Solid-Phase Electrochemiluminescence Enzyme Electrodes Based on Nanocage Arrays for Highly Sensitive Detection of Cholesterol
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Materials
2.2. Measurements and Instrumentations
2.3. Preparation of bp-VMSF-Modified Electrode
2.4. Preparation of Solid-Phase ECL Enzyme-Sensing Platform
2.5. ECL Detection of Cholesterol
3. Results and Discussion
3.1. Characterization of bp-VMSF
3.2. Stability of Ru(bpy)32+ Fixed in bp-VMSF
3.3. Feasibility for the Fabrication of Enzyme-Immobilized Electrodes
3.4. Feasibility of ECL Detection of Cholesterol Using Enzyme-Immobilized Electrode
3.5. Performance for ECL Detection of Cholesterol
3.6. Selectivity of the Fabricated Sensors
3.7. Real Sample Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Analyte | Added (mM) | Detected (mM) | RSD (%, n = 3) | Recovery (%) |
---|---|---|---|---|
cholesterol | 0.50 | 0.55 | 2.1 | 110.0 |
1.00 | 1.09 | 1.2 | 109.0 | |
4.50 | 4.47 | 0.2 | 99.3 |
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Ma, X.; Zhang, Z.; Zheng, Y.; Liu, J. Solid-Phase Electrochemiluminescence Enzyme Electrodes Based on Nanocage Arrays for Highly Sensitive Detection of Cholesterol. Biosensors 2024, 14, 403. https://doi.org/10.3390/bios14080403
Ma X, Zhang Z, Zheng Y, Liu J. Solid-Phase Electrochemiluminescence Enzyme Electrodes Based on Nanocage Arrays for Highly Sensitive Detection of Cholesterol. Biosensors. 2024; 14(8):403. https://doi.org/10.3390/bios14080403
Chicago/Turabian StyleMa, Xinying, Zhe Zhang, Yanyan Zheng, and Jiyang Liu. 2024. "Solid-Phase Electrochemiluminescence Enzyme Electrodes Based on Nanocage Arrays for Highly Sensitive Detection of Cholesterol" Biosensors 14, no. 8: 403. https://doi.org/10.3390/bios14080403