Impedimetric Sensing of Factor V Leiden Mutation by Zip Nucleic Acid Probe and Electrochemical Array
Abstract
:1. Introduction
2. Materials and Methods
2.1. Instruments and Chemicals
2.2. Methodology
- (i).
- Hybridization of the Z-probe with mutant type DNA, or wild type DNA, or C-mutant type DNA, T-mutant type DNA, ODN-1, ODN-2, mutant type, and wild type PCR in the solution phase;
- (ii).
- Immobilization of the hybridization products on the working electrode area of the array electrode;
- (iii).
- Impedimetric measurements.
2.3. Electrochemical Impedance Spectroscopy Measurements
3. Results
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Variable | Evaluated Conditions | Optimum Condition |
---|---|---|
Hybridization temperature, °C | 25, 50, 75 | 25 |
Hybridization buffer, pH | ABS (pH, 4.8) PBS (pH, 7.4) CBS (pH, 9.5) | PBS (pH 7.4) |
[Mg2+] in hybridization buffer, mM | NA *, 0.5, 1 | NA * |
Hybridization time, minute | 5, 10, 15 | 10 |
Rct (Ω) | HEff% | |
---|---|---|
Z-probe | 369.4 ± 59.9 | - |
Z-probe and mutant type DNA target | 3219.0 ± 373.0 | 89.0 |
Z-probe and wild type DNA target | 1083.0 ± 65.0 | 65.0 |
DNA probe | 848.0 ± 209.0 | - |
DNA probe and mutant type DNA Target | 2504.0 ± 629.3 | 66.0 |
DNA probe and wild type DNA target | 2327.0 ± 89.1 | 63.0 |
Rct (Ω) | HEff% | |
---|---|---|
Z-probe | 369.4 ± 59.9 | - |
Z-probe and mutant type PCR | 2608.0 ± 361.3 | 86.0 |
Z-probe and wild type PCR | 1923.3 ± 516.4 | 81.0 |
DNA probe | 848.0 ± 209.0 | - |
DNA probe and mutant type PCR | 1795.0 ± 481.2 | 53.0 |
DNA probe and wild type PCR | 1848.5 ± 379.7 | 54.0 |
Electrode | Modification | Analite | Technique | Assay Time | LOD | Reference |
---|---|---|---|---|---|---|
8-channel array of electrodes | Carbon nanofiber | DNA (FV Leiden mutation) | DPV | 135 min | 1.6 µM | [13] |
8-channel array of electrodes | Carbon nanofiber | DNA (FV Leiden mutation) | DPV | 35 min | 0.4 µM | [14] |
8-channel array of electrodes | Carbon nanofiber | DNA (FV Leiden mutation) | EIS | 50 min | 133.0 nM | [15] |
Gold film electrode array chip | - | DNA | SWV | NA | NA | [35] |
Three-dimensional interdigitated electrode array | Silane | DNA | EIS | 24 h | NA | [36] |
ITO electrode array | Graphene-mesoporous silica hybrid nanosheets | DNA | DPV | 70 min | 10.0 fM | [37] |
nanodisk-array electrodes | polystyrene-block-poly(methylmethacrylate)-derived thin films | DNA | CV | 2 h | 0.4 nM–4.2 nM | [38] |
32 microelectrode Chips (gold) | - | DNA | CV | 30 min | [39] | |
ITO electrode array on glass wafer | - | H1N1 influenza virus DNA | Capacitance | 3.5 h | 3.9 nM | [40] |
16 thru-hole array on printed circuit board | - | Hepatitis A-B-C virus DNA | ECL | 3.5 h | NA | [41] |
Gold electrode microarray | - | DNA | EIS | 22 h | 1.0 pM | [42] |
16-gold electrode sensor arrays | - | DNA | CV, Amperometry | 3 h | NA | [43] |
electrode array housed within the microfluidic cell | - | Karlodinium armiger DNA | SWV, Chronoamperometry | 17 h | 277.0 aM | [44] |
120-channel gold microelectrode array chip | - | miRNA | CV | 1 h | 140.0 zmol | [45] |
multi-electrode array (6-gold electrode) | - | HIV-1, HIV-2 DNA | SWV | 7.5 h | 0.1 nM | [46] |
16 microwells- column electrode | - | DNA | Amperometry | 2 h | 30.0 nM | [47] |
8-channel array of electrodes | Carbon nanofiber | DNA (FV Leiden mutation) | EIS | 25 min | 266.0 nM | Present work |
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Erdem, A.; Eksin, E. Impedimetric Sensing of Factor V Leiden Mutation by Zip Nucleic Acid Probe and Electrochemical Array. Biosensors 2020, 10, 116. https://doi.org/10.3390/bios10090116
Erdem A, Eksin E. Impedimetric Sensing of Factor V Leiden Mutation by Zip Nucleic Acid Probe and Electrochemical Array. Biosensors. 2020; 10(9):116. https://doi.org/10.3390/bios10090116
Chicago/Turabian StyleErdem, Arzum, and Ece Eksin. 2020. "Impedimetric Sensing of Factor V Leiden Mutation by Zip Nucleic Acid Probe and Electrochemical Array" Biosensors 10, no. 9: 116. https://doi.org/10.3390/bios10090116