A Prototype Assay Multiplexing SARS-CoV-2 3CL-Protease and Angiotensin-Converting Enzyme 2 for Saliva-Based Diagnostics in COVID-19
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Assay Buffers I and II
2.3. Preparation of Protease Standards
2.4. Preparation of Magnetic Beads
2.5. Preparation of ACE2 Fluorescent Substrate
2.6. 3CL Protease Assay
2.7. ACE2 Assay
2.8. 30-Minute Sequential ACE2-3CL Assay
2.9. Raman Spectroscopy
2.10. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)
2.11. Collection of Clinical Samples (IRB, etc.)
3. Results
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Suresh, V.; Sheik, D.A.; Detomasi, T.C.; Zhao, T.; Zepeda, T.; Saladi, S.; Rajesh, U.C.; Byers, K.; Craik, C.S.; Davisson, V.J. A Prototype Assay Multiplexing SARS-CoV-2 3CL-Protease and Angiotensin-Converting Enzyme 2 for Saliva-Based Diagnostics in COVID-19. Biosensors 2023, 13, 682. https://doi.org/10.3390/bios13070682
Suresh V, Sheik DA, Detomasi TC, Zhao T, Zepeda T, Saladi S, Rajesh UC, Byers K, Craik CS, Davisson VJ. A Prototype Assay Multiplexing SARS-CoV-2 3CL-Protease and Angiotensin-Converting Enzyme 2 for Saliva-Based Diagnostics in COVID-19. Biosensors. 2023; 13(7):682. https://doi.org/10.3390/bios13070682
Chicago/Turabian StyleSuresh, Vallabh, Daniel A. Sheik, Tyler C. Detomasi, Tianqi Zhao, Theresa Zepeda, Shyam Saladi, Ummadisetti Chinna Rajesh, Kaleb Byers, Charles S. Craik, and Vincent Jo Davisson. 2023. "A Prototype Assay Multiplexing SARS-CoV-2 3CL-Protease and Angiotensin-Converting Enzyme 2 for Saliva-Based Diagnostics in COVID-19" Biosensors 13, no. 7: 682. https://doi.org/10.3390/bios13070682