Raman Microspectroscopic Analysis of Selenium Bioaccumulation by Green Alga Chlorella vulgaris
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cultivation of Se-Enriched C. vulgaris
2.2. Analysis of Se Accumulation Mechanism in Living and Dead Cells
2.3. Sample Collection and Preparation for ICP-MS and RS
2.4. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Analysis
2.5. Sample Handling Device
2.6. Raman Microspectroscopy
2.7. Scanning Electron Microscopy (SEM) and EDX Analyses
3. Results and Discussion
3.1. RS Quantification of Se in Algal Cells and Validation by ICP-MS
3.2. Comparative RS Analysis of Se Uptake Dynamics by Living and Dead Algal Cells
3.3. Verification of the Elemental Se Deposition in Algal Cells by SEM and EDX Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kizovský, M.; Pilát, Z.; Mylenko, M.; Hrouzek, P.; Kuta, J.; Skoupý, R.; Krzyžánek, V.; Hrubanová, K.; Adamczyk, O.; Ježek, J.; et al. Raman Microspectroscopic Analysis of Selenium Bioaccumulation by Green Alga Chlorella vulgaris. Biosensors 2021, 11, 115. https://doi.org/10.3390/bios11040115
Kizovský M, Pilát Z, Mylenko M, Hrouzek P, Kuta J, Skoupý R, Krzyžánek V, Hrubanová K, Adamczyk O, Ježek J, et al. Raman Microspectroscopic Analysis of Selenium Bioaccumulation by Green Alga Chlorella vulgaris. Biosensors. 2021; 11(4):115. https://doi.org/10.3390/bios11040115
Chicago/Turabian StyleKizovský, Martin, Zdeněk Pilát, Mykola Mylenko, Pavel Hrouzek, Jan Kuta, Radim Skoupý, Vladislav Krzyžánek, Kamila Hrubanová, Olga Adamczyk, Jan Ježek, and et al. 2021. "Raman Microspectroscopic Analysis of Selenium Bioaccumulation by Green Alga Chlorella vulgaris" Biosensors 11, no. 4: 115. https://doi.org/10.3390/bios11040115