Simple, Fast and Convenient Magnetic Bead-Based Sample Preparation for Detecting Viruses via Raman-Spectroscopy
Abstract
:1. Introduction
2. Methods
2.1. Preparation of ACE2 Beads
2.2. Virus Isolation
2.3. Raman Measurements
2.4. Data Processing
2.5. Cell Culture and Viral Propagation
2.6. Virus Lysis and qRT-PCR
3. Results and Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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(a) NCC Coefficient | ||||
SARS-CoV-2 Mean ± Sd | Influenza A Virus Mean ± Sd | Mean Value | p-Value Bold: Significant at α = 0.05 | |
Data Set 1 | ||||
Replicate 1 | 0.977 ± 0.028 | 0.951 ± 0.104 | 0.0261 | |
Replicate 2 | 0.966 ± 0.097 | 0.960 ± 0.068 | 0.0889 | |
Replicate 3 | 0.986 ± 0.018 | 0.970 ± 0.040 | 0.0340 | |
Replicate 4 | 0.990 ± 0.015 | 0.987 ± 0.016 | 0.0154 | |
Data Set 2 | ||||
Replicate 1 | 0.996 ± 0.004 | 0.994 ± 0.007 | 0.0258 | |
Replicate 2 | 0.988 ± 0.013 | 0.974 ± 0.048 | 2.93 × 10−8 | |
Replicate 3 | 0.990 ± 0.009 | 0.985 ± 0.009 | 3.08 × 10−7 | |
Replicate 4 | 0.987 ± 0.058 | 0.983 ± 0.088 | 0.944 | |
(b) Pearson coefficient | ||||
Data Set 1 | ||||
Replicate 1 | 0.939 ± 0.076 | 0.886 ± 0.188 | 0.0682 | |
Replicate 2 | 0.928 ± 0.140 | 0.902 ± 0.154 | 0.0889 | |
Replicate 3 | 0.965 ± 0.049 | 0.919 ± 0.108 | 0.0198 | |
Replicate 4 | 0.973 ± 0.038 | 0.965 ± 0.043 | 0.0154 | |
Data Set 2 | ||||
Replicate 1 | 0.988 ± 0.010 | 0.984 ± 0.018 | 0.0258 | |
Replicate 2 | 0.966 ± 0.035 | 0.934 ± 0.111 | 4.60 × 10−5 | |
Replicate 3 | 0.973 ± 0.038 | 0.957 ± 0.025 | 1.22 × 10−7 | |
Replicate 4 | 0.970 ± 0.105 | 0.969 ± 0.118 | 0.944 | |
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Pahlow, S.; Richard-Lacroix, M.; Hornung, F.; Köse-Vogel, N.; Mayerhöfer, T.G.; Hniopek, J.; Ryabchykov, O.; Bocklitz, T.; Weber, K.; Ehricht, R.; et al. Simple, Fast and Convenient Magnetic Bead-Based Sample Preparation for Detecting Viruses via Raman-Spectroscopy. Biosensors 2023, 13, 594. https://doi.org/10.3390/bios13060594
Pahlow S, Richard-Lacroix M, Hornung F, Köse-Vogel N, Mayerhöfer TG, Hniopek J, Ryabchykov O, Bocklitz T, Weber K, Ehricht R, et al. Simple, Fast and Convenient Magnetic Bead-Based Sample Preparation for Detecting Viruses via Raman-Spectroscopy. Biosensors. 2023; 13(6):594. https://doi.org/10.3390/bios13060594
Chicago/Turabian StylePahlow, Susanne, Marie Richard-Lacroix, Franziska Hornung, Nilay Köse-Vogel, Thomas G. Mayerhöfer, Julian Hniopek, Oleg Ryabchykov, Thomas Bocklitz, Karina Weber, Ralf Ehricht, and et al. 2023. "Simple, Fast and Convenient Magnetic Bead-Based Sample Preparation for Detecting Viruses via Raman-Spectroscopy" Biosensors 13, no. 6: 594. https://doi.org/10.3390/bios13060594