Analysis of the Interaction between DNA Aptamers and Cytochrome C on the Surface of Lipid Films and on the MUA Monolayer: A QCM-D Study †
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Synthesis of Gold Nanowires
2.3. Functionalization of AuNWs with DNA Aptamers
2.4. Piezocrystal Cleaning, Preparation of Lipid Films, and MUA Monolayers with Covalently Attached Cyt C
2.5. The Principles of QCM-D and Viscoelastic Properties of Lipid Layers with Cyt C
3. Results and Discussion
3.1. The Interaction of Cytochrome C with Supported Lipid Films and with DNA Aptamers
3.2. The Interaction of DNA Aptamers with Cytochrome C Covalently Attached to the 11-Mercaptoundecanoic Layer
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameter | Composition of the Adlayer | ||
---|---|---|---|
Lipid Film | Cyt C | DNA Aptamer | |
Δf, Hz | −150 | −77 | 70 |
ΔD, 10−5 | 3.17 | 1.18 | 1.11 |
Δm/A, ng/cm2 | 1035 | 531.3 | −483 |
h, nm | 10.14 | 3.9 | 3.3 |
σ, 1013 cm−2 | 92.7 | 2.67 | - |
ΔΓ, nm | 19.4 | 11.7 | 61.6 |
Δη, mPa·s | 0.1 | 0.05 | 1.53 |
Δμ, 105 Pa | 5.72 | 5.63 | 4.91 |
Changes in Frequency and Surface Density | Cyt C, μM | |||
---|---|---|---|---|
0.5 | 2 | 4.5 | 10 | |
Δf, Hz | −21.16 ± 6.48 | −33.60 ± 6.95 | −23.30 ± 3.25 | −22.66 ± 2.31 |
Δm/A, ng/cm2 | 146.0 ± 44.7 | 231.8 ± 48.0 | 160.8 ± 22.4 | 156.35 ± 15.9 |
h, nm | 1.08 ± 0.33 | 1.72 ± 0.36 | 1.19 ± 0.17 | 1.16 ± 0.12 |
σ, 1012 cm−2 | 7.33 ± 2.24 | 11.64 ± 2.41 | 8.10 ± 1.13 | 7.84 ± 0.80 |
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Tatarko, M.; Spagnolo, S.; Csiba, M.; Šubjaková, V.; Hianik, T. Analysis of the Interaction between DNA Aptamers and Cytochrome C on the Surface of Lipid Films and on the MUA Monolayer: A QCM-D Study. Biosensors 2023, 13, 251. https://doi.org/10.3390/bios13020251
Tatarko M, Spagnolo S, Csiba M, Šubjaková V, Hianik T. Analysis of the Interaction between DNA Aptamers and Cytochrome C on the Surface of Lipid Films and on the MUA Monolayer: A QCM-D Study. Biosensors. 2023; 13(2):251. https://doi.org/10.3390/bios13020251
Chicago/Turabian StyleTatarko, Marek, Sandro Spagnolo, Martin Csiba, Veronika Šubjaková, and Tibor Hianik. 2023. "Analysis of the Interaction between DNA Aptamers and Cytochrome C on the Surface of Lipid Films and on the MUA Monolayer: A QCM-D Study" Biosensors 13, no. 2: 251. https://doi.org/10.3390/bios13020251