A Highly Active Chimeric Lysin with a Calcium-Enhanced Bactericidal Activity against Staphylococcus aureus In Vitro and In Vivo
Abstract
:1. Introduction
2. Results
2.1. Construction and Purification of ClyC
2.2. Bactericidal Activity of ClyC
2.3. Characteristics of ClyC
2.4. Effects of Calcium on the Enzymatic Activity of ClyC
2.5. Effects of ClyC Alone against Planktonic and Sessile S. aureus or in Combination with Penicillin G
2.6. ClyC Cytotoxicity In Vitro
2.7. ClyC Protects Mice from Lethal S. aureus Infection
3. Discussion
4. Materials and Methods
4.1. Ethical Consideration
4.2. Bacterial Strains
4.3. Construction of Expression Plasmids and Protein Purification
4.4. Lytic Activity Assay
4.5. Lyophilization
4.6. MIC of ClyC Alone and in Combination with Penicillin G
4.7. Effects of Different Factors on ClyC Activity
4.8. Construction of 3D Models of ClyC
4.9. ClyC Cytotoxicity Assay
4.9.1. Biofilm Removal Efficacy of ClyC
4.9.2. Mouse Infection Model
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Li, X.; Wang, S.; Nyaruaba, R.; Liu, H.; Yang, H.; Wei, H. A Highly Active Chimeric Lysin with a Calcium-Enhanced Bactericidal Activity against Staphylococcus aureus In Vitro and In Vivo. Antibiotics 2021, 10, 461. https://doi.org/10.3390/antibiotics10040461
Li X, Wang S, Nyaruaba R, Liu H, Yang H, Wei H. A Highly Active Chimeric Lysin with a Calcium-Enhanced Bactericidal Activity against Staphylococcus aureus In Vitro and In Vivo. Antibiotics. 2021; 10(4):461. https://doi.org/10.3390/antibiotics10040461
Chicago/Turabian StyleLi, Xiaohong, Shujuan Wang, Raphael Nyaruaba, Huan Liu, Hang Yang, and Hongping Wei. 2021. "A Highly Active Chimeric Lysin with a Calcium-Enhanced Bactericidal Activity against Staphylococcus aureus In Vitro and In Vivo" Antibiotics 10, no. 4: 461. https://doi.org/10.3390/antibiotics10040461