Double Competitive Immunodetection of Small Analyte: Realization for Highly Sensitive Lateral Flow Immunoassay of Chloramphenicol
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Materials
2.2. Equipment
2.3. Synthesis of Hapten-Protein Conjugates
2.4. Synthesis of Gold Nanoparticles (GNP) and Their Conjugates with Monoclonal Antibodies to CAP
2.5. Assembling of Test Strips for LFIA
2.6. Lateral Flow Assay of CAP
3. Results
3.1. Mathematical Description of Competitive LFIA Schemes
3.2. Optimization of LFIA Parameters to Perform Assay of CAP
3.3. Assessment of the Developed Double Competitive LFIA in Honey Samples
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sotnikov, D.V.; Barshevskaya, L.V.; Bartosh, A.V.; Zherdev, A.V.; Dzantiev, B.B. Double Competitive Immunodetection of Small Analyte: Realization for Highly Sensitive Lateral Flow Immunoassay of Chloramphenicol. Biosensors 2022, 12, 343. https://doi.org/10.3390/bios12050343
Sotnikov DV, Barshevskaya LV, Bartosh AV, Zherdev AV, Dzantiev BB. Double Competitive Immunodetection of Small Analyte: Realization for Highly Sensitive Lateral Flow Immunoassay of Chloramphenicol. Biosensors. 2022; 12(5):343. https://doi.org/10.3390/bios12050343
Chicago/Turabian StyleSotnikov, Dmitriy V., Lyubov V. Barshevskaya, Anastasia V. Bartosh, Anatoly V. Zherdev, and Boris B. Dzantiev. 2022. "Double Competitive Immunodetection of Small Analyte: Realization for Highly Sensitive Lateral Flow Immunoassay of Chloramphenicol" Biosensors 12, no. 5: 343. https://doi.org/10.3390/bios12050343