The Genetic Characteristics and Carbapenem Resistance Mechanism of ST307 Klebsiella pneumoniae Coharbouring blaCMY-6, blaOXA-48, and a Truncated blaNDM-1
Abstract
:1. Introduction
2. Results
2.1. Clonality Analysis and Population Structure of ST307
2.2. Antimicrobial Resistance Genes in the Isolates
2.3. Difference in pNDM-1 and pOXA-48 among These Isolates
2.4. Conjugation Assay and Gene Cloning Experiments
3. Discussion
4. Materials and Methods
4.1. Collection of Isolates and Bacterial Identification
4.2. Antimicrobial Resistance Testing
4.3. PFGE, S1-PFGE, and Southern Blotting
4.4. Whole-Genome Sequencing and Subsequent Analysis
4.5. Conjugation Experiments
4.6. Plasmid Construction
4.7. Nucleotide Sequence Accession Numbers
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Isolates | Department | Specimen Type | ST | MIC (mg/L) 1 | Beta-Lactamases | ||||
---|---|---|---|---|---|---|---|---|---|
ATM | IPM | MEM | ETP | CAZ | |||||
CHN24001 | Department of Burns | Blood | 307 | >64 | 64 | 64 | >64 | >64 | blaNDM-12, blaOXA-48, blaCMY-6, blaCTX-M-15, blaSHV-106, blaTEM-1, blaOXA-1 |
CHN24003 | Department of Burns | Secretion | 307 | >64 | 16 | 16 | >64 | >64 | blaNDM-12, blaOXA-48, blaCMY-6, blaSHV-106, blaTEM-1 |
CHN24025 | Department of Burns | Secretion | 307 | >64 | 32 | 32 | >64 | >64 | blaNDM-12, blaOXA-48, blaCMY-6, blaSHV-106, blaTEM-1 |
CHN24039 | Department of Burns | Secretion | 307 | >64 | 16 | 16 | >64 | >64 | blaNDM-12, blaOXA-48, blaCMY-6, blaSHV-106, blaTEM-1 |
CHN24069 | Department of Hematology | Blood | 307 | >64 | 64 | 64 | >64 | >64 | blaNDM-12, blaOXA-48, blaCMY-6, blaCTX-M-15, blaSHV-106, blaTEM-1, blaOXA-1 |
Isolates | MIC (mg/L) 1 | ||||
---|---|---|---|---|---|
ATM | IPM | MEM | ETP | CAZ | |
Conjugation | |||||
EC600 | 0.125 | 0.25 | <0.03 | <0.03 | 0.5 |
E24039J-pOXA-48 | 0.25 | 2 | 1 | 16 | 0.25 |
E24001J-pNDM-1 | >64 | 1 | 0.25 | 2 | >64 |
E24025J-pNDM-1 | >64 | 1 | 0.25 | 4 | >64 |
E24039J-pNDM-1 | >64 | 1 | 0.25 | 8 | >64 |
Gene cloning | |||||
DH5α | <0.03 | 0.25 | <0.03 | <0.03 | 0.125 |
DH5α-pCR2.1K | 0.0625 | 0.25 | <0.03 | <0.03 | 0.25 |
DH5α-pCR2.1K::NDM-1 | 0.0625 | 32 | 64 | 64 | >64 |
DH5α-pCR2.1K::NDM-1(T) 2 | 0.125 | 0.25 | <0.03 | <0.03 | 0.5 |
DH5α-pCR2.1K::CMY-6 | >64 | 1 | 0.25 | 2 | >64 |
DH5α-pCR2.1K::OXA-48 | 0.25 | 2 | 0.5 | 16 | 0.5 |
DH5α-pCR2.1K::NDM-1(T) 2::CMY-6 | >64 | 0.5 | 0.25 | 2 | >64 |
DH5α-pCR2.1K::NDM-1(T) 2::OXA-48 | 0.25 | 2 | 1 | 8 | 0.5 |
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Shi, Q.; Han, X.; Huang, Q.; Meng, Y.; Zhang, P.; Wang, Z.; Hu, H.; Jiang, Y.; Du, X.; Yu, Y. The Genetic Characteristics and Carbapenem Resistance Mechanism of ST307 Klebsiella pneumoniae Coharbouring blaCMY-6, blaOXA-48, and a Truncated blaNDM-1. Antibiotics 2022, 11, 1616. https://doi.org/10.3390/antibiotics11111616
Shi Q, Han X, Huang Q, Meng Y, Zhang P, Wang Z, Hu H, Jiang Y, Du X, Yu Y. The Genetic Characteristics and Carbapenem Resistance Mechanism of ST307 Klebsiella pneumoniae Coharbouring blaCMY-6, blaOXA-48, and a Truncated blaNDM-1. Antibiotics. 2022; 11(11):1616. https://doi.org/10.3390/antibiotics11111616
Chicago/Turabian StyleShi, Qiucheng, Xinhong Han, Qin Huang, Yan Meng, Ping Zhang, Zhengan Wang, Huangdu Hu, Yan Jiang, Xiaoxing Du, and Yunsong Yu. 2022. "The Genetic Characteristics and Carbapenem Resistance Mechanism of ST307 Klebsiella pneumoniae Coharbouring blaCMY-6, blaOXA-48, and a Truncated blaNDM-1" Antibiotics 11, no. 11: 1616. https://doi.org/10.3390/antibiotics11111616