Protein-Flavonoid Interaction Studies by a Taylor Dispersion Surface Plasmon Resonance (SPR) Technique: A Novel Method to Assess Biomolecular Interactions
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials
2.2. SPR Measurements
2.3. Data Processing
3. Results and Discussion
Protein-Flavonoid Interactions
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Flavonoids | ka (M−1s−1) | kd (s−1) | KD (M) | |
---|---|---|---|---|
Quercetin | Site 1 | 2.40 ± 0.01 × 105 | 0.016 ± 0.003 | 6.30 ± 0.03 × 10−8 |
Site 2 | 1.00 ± 0.10 × 102 | 0.405 ± 0.004 | >5.00 × 10−4 | |
Luteolin | Site 1 | 3.20 ± 0.30 × 103 | 0.205 ± 0.005 | 6.34 ± 0.01 × 10−5 |
Site 2 | 1.71 ± 0.01 × 101 | 0.007 ± 0.001 | 4.06 ± 0.02 × 10−4 | |
Resveratrol | Site 1 | 7.00 ± 0.20 × 103 | 0.003± 0.002 | 4.00 ± 0.10 × 10−7 |
Site 2 | 2.90 ± 0.60 × 103 | 0.520 ± 0.003 | 1.80 ± 0.40 × 10−4 | |
Kaempferol | Site 1 | 4.84 ± 0.06 × 104 | 0.002 ± 0.002 | 3.70 ± 0.07 × 10−8 |
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Vachali, P.P.; Li, B.; Besch, B.M.; Bernstein, P.S. Protein-Flavonoid Interaction Studies by a Taylor Dispersion Surface Plasmon Resonance (SPR) Technique: A Novel Method to Assess Biomolecular Interactions. Biosensors 2016, 6, 6. https://doi.org/10.3390/bios6010006
Vachali PP, Li B, Besch BM, Bernstein PS. Protein-Flavonoid Interaction Studies by a Taylor Dispersion Surface Plasmon Resonance (SPR) Technique: A Novel Method to Assess Biomolecular Interactions. Biosensors. 2016; 6(1):6. https://doi.org/10.3390/bios6010006
Chicago/Turabian StyleVachali, Preejith P., Binxing Li, Brian M. Besch, and Paul S. Bernstein. 2016. "Protein-Flavonoid Interaction Studies by a Taylor Dispersion Surface Plasmon Resonance (SPR) Technique: A Novel Method to Assess Biomolecular Interactions" Biosensors 6, no. 1: 6. https://doi.org/10.3390/bios6010006