Applying a Fluorescence Polarization Assay for Detection of Brucellosis in Animals Using the Fluorescently Labeled Synthetic Oligosaccharides as Biosensing Tracer
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Equipment
2.2. Synthesis of Fluorescein-Labeled Oligosaccharide Tracers 1b–3b
2.3. Serum Samples
- (a)
- Brucellosis positive (N = 93)—serum samples from several brucellosis-unfavorable farms with a positive reaction in at least two of the used serological tests.
- (b)
- Brucellosis negative (N = 17)—the reaction to all serological tests was negative for brucellosis.
2.4. Fluorescence Polarization Assay
2.5. Data Analysis
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Khurana, S.K.; Sehrawat, A.; Tiwari, R.; Prasad, M.; Gulati, B.; Shabbir, M.Z.; Chhabra, R.; Karthik, K.; Patel, S.K.; Pathak, M.; et al. Bovine Brucellosis—A Comprehensive Review. Vet. Q. 2021, 41, 61–88. [Google Scholar] [CrossRef]
- Smirnova, E.A.; Vasin, A.V.; Sandybaev, N.T.; Klotchenko, S.A.; Plotnikova, M.A.; Chervyakova, O.V.; Sansyzbay, A.R.; Kiselev, O.I. Current Methods of Human and Animal Brucellosis Diagnostics. Adv. Infect. Dis. 2013, 3, 177–184. [Google Scholar] [CrossRef]
- Saadat, S.; Mardaneh, J.; Ahouran, M.; Mohammadzadeh, A.; Ardebili, A.; Yousefi, M. Diagnosis of Cattle Brucellosis by PCR and Serological Methods: Comparison of Diagnostic Tests. Biomed. Pharmacol. J. 2017, 14, 881–888. [Google Scholar] [CrossRef]
- Mantur, B.; Amarnath, S.; Shinde, R. Review of Clinical and Laboratory Features of Human Brucellosis. Indian. J. Med. Microbiol. 2007, 25, 188–202. [Google Scholar] [CrossRef]
- Rahman, A.K.M.A.; Smit, S.; Devleesschauwer, B.; Kostoulas, P.; Abatih, E.; Saegerman, C.; Shamsuddin, M.; Berkvens, D.; Dhand, N.K.; Ward, M.P. Bayesian Evaluation of Three Serological Tests for the Diagnosis of Bovine Brucellosis in Bangladesh. Epidemiol. Infect. 2019, 147, e73. [Google Scholar] [CrossRef]
- Ducrotoy, M.J.; Muñoz, P.M.; Conde-Álvarez, R.; Blasco, J.M.; Moriyón, I. A Systematic Review of Current Immunological Tests for the Diagnosis of Cattle Brucellosis. Prev. Vet. Med. 2018, 151, 57–72. [Google Scholar] [CrossRef]
- Nielsen, K.; Yu, W.L. Serological Diagnosis of Brucellosis. Prilozi 2010, 31, 65–89. [Google Scholar]
- Ibarra, M.; Campos, M.; Hernán, B.; Loor-Giler, A.; Chamorro, A.; Nuñez, L. Comparison of Diagnostic Tests for Detecting Bovine Brucellosis in Animals Vaccinated with S19 and RB51 Strain Vaccines. Vet. World 2023, 16, 2080–2085. [Google Scholar] [CrossRef] [PubMed]
- Lu, J.; Li, C.; Zhang, E.; Hou, S.; Xiao, K.; Li, X.; Zhang, L.; Wang, Z.; Chen, C.; Li, T. Novel Vertical Flow Immunoassay with Au@ PtNPs for Rapid, Ultrasensitive, and On-Site Diagnosis of Human Brucellosis. ACS Omega 2023, 8, 29534–29542. [Google Scholar] [CrossRef]
- Baldi, P.C.; Giambartolomei, G.H.; Goldbaum, F.A.; Abdón, L.F.; Velikovsky, C.A.; Kittelberger, R.; Fossati, C.A. Humoral Immune Response against Lipopolysaccharide and Cytoplasmic Proteins of Brucella Abortus in Cattle Vaccinated with B. Abortus S19 or Experimentally Infected with Yersinia Enterocolitica Serotype 0:9. Clin. Diagn. Lab. Immunol. 1996, 3, 472–476. [Google Scholar] [CrossRef]
- Ran, X.; Cheng, J.; Wang, M.; Chen, X.; Wang, H.; Ge, Y.; Ni, H.; Zhang, X.-X.; Wen, X. Brucellosis Seroprevalence in Dairy Cattle in China during 2008–2018: A Systematic Review and Meta-Analysis. Acta Trop. 2019, 189, 117–123. [Google Scholar] [CrossRef]
- Nielsen, K.; Lin, M.; Gall, D.; Jolley, M. Fluorescence Polarization Immunoassay: Detection of Antibody to Brucella Abortus. Methods 2000, 22, 71–76. [Google Scholar] [CrossRef]
- Mukhametova, L.I.; Krylov, V.B.; Solovev, A.S.; Yashunsky, D.V.; Matveev, A.L.; Tikunova, N.V.; Eremin, S.A.; Nifantiev, N.E. Affinity Characteristics of Anti-β-(1→3)-D-Glucan Monoclonal Antibody 3G11 by Fluorescence Polarization Immunoassay. Russ. Chem. Bull. 2021, 70, 975–981. [Google Scholar] [CrossRef]
- Mukhametova, L.I.; Zherdev, D.O.; Kuznetsov, A.N.; Yudina, O.N.; Tsvetkov, Y.E.; Eremin, S.A.; Krylov, V.B.; Nifantiev, N.E. Fluorescence-Polarization-Based Assaying of Lysozyme with Chitooligosaccharide Tracers. Biomolecules 2024, 14, 170. [Google Scholar] [CrossRef]
- Cupp-Enyard, C. Use of the Protease Fluorescent Detection Kit to Determine Protease Activity. J. Vis. Exp. 2009, 30, 1514. [Google Scholar] [CrossRef]
- Chapter 3.1.4. Brucellosis (infection with B. Abortus, B. Melitensis and B. Suis). WOAH Terrestrial Manual 2022. Available online: https://www.woah.org/app/uploads/2024/01/a-73sg-12-cs2b.pdf (accessed on 1 August 2024).
- Report of the Meeting of the OIE Biological Standards Commission. In Proceedings of the 73rd General Session International Committee World Organization for Animal Health, Paris, France, 26–28 January 2005; Available online: https://www.woah.org/fileadmin/Home/eng/Health_standards/tahm/3.01.04_BRUCELLOSIS.pdf (accessed on 1 August 2024).
- Dajer, A.; Luna-Martínez, E.; Zapata, D.; Villegas, S.; Gutiérrez, E.; Peña, G.; Gurría, F.; Nielsen, K.; Gall, D. Evaluation of a Fluorescence-Polarization Assay for the Diagnosis of Bovine Brucellosis in México. Prev. Vet. Med. 1999, 40, 67–73. [Google Scholar] [CrossRef]
- Minas, A.; Stournara, A.; Minas, M.; Papaioannou, A.; Krikelis, V.; Tselepidis, S. Validation of Fluorescence Polarization Assay (FPA) and Comparison with Other Tests Used for Diagnosis of B. Melitensis Infection in Sheep. Vet. Microbiol. 2005, 111, 211–221. [Google Scholar] [CrossRef]
- Nielsen, K.; Gall, D.; Smith, P.; Vigliocco, A.; Perez, B.; Samartino, L.; Nicoletti, P.; Dajer, A.; Elzer, P.; Enright, F. Validation of the Fluorescence Polarization Assay as a Serological Test for the Presumptive Diagnosis of Porcine Brucellosis. Vet. Microbiol. 1999, 68, 245–253. [Google Scholar] [CrossRef]
- Gall, D.; Nielsen, K.; Forbes, L.; Cook, W.; Leclair, D.; Balsevicius, S.; Kelly, L.; Smith, P.; Mallory, M. Evaluation of the Fluorescence Polarization Assay and Comparison to Other Serological Assays for Detection of Brucellosis in Cervids. J. Wildl. Dis. 2001, 37, 110–118. [Google Scholar] [CrossRef]
- Gwida, M.M.; El-Gohary, A.H.; Melzer, F.; Tomaso, H.; Rösler, U.; Wernery, U.; Wernery, R.; Elschner, M.C.; Khan, I.; Eickhoff, M.; et al. Comparison of Diagnostic Tests for the Detection of Brucella Spp. in Camel Sera. BMC Res. Notes 2011, 4, 525. [Google Scholar] [CrossRef]
- Lucero, N.E.; Escobar, G.I.; Ayala, S.M.; Paulo, P.S.; Nielsen, K. Fluorescence Polarization Assay for Diagnosis of Human Brucellosis. J. Med. Microbiol. 2003, 52, 883–887. [Google Scholar] [CrossRef]
- Konstantinidis, A.; Minas, A.; Pournaras, S.; Kansouzidou, A.; Papastergiou, P.; Maniatis, A.; Stathakis, N.; Hadjichristodoulou, C. Evaluation and Comparison of Fluorescence Polarization Assay with Three of the Currently Used Serological Tests in Diagnosis of Human Brucellosis. Eur. J. Clin. Microbiol. Infect. Dis. 2007, 26, 715–721. [Google Scholar] [CrossRef]
- Lin, M.; Nielsen, K. Binding of the Brucella Abortus Lipopolysaccharide O-Chain Fragment to a Monoclonal Antibody. Quantitative Analysis by Fluorescence Quenching and Polarization. J. Biol. Chem. 1997, 272, 2821–2827. [Google Scholar] [CrossRef]
- Nielsen, K.; Gall, D.; Smith, P.; Kelly, W.; Yeo, J.; Kenny, K.; Heneghan, T.; McNamara, S.; Maher, P.; O’Connor, J.; et al. Fluorescence Polarization Assay for the Diagnosis of Bovine Brucellosis: Adaptation to Field Use. Vet. Microbiol. 2001, 80, 163–170. [Google Scholar] [CrossRef]
- Argunov, D.A.; Krylov, V.B.; Nifantiev, N.E. Convergent synthesis of isomeric heterosaccharides related to the fragments of galactomannan from Aspergillus fumigatus. Org. Biomol. Chem. 2015, 13, 3255–3267. [Google Scholar] [CrossRef]
- Matveev, A.L.; Krylov, V.B.; Emelyanova, L.A.; Solovev, A.S.; Khlusevich, Y.A.; Baykov, I.K.; Fontaine, T.; Latgé, J.-P.; Tikunova, N.V.; Nifantiev, N.E. Novel mouse monoclonal antibodies specifically recognize Aspergillus fumigatus galactomannan. PLoS ONE 2018, 13, e0193938. [Google Scholar] [CrossRef]
- Krylov, V.B.; Solovev, A.S.; Argunov, D.A.; Latgé, J.-P.; Nifantiev, N.E. Reinvestigation of Carbohydrate Specificity of EB-A2 Monoclonal Antibody Used in the Immune Detection of Aspergillus Fumigatus Galactomannan. Heliyon 2019, 5, e01173. [Google Scholar] [CrossRef]
- Tsvetkov, Y.E.; Nifantiev, N.E. Synthesis of a Spacer-Armed Disaccharide Structurally Related to the M Antigenic Fragment of Brucella O-Polysaccharides. Russ. Chem. Bull. 2023, 72, 2731–2737. [Google Scholar] [CrossRef]
- Tsvetkov, Y.E.; Volkov, T.M.; Eremin, S.A.; Sklyarov, O.D.; Kulakov, Y.K.; Krylov, V.B.; Nifantiev, N.E. New Synthesis of oligosaccharides modelling the M epitope of the Brucella O-polysaccharide. Front. Chem. 2024, 12, 1424157. [Google Scholar] [CrossRef]
- McGiven, J.; Howells, L.; Duncombe, L.; Stack, J.; Ganesh, N.V.; Guiard, J.; Bundle, D.R. Improved Serodiagnosis of Bovine Brucellosis by Novel Synthetic Oligosaccharide Antigens Representing the Capping M Epitope Elements of Brucella O-Polysaccharide. J. Clin. Microbiol. 2015, 53, 1204–1210. [Google Scholar] [CrossRef]
- Duncombe, L.; Howells, L.; Haughey, A.; Taylor, A.V.; Kaveh, D.; Erdenliğ Gϋrbilek, S.; Dell, A.; Hitchen, P.G.; Haslam, S.M.; Mandal, S.S.; et al. The Tip of Brucella O-Polysaccharide Is a Potent Epitope in Response to Brucellosis Infection and Enables Short Synthetic Antigens to Be Superior Diagnostic Reagents. Microorganisms 2022, 10, 708. [Google Scholar] [CrossRef] [PubMed]
Characteristic | Group | Fluorescence Polarization (mP) for Tracers | ||
---|---|---|---|---|
1b | 2b | 3b | ||
Range | Bru(+) | 50.5–195.7 | 57.2–200.6 | 62.2–192.2 |
Bru(−) | 58.2–94.1 | 53.5–98.0 | 54.5–74.0 | |
Median (LQ–UQ *) | Bru(+) | 89.4 (65.4–128.8) | 85.6 (70.7–119.8) | 78.0 (72.8–85.4) |
Bru(−) | 68.2 (62.3–76.9) | 69.0 (68.0–80.0) | 68.0 (66.5–70.6) |
Total | Results | AT | CFT | RBT | FPA 1b | FPA 2b | FPA 3b |
---|---|---|---|---|---|---|---|
110 | Positive | 75 | 87 | 84 | 59 | 66 | 83 |
Questionable | 11 | 0 | - | - | - | - | |
Negative | 24 | 23 | 26 | 51 | 44 | 27 |
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Mukhametova, L.I.; Zherdev, D.O.; Eremin, S.A.; Kuznetsov, A.N.; Yudin, V.I.; Sclyarov, O.D.; Babicheva, O.V.; Motorygin, A.V.; Tsvetkov, Y.E.; Krylov, V.B.; et al. Applying a Fluorescence Polarization Assay for Detection of Brucellosis in Animals Using the Fluorescently Labeled Synthetic Oligosaccharides as Biosensing Tracer. Biosensors 2024, 14, 404. https://doi.org/10.3390/bios14080404
Mukhametova LI, Zherdev DO, Eremin SA, Kuznetsov AN, Yudin VI, Sclyarov OD, Babicheva OV, Motorygin AV, Tsvetkov YE, Krylov VB, et al. Applying a Fluorescence Polarization Assay for Detection of Brucellosis in Animals Using the Fluorescently Labeled Synthetic Oligosaccharides as Biosensing Tracer. Biosensors. 2024; 14(8):404. https://doi.org/10.3390/bios14080404
Chicago/Turabian StyleMukhametova, Liliya I., Dmitry O. Zherdev, Sergei A. Eremin, Anton N. Kuznetsov, Viktor I. Yudin, Oleg D. Sclyarov, Olesia V. Babicheva, Anton V. Motorygin, Yury E. Tsvetkov, Vadim B. Krylov, and et al. 2024. "Applying a Fluorescence Polarization Assay for Detection of Brucellosis in Animals Using the Fluorescently Labeled Synthetic Oligosaccharides as Biosensing Tracer" Biosensors 14, no. 8: 404. https://doi.org/10.3390/bios14080404