 1. Detection of R. solanacearum combining asymmetric PCR with an α-HL nanopore. (A) Agarose gel electrophoresis analysis of asymmetric PCR amplification. Lane 1: Trans Marker I. Lane 2: PCR product of R. solanacearum genome. Lane 3 and lane 4: asymmetric PCR product of R. solanacearum genome and pure water (negative control). The volume ratio of primers Cyto-1-F and Cyto-1-R was 4:1. (B) Current trace of the negative control (top) and the asymmetric PCR products (bottom). (C) Scatter plot of negative control (orange, n = 106). (D) Scatter plot of asymmetric PCR products (navy blue, n = 1432). All nanopore data were measured at +150 mV in 1 M/1 M (cis/trans) KCl.
Figure 1. Detection of R. solanacearum combining asymmetric PCR with an α-HL nanopore. (A) Agarose gel electrophoresis analysis of asymmetric PCR amplification. Lane 1: Trans Marker I. Lane 2: PCR product of R. solanacearum genome. Lane 3 and lane 4: asymmetric PCR product of R. solanacearum genome and pure water (negative control). The volume ratio of primers Cyto-1-F and Cyto-1-R was 4:1. (B) Current trace of the negative control (top) and the asymmetric PCR products (bottom). (C) Scatter plot of negative control (orange, n = 106). (D) Scatter plot of asymmetric PCR products (navy blue, n = 1432). All nanopore data were measured at +150 mV in 1 M/1 M (cis/trans) KCl.
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Scheme 2. Schematic illustration of R. solanacearum detection combining the PCR–lambda exonuclease method with an α-HL nanopore.
Scheme 2. Schematic illustration of R. solanacearum detection combining the PCR–lambda exonuclease method with an α-HL nanopore.
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Figure 2. (A) Agarose gel electrophoresis of the PCR product digested with lambda exonuclease. Lane 1: Trans Marker I. Lane 2: PCR product of R. solanacearum genome. Lane 3: PCR product incubated with lambda exonuclease. Lane 4: PCR product of pure water (negative control). (B) The frequency of the current trace of the lambda exonuclease-digested PCR products under equal salt conditions (cis/trans, 1M/1M KCl, +150 mV, top) or under asymmetric salt conditions (cis/trans, 0.75 M/3 M KCl, +100 mV, bottom).
Figure 2. (A) Agarose gel electrophoresis of the PCR product digested with lambda exonuclease. Lane 1: Trans Marker I. Lane 2: PCR product of R. solanacearum genome. Lane 3: PCR product incubated with lambda exonuclease. Lane 4: PCR product of pure water (negative control). (B) The frequency of the current trace of the lambda exonuclease-digested PCR products under equal salt conditions (cis/trans, 1M/1M KCl, +150 mV, top) or under asymmetric salt conditions (cis/trans, 0.75 M/3 M KCl, +100 mV, bottom).
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Figure 3. Detection of the ssDNA of R. solanacearum in lambda exonuclease-treated PCR product with an α-HL nanopore. Current trace of lambda exonuclease-digested PCR products of pure water (negative control) (A) and R. solanacearum (B). (C) Scatter plot of negative control (orange, n = 1218). (D) Scatter plot of the lambda exonuclease-treated PCR product of R. solanacearum (navy blue, n = 2616). (E) I/I0 histogram of the events produced by strand A (n = 428). (F) Duration histogram of events generated by strand A. All nanopore data were measured at +100 mV in 0.75 M/3 M (cis/trans) KCl.
Figure 3. Detection of the ssDNA of R. solanacearum in lambda exonuclease-treated PCR product with an α-HL nanopore. Current trace of lambda exonuclease-digested PCR products of pure water (negative control) (A) and R. solanacearum (B). (C) Scatter plot of negative control (orange, n = 1218). (D) Scatter plot of the lambda exonuclease-treated PCR product of R. solanacearum (navy blue, n = 2616). (E) I/I0 histogram of the events produced by strand A (n = 428). (F) Duration histogram of events generated by strand A. All nanopore data were measured at +100 mV in 0.75 M/3 M (cis/trans) KCl.
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Table
Table 1. Real sample detection.
Table 1. Real sample detection.
SampleSequencingThis Method
PositiveNegativePositiveNegative
11010
54141
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Wang, Y.; Li, Y.; Zhou, X.; Zhang, W.; Zhang, S.; Xi, D. Detection of Tobacco Bacterial Wilt Caused by Ralstonia solanacearum by Combining Polymerase Chain Reaction with an α-Hemolysin Nanopore. Nanomaterials 2023, 13, 332. https://doi.org/10.3390/nano13020332

AMA Style

Wang Y, Li Y, Zhou X, Zhang W, Zhang S, Xi D. Detection of Tobacco Bacterial Wilt Caused by Ralstonia solanacearum by Combining Polymerase Chain Reaction with an α-Hemolysin Nanopore. Nanomaterials. 2023; 13(2):332. https://doi.org/10.3390/nano13020332

Chicago/Turabian Style

Wang, Ying, Yusen Li, Xin Zhou, Wenna Zhang, Shusheng Zhang, and Dongmei Xi. 2023. "Detection of Tobacco Bacterial Wilt Caused by Ralstonia solanacearum by Combining Polymerase Chain Reaction with an α-Hemolysin Nanopore" Nanomaterials 13, no. 2: 332. https://doi.org/10.3390/nano13020332

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