A Curcumin-Decorated Nanozyme with ROS Scavenging and Anti-Inflammatory Properties for Neuroprotection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Chemicals
2.2. Synthesis of PM and PMC NPs
2.3. Characterization
2.4. Enzyme-Mimicking ROS Scavenging Ability
2.4.1. Total ROS Scavenging Ability
2.4.2. Enzyme-Mimicking Activity Measurements
2.5. Cytotoxicity Test and Hemolysis Assay
2.6. Fluorescence Assay of ROS Level
2.7. Immunofluorescence
2.8. Western Blot
2.9. Statistical Analysis
3. Results and Discussion
3.1. Synthesis and Characterization of PM and PMC NPs
3.2. ROS Scavenging Ability of PMC NPs Intracellular
3.3. Cell Compatibility and Hemocompatibility
3.4. PMC NPs Induces an Anti-Inflammatory Response in Microglia
3.5. PMC NPs Induce an Antioxidative Response and Cytoprotection
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Nathan, C.; Cunningham-Bussel, A. Beyond oxidative stress: An immunologist’s guide to reactive oxygen species. Nat. Rev. Immunol. 2013, 5, 349–361. [Google Scholar] [CrossRef] [PubMed]
- Sies, H.; Jones, D.P. Reactive oxygen species (ROS) as pleiotropic physiological signalling agents. Nat. Rev. Mol. Cell Biol. 2020, 7, 363–383. [Google Scholar] [CrossRef] [PubMed]
- Oberkampf, M.; Guillerey, C.; Mouriès, J.; Rosenbaum, P.; Fayolle, C.; Bobard, A.; Savina, A.; Ogier-Denis, E.; Enninga, J.; Amigorena, S.; et al. Mitochondrial reactive oxygen species regulate the induction of CD8+ T cells by plasmacytoid dendritic cells. Nat. Commun. 2018, 1, 2241. [Google Scholar] [CrossRef] [PubMed]
- Sies, H.; Berndt, C.; Jones, D.P. Oxidative Stress. Annu. Rev. Biochem. 2017, 1, 715–748. [Google Scholar] [CrossRef] [PubMed]
- Wilson, D.M.; Cookson, M.R.; Van Den Bosch, L.; Zetterberg, H.; Holtzman, D.M.; Dewachter, I. Hallmarks of neurodegenerative diseases. Cell 2023, 4, 693–714. [Google Scholar] [CrossRef] [PubMed]
- Correia, A.S.; Cardoso, A.; Vale, N. Oxidative Stress in Depression: The Link with the Stress Response, Neuroinflammation, Serotonin, Neurogenesis and Synaptic Plasticity. Antioxidants 2023, 12, 470. [Google Scholar] [CrossRef] [PubMed]
- Olufunmilayo, E.O.; Gerke-Duncan, M.B.; Holsinger, R.M.D. Oxidative Stress and Antioxidants in Neurodegenerative Disorders. Antioxidants 2023, 12, 517. [Google Scholar] [CrossRef] [PubMed]
- Di Meo, S.; Reed, T.T.; Venditti, P.; Victor, V.M. Role of ROS and RNS Sources in Physiological and Pathological Conditions. Oxidative Med. Cell. Longev. 2016, 2016, 1245049. [Google Scholar] [CrossRef]
- Xian, H.; Watari, K.; Sanchez-Lopez, E.; Offenberger, J.; Onyuru, J.; Sampath, H.; Ying, W.; Hoffman, H.M.; Shadel, G.S.; Karin, M. Oxidized DNA fragments exit mitochondria via mPTP- and VDAC-dependent channels to activate NLRP3 inflammasome and interferon signaling. Immunity 2022, 8, 1370–1385.e8. [Google Scholar] [CrossRef]
- Hervera, A.; De Virgiliis, F.; Palmisano, I.; Zhou, L.; Tantardini, E.; Kong, G.; Hutson, T.; Danzi, M.C.; Perry, R.B.-T.; Santos, C.X.C.; et al. Reactive oxygen species regulate axonal regeneration through the release of exosomal NADPH oxidase 2 complexes into injured axons. Nat. Cell Biol. 2018, 3, 307–319. [Google Scholar] [CrossRef]
- Liu, Z.; Yao, X.; Jiang, W.; Li, W.; Zhu, S.; Liao, C.; Zou, L.; Ding, R.; Chen, J. Advanced oxidation protein products induce microglia-mediated neuroinflammation via MAPKs-NF-κB signaling pathway and pyroptosis after secondary spinal cord injury. J. Neuroinflamm. 2020, 1, 90. [Google Scholar] [CrossRef] [PubMed]
- Crotti, A.; Ransohoff, R.M. Microglial Physiology and Pathophysiology: Insights from Genome-wide Transcriptional Profiling. Immunity 2016, 3, 505–515. [Google Scholar] [CrossRef] [PubMed]
- David, S.; Kroner, A. Repertoire of microglial and macrophage responses after spinal cord injury. Nat. Rev. Neurosci. 2011, 7, 388–399. [Google Scholar] [CrossRef]
- Butovsky, O.; Weiner, H.L. Microglial signatures and their role in health and disease. Nat. Rev. Neurosci. 2018, 10, 622–635. [Google Scholar] [CrossRef] [PubMed]
- Chen, K.; Sun, S.; Wang, J.; Zhang, X.-D. Catalytic nanozymes for central nervous system disease. Coord. Chem. Rev. 2021, 432, 213751. [Google Scholar] [CrossRef]
- Martinelli, C.; Pucci, C.; Battaglini, M.; Marino, A.; Ciofani, G. Antioxidants and Nanotechnology: Promises and Limits of Potentially Disruptive Approaches in the Treatment of Central Nervous System Diseases. Adv. Healthc. Mater. 2020, 3, 1901589. [Google Scholar] [CrossRef]
- Huang, S.; Kou, X.; Shen, J.; Chen, G.; Ouyang, G. “Armor-Plating” Enzymes with Metal–Organic Frameworks (MOFs). Angew. Chem. Int. Ed. 2020, 23, 8786–8798. [Google Scholar] [CrossRef]
- Liu, Y.; Cheng, Y.; Zhang, H.; Zhou, M.; Yu, Y.; Lin, S.; Jiang, B.; Zhao, X.; Miao, L.; Wei, C.-W.; et al. Integrated cascade nanozyme catalyzes in vivo ROS scavenging for anti-inflammatory therapy. Sci. Adv. 2020, 29, eabb2695. [Google Scholar] [CrossRef]
- Chen, S.; Lu, W.; Xu, R.; Tan, J.; Liu, X. Pyrolysis-free and universal synthesis of metal-NC single-atom nanozymes with dual catalytic sites for cytoprotection. Carbon 2023, 201, 439–448. [Google Scholar] [CrossRef]
- Huang, G.; Zang, J.; He, L.; Zhu, H.; Huang, J.; Yuan, Z.; Chen, T.; Xu, A. Bioactive Nanoenzyme Reverses Oxidative Damage and Endoplasmic Reticulum Stress in Neurons under Ischemic Stroke. ACS Nano 2022, 1, 431–452. [Google Scholar] [CrossRef]
- Yuan, R.; Li, Y.; Han, S.; Chen, X.; Chen, J.; He, J.; Gao, H.; Yang, Y.; Yang, S.; Yang, Y. Fe-Curcumin Nanozyme-Mediated Reactive Oxygen Species Scavenging and Anti-Inflammation for Acute Lung Injury. ACS Cent. Sci. 2022, 1, 10–21. [Google Scholar] [CrossRef]
- Zhang, C.; Wang, X.; Du, J.; Gu, Z.; Zhao, Y. Reactive Oxygen Species-Regulating Strategies Based on Nanomaterials for Disease Treatment. Adv. Sci. 2021, 3, 2002797. [Google Scholar] [CrossRef]
- Nelson, K.M.; Dahlin, J.L.; Bisson, J.; Graham, J.; Pauli, G.F.; Walters, M.A. The Essential Medicinal Chemistry of Curcumin. J. Med. Chem. 2017, 5, 1620–1637. [Google Scholar] [CrossRef]
- Mohanty, C.; Sahoo, S.K. Curcumin and its topical formulations for wound healing applications. Drug Discov. Today 2017, 10, 1582–1592. [Google Scholar] [CrossRef]
- Wang, F.; Xia, J.-J.; Shen, L.-J.; Jiang, T.-T.; Li, W.-L.; You, D.-L.; Chang, Q.; Hu, S.-Y.; Wang, L.; Wu, X. Curcumin attenuates intracerebral hemorrhage-induced neuronal apoptosis and neuroinflammation by suppressing JAK1/STAT1 pathway. Biochem. Cell Biol. 2022, 3, 236–245. [Google Scholar] [CrossRef] [PubMed]
- Lee, S.; Cho, D.-C.; Han, I.; Kim, K.-T. Curcumin as a Promising Neuroprotective Agent for the Treatment of Spinal Cord Injury: A Review of the Literature. Neurospine 2022, 2, 249–261. [Google Scholar] [CrossRef] [PubMed]
- Yao, H.; Wang, F.; Chong, H.; Wang, J.; Bai, Y.; Du, M.; Yuan, X.; Yang, X.; Wu, M.; Li, Y.; et al. A Curcumin-Modified Coordination Polymers with ROS Scavenging and Macrophage Phenotype Regulating Properties for Efficient Ulcerative Colitis Treatment. Adv. Sci. 2023, 19, 2300601. [Google Scholar] [CrossRef] [PubMed]
- Jin, W.; Botchway, B.O.A.; Liu, X. Curcumin Can Activate the Nrf2/HO-1 Signaling Pathway and Scavenge Free Radicals in Spinal Cord Injury Treatment. Neurorehabilit. Neural Repair 2021, 7, 576–584. [Google Scholar] [CrossRef] [PubMed]
- Anand, P.; Kunnumakkara, A.B.; Newman, R.A.; Aggarwal, B.B. Bioavailability of Curcumin: Problems and Promises. Mol. Pharm. 2007, 6, 807–818. [Google Scholar] [CrossRef] [PubMed]
- Bi, Y.; Duan, W.; Chen, J.; You, T.; Li, S.; Jiang, W.; Li, M.; Wang, G.; Pan, X.; Wu, J.; et al. Neutrophil Decoys with Anti-Inflammatory and Anti-Oxidative Properties Reduce Secondary Spinal Cord Injury and Improve Neurological Functional Recovery. Adv. Funct. Mater. 2021, 34, 2102912. [Google Scholar] [CrossRef]
- Sun, D.; Liu, K.; Li, Y.; Xie, T.; Zhang, M.; Liu, Y.; Tong, H.; Guo, Y.; Zhang, Q.; Liu, H.; et al. Intrinsically Bioactive Manganese-Eumelanin Nanocomposites Mediated Antioxidation and Anti-Neuroinflammation for Targeted Theranostics of Traumatic Brain Injury. Adv. Healthc. Mater. 2022, 11, e2200517. [Google Scholar] [CrossRef] [PubMed]
- Wang, S.; Zheng, H.; Zhou, L.; Cheng, F.; Liu, Z.; Zhang, H.; Zhang, Q. Injectable redox and light responsive MnO2 hybrid hydrogel for simultaneous melanoma therapy and multidrug-resistant bacteria-infected wound healing. Biomaterials 2020, 260, 120314. [Google Scholar] [CrossRef]
- Yang, J.; Yang, B.; Shi, J. A Nanomedicine-Enabled Ion-Exchange Strategy for Enhancing Curcumin-Based Rheumatoid Arthritis Therapy. Angew. Chem. Int. Ed. 2023, 44, e202310061. [Google Scholar]
- Liu, C.; Hu, F.; Jiao, G.; Guo, Y.; Zhou, P.; Zhang, Y.; Zhang, Z.; Yi, J.; You, Y.; Li, Z.; et al. Dental pulp stem cell-derived exosomes suppress M1 macrophage polarization through the ROS-MAPK-NFκB P65 signaling pathway after spinal cord injury. J. Nanobiotechnol. 2022, 1, 65. [Google Scholar] [CrossRef] [PubMed]
- Gostner, J.M.; Becker, K.; Fuchs, D.; Sucher, R. Redox regulation of the immune response. Redox Rep. 2013, 3, 88–94. [Google Scholar] [CrossRef] [PubMed]
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Gao, F.; Liang, W.; Chen, Q.; Chen, B.; Liu, Y.; Liu, Z.; Xu, X.; Zhu, R.; Cheng, L. A Curcumin-Decorated Nanozyme with ROS Scavenging and Anti-Inflammatory Properties for Neuroprotection. Nanomaterials 2024, 14, 389. https://doi.org/10.3390/nano14050389
Gao F, Liang W, Chen Q, Chen B, Liu Y, Liu Z, Xu X, Zhu R, Cheng L. A Curcumin-Decorated Nanozyme with ROS Scavenging and Anti-Inflammatory Properties for Neuroprotection. Nanomaterials. 2024; 14(5):389. https://doi.org/10.3390/nano14050389
Chicago/Turabian StyleGao, Feng, Wenyu Liang, Qixin Chen, Bairu Chen, Yuchen Liu, Zhibo Liu, Xu Xu, Rongrong Zhu, and Liming Cheng. 2024. "A Curcumin-Decorated Nanozyme with ROS Scavenging and Anti-Inflammatory Properties for Neuroprotection" Nanomaterials 14, no. 5: 389. https://doi.org/10.3390/nano14050389